Isolation and characterisation of the potential immunomodulatory principles from Astragalus membranaceus

Masters Thesis


Watson, Robert James. 2006. Isolation and characterisation of the potential immunomodulatory principles from Astragalus membranaceus. Masters Thesis Master of Philosophy. University of Southern Queensland.
Title

Isolation and characterisation of the potential immunomodulatory principles from Astragalus membranaceus

TypeMasters Thesis
Authors
AuthorWatson, Robert James
SupervisorMukkur, T. K. S.
Institution of OriginUniversity of Southern Queensland
Qualification NameMaster of Philosophy
Number of Pages89
Year2006
Abstract

[Abstract]: This thesis describes an in-vivo evaluation of ethanolic extracts of Astragalus membranaceus and fractions derived from this extract administered either orally by gavage or by intraperitoneal injection to Balb/c mice. Total antibody titre was used as an indicator of humoral immune response. Cell mediated immune response was determined using Interferon-γ and Interleukin-12 as indicators. Additionally, mice were vaccinated with a killed Salmonella typhimurium vaccine, previously demonstrated to induce humoral response but not cell mediated immunity, to determine whether the acquired immune response was enhanced or suppressed. Serum was analysed for total antibody titre using an enzyme-linked immunosorbent assay. Serum and splenocyte culture supernatants were analysed for levels of interferon-γ and interleukin-12. No statistically significant differences were observed between groups either orally gavaged or intraperitoneally injected with extracts of Astragalus membranaceus, or orally gavaged with fractions derived from this extract when compared with the control groups.

KeywordsAstragalus membranaceus; immune response
ANZSRC Field of Research 2020320409. Tumour immunology
310905. Animal immunology
Permalink -

https://research.usq.edu.au/item/9yy38/isolation-and-characterisation-of-the-potential-immunomodulatory-principles-from-astragalus-membranaceus

Download files


Published Version
Watson_2006_whole.pdf
File access level: Anyone

  • 2298
    total views
  • 777
    total downloads
  • 2
    views this month
  • 1
    downloads this month

Export as