Molecular marker assisted selection for crown rot resistance in Triticum turgidum ssp. durum

PhD Thesis

Eberhard, Friederike S.. 2011. Molecular marker assisted selection for crown rot resistance in Triticum turgidum ssp. durum. PhD Thesis Doctor of Philosophy. University of Southern Queensland.
Title

Molecular marker assisted selection for crown rot resistance in Triticum turgidum ssp. durum

TypePhD Thesis
Authors
AuthorEberhard, Friederike S.
SupervisorSutherland, Mark
Martin, Anke
Institution of OriginUniversity of Southern Queensland
Qualification NameDoctor of Philosophy
Number of Pages174
Year2011
Abstract

Multicolour fluorescence in situ hybridisation (MCFISH), Simple Sequence Repeat markers (SSR) and Diversity Arrays Technology (DArT) markers were employed in this study to analyse six generations of interspecific wheat crosses between a Crown Rot (CR) resistant hexaploid wheat (Triticum aestivum L. var.2-49) and three tetraploid durum wheat lines (T. turgidum L. spp. durum (Desf.) var. M= 961111 (Bellaroi); N= 950329; O=971179), over the years 2005-2009. In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis carried some D genome material, principally as entire chromosomes, while 40 plants included at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition, two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material.
SSR markers linked to CR seedling resistance in 2-49 in previous studies were useful for the prediction of CR resistance in the crosses. DArT analysis of 191 F7 plants revealed significant QTL for CR on chromosomes 1A and 4B. D chromatin was not responsible for CR resistance, as it was progressively eliminated throughout successive generations.
This study illustrates that the combined application of SSR markers, MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.

Keywordsdurum wheat; crown rot resistance; Triticum turgidum ssp. durum; molecular marker; genome chromosomes; simple sequence repeat markers; SSR; diversity arrays technology markers; DArT; multicolour fluorescence in situ hybridisation; MCFISH
ANZSRC Field of Research 2020300406. Crop and pasture improvement (incl. selection and breeding)
300499. Crop and pasture production not elsewhere classified
Byline AffiliationsCentre for Systems Biology
Permalink -

https://research.usq.edu.au/item/q1z84/molecular-marker-assisted-selection-for-crown-rot-resistance-in-triticum-turgidum-ssp-durum

Log in to edit

Download files

Published Version
Eberhard_2011_whole.pdf
File access level: Anyone

Eberhard_2011_appendix1.pdf
File access level: Anyone

Eberhard_2011_appendix2.pdf
File access level: Anyone

Eberhard_2011_appendix3.pdf
File access level: Anyone

  • 1949
    total views
  • 885
    total downloads
  • 2
    views this month
  • 5
    downloads this month

Export as

Related outputs

Investigation of Gas Release from Recycled Plastic Shopping Bags during Melting at Low Temperatures
Eberhard, Susette, Bowtell, Les, Lenske, Brian and Islam, Mainul. 2023. "Investigation of Gas Release from Recycled Plastic Shopping Bags during Melting at Low Temperatures." Advances in Materials Science and Engineering. 2023, pp. 1-9. https://doi.org/10.1155/2023/2378231
Investigation of ethanol production potential from lignocellulosic material without enzymatic hydrolysis using the ultrasound technique
Kandasamy, Manoj, Hamawand, Ihsan, Bowtell, Leslie, Seneweera, Saman, Chakrabarty, Sayan, Yusaf, Talal, Shakoor, Zaidoon, Algayyim, Sattar and Eberhard, Friederike. 2017. "Investigation of ethanol production potential from lignocellulosic material without enzymatic hydrolysis using the ultrasound technique." Energies. 10 (1), pp. 62-73. https://doi.org/10.3390/en10010062
Influence of yield and other cane characteristics on cane loss and product quality
Khawprateep, S., Jensen, T. A., Schroeder, B. L. and Eberhard, S.. 2016. "Influence of yield and other cane characteristics on cane loss and product quality ." Allsopp, Peter G. (ed.) 29th Congress of the International Society of Sugar Cane Technologists (ISSCT 2016). Chiang Mai, Thailand 05 - 08 Dec 2016
Selective Electrodialysis for Copper Removal from Brackish Water and Coal Seam Gas Water
Eberhard, F. S. and Hamawand, I.. 2017. "Selective Electrodialysis for Copper Removal from Brackish Water and Coal Seam Gas Water." International Journal of Environmental Research. 11 (1), pp. 1-11. https://doi.org/10.1007/s41742-017-0001-y
Towards a custom made water product - potential use of electrodialysis for coal seam gas water treatment using the example of copper ions
Eberhard, Friederike. 2016. "Towards a custom made water product - potential use of electrodialysis for coal seam gas water treatment using the example of copper ions." 2016 International Conference on Biological, Chemical and Environmental Sciences (CES2016) . Tokyo, Japan 14 - 15 Aug 2016 Tokyo, Japan.
Excel table of genotyping data of the markers on the A and B genome chromosomes of eight hexaploid/tetraploid populations
Martin, Anke, Sutherland, Mark W. and Eberhard, Susette. Excel table of genotyping data of the markers on the A and B genome chromosomes of eight hexaploid/tetraploid populations. Toowoomba.
Issues related to waste sewage sludge drying under superheated steam
Hamawand, Ihsan, da Silva, Wilton Pereira, Eberhard, Friederike and Antille, Diogenes L.. 2015. "Issues related to waste sewage sludge drying under superheated steam." Polish Journal of Chemical Technology. 17 (4), pp. 5-14. https://doi.org/10.1515/pjct-2015-0062
A UV absorbance test for measuring humified organic carbon in soil
Eberhard, F. and Pittaway, P.. 2014. "A UV absorbance test for measuring humified organic carbon in soil ." Bruce, R. C. (ed.) 36th Annual Conference of the Australian Society of Sugar Cane Technologists (ASSCT 2014). Gold Coast, Australia 29 Apr - 01 May 2014 Mackay, Australia.
Crown rot of winter cereals: integrating molecular studies and germplasm improvement
Sutherland, Mark W., Bovill, W. D., Eberhard, F. S., Lehmensiek, A., Herde, D. and Simpfendorfer, S.. 2009. "Crown rot of winter cereals: integrating molecular studies and germplasm improvement." Guest, David (ed.) 17th Biennial Australasian Plant Pathology Society Conference (APPS 2009): Plant Health Management: An Integrated Approach. Newcastle, Australia 29 Sep - 01 Oct 2009 Toowoomba, Australia.
Threshold electrolyte concentration relationships for soils from Macarthur River
Bennett, J. McL., Raine, S. R. and Eberhard, F.. 2012. Threshold electrolyte concentration relationships for soils from Macarthur River. Towoomba, Australia. University of Southern Queensland.
Threshold electrolyte concentration relationships for soils from Roma fields Roleen property
Bennett, J. McL., Raine, S. R. and Eberhard, F.. 2012. Threshold electrolyte concentration relationships for soils from Roma fields Roleen property. Towoomba, Australia. University of Southern Queensland.
Retention of D genome chromosomes in pentaploid wheat crosses
Martin, A., Simpfendorfer, S., Hare, R. A., Eberhard, F. S. and Sutherland, M. W.. 2011. "Retention of D genome chromosomes in pentaploid wheat crosses." Heredity. 107 (4), pp. 315-319. https://doi.org/10.1038/hdy.2011.17
Threshold electrolyte concentration relationships for soils from property A and B
Bennett, J., Raine, S. and Eberhard, F.. 2011. Threshold electrolyte concentration relationships for soils from property A and B. Toowoomba, Australia. University of Southern Queensland.
Threshold electrolyte concentration relationships for soils from Roleen, Tantatton, Pine Ridge, Ben Bow and Oakleigh
Bennett, J., Raine, S. and Eberhard, F.. 2011. Threshold electrolyte concentration relationships for soils from Roleen, Tantatton, Pine Ridge, Ben Bow and Oakleigh. Toowoomba, Australia. University of Southern Queensland.
Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH
Eberhard, Friederike S., Zhang, Peng, Lehmensiek, Anke, Hare, Ray A., Simpfendorfer, Steven and Sutherland, Mark W.. 2010. "Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH." Crop and Pasture Science. 61 (8), pp. 619-624. https://doi.org/10.1071/CP10131
Chromosome composition in an F2 hexaploid x durum cross analysed by DArT markers and MCFISH
Eberhard, F. S., Zhang, P., Lehmensiek, A., Sutherland, M. W., Hare, R. and Simpfendorfer, S.. 2008. "Chromosome composition in an F2 hexaploid x durum cross analysed by DArT markers and MCFISH." Appels, Rudi, Eastwood, Russell, Lagudah, Evans, Mackay, Michael, McIntyre, Lynne, Sharp, Peter and Langridge, Peter (ed.) 11th International Wheat Genetics Sympoium (IWGS 2008). Brisbane, Australia 24 - 29 Aug 2008 Sydney, Australia.
The application of molecular markers for partial resistance against fusarium crown rot in hexaploid and tetraploid wheats
Sutherland, Mark, Bovill, W. D., Horne, M., Lehmensiek, A., Eberhard, F., Percy, C., Wildermuth, G. B., Simpfendorfer, S. and Hare, R.. 2008. "The application of molecular markers for partial resistance against fusarium crown rot in hexaploid and tetraploid wheats." 10th International Fusarium and Fusarium Genomics Workshop 2008. Sardinia, Italy 30 Aug - 02 Sep 2008 Sardinia,Italy.