Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49'
Article
Article Title | Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line '2-49' |
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ERA Journal ID | 5177 |
Article Category | Article |
Authors | Collard, B. C. Y. (Author), Jolley, R. (Author), Bovill, W. D. (Author), Grams, R. A. (Author), Wildermuth, G. B. (Author) and Sutherland, M. W. (Author) |
Journal Title | Australian Journal of Agricultural Research |
Journal Citation | 57 (9), pp. 967-973 |
Number of Pages | 7 |
Year | 2006 |
Publisher | CSIRO Publishing |
Place of Publication | Australia |
ISSN | 0004-9409 |
1444-9838 | |
Digital Object Identifier (DOI) | https://doi.org/10.1071/AR05419 |
Web Address (URL) | https://www.publish.csiro.au/cp/AR05419 |
Abstract | We have tested the efficacy of putative microsatellite single sequence repeat (SSR) markers, previously identified in a 2-49 (Gluyas Early/Gala) x Janz doubled haploid wheat (Triticum aestivum) population, as being linked to partial seedling resistance to crown rot disease caused by Fusarium pseudograminearum. The quantitative trait loci (QTLs) delineated by these markers have been tested for linkage to resistance in an independent Gluyas Early x Janz doubled haploid population. The presence of a major QTL on chromosome 1DL (QCr.usq-1D1) and a minor QTL on chromosome 2BS (QCr.usq-2B1) was confirmed. However, a putative minor QTL on chromosome 2A was not confirmed. The QTL on 1D was inherited from Gluyas Early, a direct parent of 2-49, whereas the 2B QTL was inherited from Janz. Three other putative QTLs identified in 2-49 x Janz (on 1AL, 4BL, and 7BS) were inherited by 2-49 from Gala and were not able to be confirmed in this study. The screening of SSR markers on a small sample of elite wheat genotypes indicated that not all of the most tightly linked SSR markers flanking the major QTLs on 1D and 1A were polymorphic in all backgrounds, indicating the need for additional flanking markers when backcrossing into some elite pedigrees. Comparison of SSR haplotypes with those of other genotypes exhibiting partial crown rot resistance suggests that additional, novel sources of crown rot resistance are available. |
Keywords | targetted mapping; QTL resolution; 1-LOD support interval; allelic homoplasy |
ANZSRC Field of Research 2020 | 310506. Gene mapping |
300406. Crop and pasture improvement (incl. selection and breeding) | |
300409. Crop and pasture protection (incl. pests, diseases and weeds) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Centre for Systems Biology |
Department of Primary Industries, Queensland |
https://research.usq.edu.au/item/9xvx1/confirmation-of-qtl-mapping-and-marker-validation-for-partial-seedling-resistance-to-crown-rot-in-wheat-line-2-49
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