Identification, validation, and pyramiding of quantitative trait loci for resistance to crown rot in wheat

PhD Thesis


Bovill, William D.. 2007. Identification, validation, and pyramiding of quantitative trait loci for resistance to crown rot in wheat. PhD Thesis Doctor of Philosophy. University of Southern Queensland.
Title

Identification, validation, and pyramiding of quantitative trait loci for resistance to crown rot in wheat

TypePhD Thesis
Authors
AuthorBovill, William D.
SupervisorSutherland, Mark
Institution of OriginUniversity of Southern Queensland
Qualification NameDoctor of Philosophy
Number of Pages202
Year2007
Abstract

[Abstract]: Crown rot (causal organism: Fusarium pseudograminearum) is a significant
disease affecting wheat in Australia. Although first reported over 60 years ago, the disease has become more prevalent in recent years due to the adoption of
minimum tillage and stubble retention practices. Breeding for resistance to crown rot is difficult - phenotypic selection, which is usually done at harvest, is
time-consuming, expensive, and subject to between year variability due to sensitivity to environmental conditions. For these reasons, the coupling of
molecular techniques with conventional plant breeding (marker-assisted selection) has the potential to more rapidly and reliably identify genomic regions
that contribute to resistance. The objective of this study was to identify, validate,
and pyramid quantitative trait loci (QTL) for resistance to crown rot present in a
W21MMT70 x Mendos doubled haploid wheat population.

Replicated seedling trials were conducted in 2001, 2003, and 2005. In each
seedling trial, W21MMT70 displayed partial resistance to crown rot whereas
Mendos seedlings were susceptible. A bulked segregant analysis (BSA), using
390 simple sequence repeat (SSR) markers chosen for their coverage of the
wheat genome, was initially conducted based upon the 2001 seedling trial data in
an attempt to rapidly identify genomic regions associated to resistance. The BSA
did not reveal any markers associated with resistance to crown rot. As a result, a
full mapping study was conducted. One hundred and twenty eight (128) SSR
markers were mapped across the population to produce a framework map.
Previously screened AFLP markers were added to the map. Composite interval
mapping revealed eight QTL associated with resistance. Of these, three (located
on chromosomes 2B, 2D, and 5D) were consistently detected in each of the three
seedling trials. Two QTL (on chromosomes 1A and 3B) were detected in two of
the three trials. The 2D, 3B, and 5D QTL were inherited from W21MMT70,
whereas the 1A and 2B QTL were inherited from Mendos.

Two software programs were used to identify epistatic interactions between
QTL. While the results of the two programs differed markedly, both programs
detected a highly significant interaction between the W21MMT70 inherited 5D
QTL and a locus on chromosome 2D inherited from Mendos. The overall effect
of the epistatic interactions was not as great as the additive effects of nonepistatic
QTL. Nonetheless, the presence of epistasis may indicate that,
particularly in the case of 5D, the effect of this QTL may be dependent on the
background into which it is introgressed.

Validation of three W21MMT70-inherited QTL (on chromosomes 2D, 3B, and
5D) was conducted on three F2 populations with W21MMT70 as one of the
parents. While the 5D QTL was validated in two of the three crosses, neither the
2D nor the 3B QTL were detected in any of the F2 validation populations. It is
likely that the size of the F2 populations (the largest composed of 94 individuals),
in conjunction with the variability that is inherent when screening for resistance
to crown rot, precluded validation of these regions. Validation of the 2B
Mendos-inherited QTL was conducted on a Sunco x Batavia doubled haploid
population because Sunco possesses the same Triticum timopheevi 2B
introgression that is present in Mendos. This validated QTL (designated
Q.CR..usq-2B2) explained 11 % of the phenotypic variance in the Sunco x
Batavia population.

To assess the effectiveness of pyramiding QTL for resistance to crown rot, a 2-49
x W21MMT70 population was examined. A number of lines of this population
performed significantly better than each of the parents in the replicated seedling
trial that was conducted. Four QTL, located on chromosomes 1A, 1D, 2D, and
3B, were detected. The 1A and 1D QTL were inherited from 2-49 whereas the
2D and 3B QTL were inherited from W21MMT70. The 1A QTL from 2-49 has
not been previously validated, and this QTL has been designated QCr.usq-1A1.
The 3B QTL (designated QCr.usq-3B1) had the highest effect (LRS 42.1;
explaining 21.0 % of the phenotypic variance) in the 2-49 x W21MMT70
population. The 2D QTL (QCr.usq-2D1) was shown to have a minor effect. The
5D QTL that was inherited from W21MMT70 in the W21MMT70 x Mendos
population was not detected in the 2-49 x W21MMT70 population. A number of
possible explanations for the inability to detect this QTL in the 2-49 x
W21MMT70 population are discussed.

