Relationship between transembrane ion movements, production of reactive oxygen species and the hypersensitive response during the challenge of tobacco suspension cells by zoospores of Phytophthora nicotianae
Article
Article Title | Relationship between transembrane ion movements, production of reactive oxygen species and the hypersensitive response during the challenge of tobacco suspension cells by zoospores of Phytophthora nicotianae |
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ERA Journal ID | 2635 |
Article Category | Article |
Authors | Able, Amanda J. (Author), Guest, David I. (Author) and Sutherland, Mark W. (Author) |
Journal Title | Physiological and Molecular Plant Pathology |
Journal Citation | 58 (5), pp. 189-198 |
Number of Pages | 10 |
Year | 2001 |
Place of Publication | United Kingdom |
ISSN | 0885-5765 |
1096-1178 | |
Digital Object Identifier (DOI) | https://doi.org/10.1006/pmpp.2001.0328 |
Web Address (URL) | https://www.sciencedirect.com/science/article/abs/pii/S0885576501903285 |
Abstract | We have examined the responses of suspension cultured tobacco cells after inoculation with zoospores from compatible or incompatible races of the Oomycete pathogen Phytophthora nicotianae. The incompatible interaction, characterized by the production of reactive oxygen species (ROS) (specifically superoxide (<$>{\rm HO^{\,\cdot}_{2}/O_{2}^{\minus}}<$>) and hydrogen peroxide (H2O2)), and by a hypersensitive response (HR), was not dependent upon the presence of exogenous calcium. However, perturbation of calcium ion movements by EGTA or LaCl3suppressed both ROS production and the extent of cell death. The Ca2+-specific ionophore, A23187, slightly enhanced ROS production during the incompatible interaction but had no effect on the responses of susceptible or unchallenged cells. These results confirm that both ROS production and the HR are potentiated by movement of endogenous calcium across the plasmalemma. While there was no evidence of a transmembrane K+/H+exchange immediately following incompatible zoospore challenge, a later potassium efflux from cells coincided with the onset of the HR. Unexpectedly, medium pH drifted downwards during all host cell responses suggesting that alkaline peroxidases are not the major source of ROS generation during an incompatible tobacco cell/zoospore interaction. |
Keywords | calcium, hydrogen peroxide, HR, Nicotiana tabacum, Phytophthora nicotianae, plant-, pathogen interactions, ROS, superoxide, XTT, potassium, pH |
ANZSRC Field of Research 2020 | 310805. Plant pathology |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Faculty of Sciences |
University of Sydney | |
Department of Biological and Physical Sciences |
https://research.usq.edu.au/item/9y5yq/relationship-between-transembrane-ion-movements-production-of-reactive-oxygen-species-and-the-hypersensitive-response-during-the-challenge-of-tobacco-suspension-cells-by-zoospores-of-phytophthora
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