Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro

Article


Pereira, Brooke A., Lister, Natalie L., Hashimoto, Kohei, Teng, Linda, Flandes-Iparraguirre, Maria, Eder, Angelina, Sanchez-Herrero, Alvaro, Niranjan, Birunthi, Frydenberg, Mark, Papargiris, Melissa M., Lawrence, Mitchell G., Taylor, Renea A., Hutmacher, Dietmar W., Ellem, Stuart J., Risbridger, Gail P. and De-Juan-Pardo, Elena M.. 2019. "Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro." Biomaterials. 197, pp. 72-85. https://doi.org/10.1016/j.biomaterials.2018.12.030
Article Title

Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro

ERA Journal ID5035
Article CategoryArticle
AuthorsPereira, Brooke A. (Author), Lister, Natalie L. (Author), Hashimoto, Kohei (Author), Teng, Linda (Author), Flandes-Iparraguirre, Maria (Author), Eder, Angelina (Author), Sanchez-Herrero, Alvaro (Author), Niranjan, Birunthi (Author), Frydenberg, Mark (Author), Papargiris, Melissa M. (Author), Lawrence, Mitchell G. (Author), Taylor, Renea A. (Author), Hutmacher, Dietmar W. (Author), Ellem, Stuart J. (Author), Risbridger, Gail P. (Author) and De-Juan-Pardo, Elena M. (Author)
Journal TitleBiomaterials
Journal Citation197, pp. 72-85
Number of Pages14
Year2019
PublisherElsevier
Place of PublicationNetherlands
ISSN0142-9612
1878-5905
Digital Object Identifier (DOI)https://doi.org/10.1016/j.biomaterials.2018.12.030
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0142961218308652
Abstract

The tumour microenvironment plays a vital role in the development of solid malignancies. Here we describe an in vitro human prostate cancer microtissue model that facilitates the incorporation and interrogation of key elements of the local prostatic tumour microenvironment. Primary patient-derived cancer-associated fibroblasts (CAFs) were cultured in three-dimensional (3D) melt electrowritten scaffolds where they deposited extensive extracellular matrix (ECM) and promoted significant changes in prostate epithelial morphology, when compared to matched non-malignant prostatic fibroblasts (NPFs). The addition of mast cells, a resident prostatic immune population that is expanded during early malignancy, enhanced the morphometric transition of benign epithelia via a tryptase-mediated mechanism. Our patient-specific 3D microtissues reveal a cascade of interactions between prostatic CAFs, their native ECM and mast cell-derived tryptase, rendering them important microenvironmental drivers of prostate cancer progression.

Keywordsprostate cancer; cancer-associated fibroblasts; melt electrowritten scaffolds; tumour microenvironment; mast cells 3D model
ANZSRC Field of Research 2020321101. Cancer cell biology
310105. Cellular interactions (incl. adhesion, matrix, cell wall)
400302. Biomaterials
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Byline AffiliationsMonash University
Sapporo Medical University, Japan
Queensland University of Technology
Victorian Comprehensive Cancer Centre, Australia
School of Health and Wellbeing
University of Melbourne
Institution of OriginUniversity of Southern Queensland
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