Keywordscrown rot; wheat; Australia; quantitative trait loci
ANZSRC Field of Research 2020300409. Crop and pasture protection (incl. pests, diseases and weeds)
300499. Crop and pasture production not elsewhere classified
Byline AffiliationsCentre for Systems Biology
Permalink -

https://research.usq.edu.au/item/9y7yx/identification-validation-and-pyramiding-of-quantitative-trait-loci-for-resistance-to-crown-rot-in-wheat

Download files


Published Version
Bovill_2007_whole.pdf
File access level: Anyone

  • 2350
    total views
  • 1141
    total downloads
  • 1
    views this month
  • 2
    downloads this month

Export as

Related outputs

Markers for seedling and adult plant crown rot resistance in four partially resistant bread wheat sources
Martin, A., Bovill, W. D., Percy, C. D., Herde, D., Fletcher, S., Kelly, A., Neate, S. M. and Sutherland, M. W.. 2015. "Markers for seedling and adult plant crown rot resistance in four partially resistant bread wheat sources." Theoretical and Applied Genetics: international journal of plant breeding research. 128 (3), pp. 377-385. https://doi.org/10.1007/s00122-014-2437-1
Crown rot of winter cereals: integrating molecular studies and germplasm improvement
Sutherland, Mark W., Bovill, W. D., Eberhard, F. S., Lehmensiek, A., Herde, D. and Simpfendorfer, S.. 2009. "Crown rot of winter cereals: integrating molecular studies and germplasm improvement." Guest, David (ed.) 17th Biennial Australasian Plant Pathology Society Conference (APPS 2009): Plant Health Management: An Integrated Approach. Newcastle, Australia 29 Sep - 01 Oct 2009 Toowoomba, Australia.
Wheat flour protein content and water absorption analysis in a doubled haploid population
Ma, Wujun, Sutherland, Mark W., Kammholz, Stephen, Banks, Phillip, Brennan, Paul, Bovill, William and Daggard, Grant. 2007. "Wheat flour protein content and water absorption analysis in a doubled haploid population." Journal of Cereal Science. 45 (3), pp. 302-308. https://doi.org/10.1016/j.jcs.2006.10.005
Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49'
Collard, B. C. Y., Jolley, R., Bovill, W. D., Grams, R. A., Wildermuth, G. B. and Sutherland, M. W.. 2006. "Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49'." Australian Journal of Agricultural Research. 57 (9), pp. 967-973. https://doi.org/10.1071/AR05419
Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a '2-49' x 'Janz' population
Collard, B. C. Y., Grams, R. A., Bovill, W. D., Percy, C. D., Jolley, R., Lehmensiek, A., Wildermuth, G. B. and Sutherland, M. W.. 2005. "Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a '2-49' x 'Janz' population." Plant Breeding. 124 (6), pp. 532-537. https://doi.org/10.1111/j.1439-0523.2005.01163.x
Molecular characterization of a Triticum timopheevii introgression in a Wentworth/Lang population
Lehmensiek, A., Bovill, W. D., Banks, P. M. and Sutherland, M. W.. 2008. "Molecular characterization of a Triticum timopheevii introgression in a Wentworth/Lang population." Appels, Rudi (ed.) 11th International Wheat Genetics Symposium 2008 (IWGS 2008). Brisbane, Australia 24 - 29 Aug 2008 Sydney, Australia.
Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)
Bovill, W. D., Horne, M., Herde, D., Davis, M., Wildermuth, G. B. and Sutherland, M. W.. 2010. "Pyramiding QTL increases seedling resistance to crown rot (Fusarium pseudograminearum) of wheat (Triticum aestivum)." Theoretical and Applied Genetics: international journal of plant breeding research. 121 (1), pp. 127-136. https://doi.org/10.1007/s00122-010-1296-7
Genetic mapping in the Triticeae
Lehmensiek, Anke, Bovill, William, Wenzl, Peter, Langridge, Peter and Appels, Rudi. 2009. "Genetic mapping in the Triticeae." Feuillet, Catherine and Muehlbauer, Gary (ed.) Genetics and genomics of the Triticeae. New York, NY. United States. Springer. pp. 201-235
The application of molecular markers for partial resistance against fusarium crown rot in hexaploid and tetraploid wheats
Sutherland, Mark, Bovill, W. D., Horne, M., Lehmensiek, A., Eberhard, F., Percy, C., Wildermuth, G. B., Simpfendorfer, S. and Hare, R.. 2008. "The application of molecular markers for partial resistance against fusarium crown rot in hexaploid and tetraploid wheats." 10th International Fusarium and Fusarium Genomics Workshop 2008. Sardinia, Italy 30 Aug - 02 Sep 2008 Sardinia,Italy.
Mapping markers for priority traits in the northern grains region of Australia
Mace, E., Sutherland, Mark, Lehmensiek, Anke, Bovill, Bill, Bovill, Jessica, Collard, Bert, Daggard, Grant and Christopher, M. J.. 2006. "Mapping markers for priority traits in the northern grains region of Australia." ITMI Workshop and ACPFG Genomics Symposium (2006). Victor Harbour, Australia 27 - 31 Aug 2006
Identification of novel QTL for resistance to crown rot in the doubled haploid wheat population 'W21MMT70' x 'Mendos'
Bovill, W. D., Ma, W., Ritter, K., Collard, B. C. Y., Davis, M., Wildermuth, G. B. and Sutherland, M. W.. 2006. "Identification of novel QTL for resistance to crown rot in the doubled haploid wheat population 'W21MMT70' x 'Mendos' ." Plant Breeding. 125 (6), pp. 538-543. https://doi.org/10.1111/j.1439-0523.2006.01251.x
Strategies for fine mapping QTLs for crown rot resistance in wheats
Bovill, W. D., Collard, B. C. Y., Lehmensiek, Anke, Wildermuth, G. B. and Sutherland, Mark W.. 2005. "Strategies for fine mapping QTLs for crown rot resistance in wheats." Cahill, David (ed.) 15th Biennial Australasian Plant Pathology Society Conference. Geelong, Australia 26 - 29 Sep 2005 Geelong.