Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro
Article
Article Title | Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro |
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ERA Journal ID | 5035 |
Article Category | Article |
Authors | Pereira, Brooke A. (Author), Lister, Natalie L. (Author), Hashimoto, Kohei (Author), Teng, Linda (Author), Flandes-Iparraguirre, Maria (Author), Eder, Angelina (Author), Sanchez-Herrero, Alvaro (Author), Niranjan, Birunthi (Author), Frydenberg, Mark (Author), Papargiris, Melissa M. (Author), Lawrence, Mitchell G. (Author), Taylor, Renea A. (Author), Hutmacher, Dietmar W. (Author), Ellem, Stuart J. (Author), Risbridger, Gail P. (Author) and De-Juan-Pardo, Elena M. (Author) |
Journal Title | Biomaterials |
Journal Citation | 197, pp. 72-85 |
Number of Pages | 14 |
Year | 2019 |
Publisher | Elsevier |
Place of Publication | Netherlands |
ISSN | 0142-9612 |
1878-5905 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.biomaterials.2018.12.030 |
Web Address (URL) | https://www.sciencedirect.com/science/article/pii/S0142961218308652 |
Abstract | The tumour microenvironment plays a vital role in the development of solid malignancies. Here we describe an in vitro human prostate cancer microtissue model that facilitates the incorporation and interrogation of key elements of the local prostatic tumour microenvironment. Primary patient-derived cancer-associated fibroblasts (CAFs) were cultured in three-dimensional (3D) melt electrowritten scaffolds where they deposited extensive extracellular matrix (ECM) and promoted significant changes in prostate epithelial morphology, when compared to matched non-malignant prostatic fibroblasts (NPFs). The addition of mast cells, a resident prostatic immune population that is expanded during early malignancy, enhanced the morphometric transition of benign epithelia via a tryptase-mediated mechanism. Our patient-specific 3D microtissues reveal a cascade of interactions between prostatic CAFs, their native ECM and mast cell-derived tryptase, rendering them important microenvironmental drivers of prostate cancer progression. |
Keywords | prostate cancer; cancer-associated fibroblasts; melt electrowritten scaffolds; tumour microenvironment; mast cells 3D model |
ANZSRC Field of Research 2020 | 321101. Cancer cell biology |
310105. Cellular interactions (incl. adhesion, matrix, cell wall) | |
400302. Biomaterials | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Monash University |
Sapporo Medical University, Japan | |
Queensland University of Technology | |
Victorian Comprehensive Cancer Centre, Australia | |
School of Health and Wellbeing | |
University of Melbourne | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q5032/tissue-engineered-human-prostate-microtissues-reveal-key-role-of-mast-cellderived-tryptase-in-potentiating-cancer-associated-fibroblast-caf-induced-morphometric-transition-in-vitro
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