Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro

Article


Pereira, Brooke A., Lister, Natalie L., Hashimoto, Kohei, Teng, Linda, Flandes-Iparraguirre, Maria, Eder, Angelina, Sanchez-Herrero, Alvaro, Niranjan, Birunthi, Frydenberg, Mark, Papargiris, