Efficacy of a competitive enzyme-linked immunosorbent assay (cELISA) for estimating prevalence of immunity to rabbit haemorrhagic disease virus (RHDV) in populations of Australian wild rabbits (Oryctolagus cuniculus)

Article


McPhee, S. R., Berman, D., Gonzales, A., Butler, K. L., Humphrey, J., Muller, J., Waddington, J. N., Koch, S., Daniels, P. and Marks, C. A.. 2002. "Efficacy of a competitive enzyme-linked immunosorbent assay (cELISA) for estimating prevalence of immunity to rabbit haemorrhagic disease virus (RHDV) in populations of Australian wild rabbits (Oryctolagus cuniculus)." Wildlife Research. 29 (6), pp. 635-647. https://doi.org/10.1071/WR00114
Article Title

Efficacy of a competitive enzyme-linked immunosorbent assay (cELISA) for estimating prevalence of immunity to rabbit haemorrhagic disease virus (RHDV) in populations of Australian wild rabbits (Oryctolagus cuniculus)

ERA Journal ID3020
Article CategoryArticle
AuthorsMcPhee, S. R. (Author), Berman, D. (Author), Gonzales, A. (Author), Butler, K. L. (Author), Humphrey, J. (Author), Muller, J. (Author), Waddington, J. N. (Author), Koch, S. (Author), Daniels, P. (Author) and Marks, C. A. (Author)
Journal TitleWildlife Research
Journal Citation29 (6), pp. 635-647
Number of Pages13
Year2002
PublisherCSIRO Publishing
Place of PublicationAustralia
ISSN1035-3712
1448-5494
Digital Object Identifier (DOI)https://doi.org/10.1071/WR00114
Web Address (URL)http://www.publish.csiro.au/wr/WR00114
Abstract

This study examines the efficacy of a cELISA in estimating the prevalence of immunity to rabbit haemorrhagic disease virus (RHDV) in wild rabbits in Australia. Rabbits (n = 343) captured from six locations in Victoria and Queensland were experimentally challenged with a lethal oral dose (1500 50%-lethal doses, LD50) of RHDV. Death or survival to challenge was used to determine the performance characteristics of the test. The diagnostic specificity, sensitivity and accuracy were highly variable between sites, making it difficult to select a representative cut-off value for all sites that achieved a reasonable level of accuracy for the prediction of surviving and non-surviving rabbits. Estimates of prevalence of immunity were biased owing to effects of site of capture (time of capture) and age structure of the population. Using predictive equations, the best estimates of survival were ±10% but these results came from a limited range of sites, all of which had survival in the range 49–70%. The cELISA will determine whether the RHDV is present in rabbit populations but it should be used with caution when estimating the prevalence of immunity to RHDV. The cELISA may thus be limited in its application for examining the epidemiology of RHDV in Australian rabbit populations.

Keywordswild rabbit, rabbit hemorrhagic disease, immunity
ANZSRC Field of Research 2020410499. Environmental management not elsewhere classified
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Byline AffiliationsAustralian Centre for International Agricultural Research
Department of Natural Resources and Mines, Queensland
Department of Natural Resources, Queensland
Agriculture Victoria
Department of Primary Industries and Fisheries, Northern Territory
Department of Land Environment Water and Planning, Victoria
Institution of OriginUniversity of Southern Queensland
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