Determining the genetic characteristics of Didymella arachidicola and its peanut host

PhD by Publication


Wood, Shona Elizabeth. 2023. Determining the genetic characteristics of Didymella arachidicola and its peanut host. PhD by Publication Doctor of Philosophy. University of Southern Queensland. https://doi.org/10.26192/z1x2w
Title

Determining the genetic characteristics of Didymella arachidicola and its peanut host

TypePhD by Publication
AuthorsWood, Shona Elizabeth
Supervisor
1. FirstProf Gavin Ash
2. SecondDr Barsha Poudel
3. ThirdMark Dieters
Graeme Wright
Manish K. Pandey
Institution of OriginUniversity of Southern Queensland
Qualification NameDoctor of Philosophy
Number of Pages157
Year2023
PublisherUniversity of Southern Queensland
Place of PublicationAustralia
Digital Object Identifier (DOI)https://doi.org/10.26192/z1x2w
Abstract

Didymella arachidicola is the necrotrophic causative agent of net blotch in peanuts (Arachis hypogaea L.). It reduces yields and quality in Australia and many cooler production regions globally. Host resistance is generally thought of as the most effective form of control, however little has been studied of the pathogen itself and the genetic sources of host resistance. To effectively breed for durable resistance in a commercially viable peanut cultivar, it is necessary to understand the genetic diversity of D. arachidicola. This was achieved through hierarchically sampling a single peanut field in Queensland, Australia and investigating the genetic diversity through Diversity Array Technology, and the population structure of 84 isolates. The population structure of D. arachidicola indicated that the population consisted of one group with a high level of genetic diversity, containing 57 multi locus genotypes. This indicated that a single isolate of D. arachidicola was suitable to represent the diversity contained within a field. In addition, this study utilised whole genome sequences of two D. arachidicola strains to confirm homothallism. Nineteen peanut lines were evaluated under field and glasshouse conditions (winter and summer) to determine if phenotyping in the glasshouse related to the field disease response. A high level of correlation was demonstrated between nine-week-old plants in both glasshouse experiments and phenotyping in the field. To further understand host resistance, a total of 406 recombinant inbred lines segregating for net blotch resistance were phenotyped in the field and glasshouse and genotyped on a high-density SNP chip. Combining the phenotypic and genotypic data identified a total of 55 correlated quantitative trait loci (QTL). One QTL, qtlNB02A.3, explained 17.03% of the phenotypic variation. This will benefit peanut breeding programs enabling them to select for net blotch resistance using molecular tools. Once a net blotch resistant peanut genotype is developed there is a high chance that the resistance will be durable, as within the host there are several minor effect genomic regions involved in minimising the disease and there are minimal genetic populations within the pathogen.

Keywordsgenetic diversity; Peanut; pathology; Didymella arachidicola
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020300210. Sustainable agricultural development
300406. Crop and pasture improvement (incl. selection and breeding)
Public Notes

File reproduced in accordance with the copyright policy of the publisher/author.

Byline AffiliationsInstitute for Life Sciences and the Environment
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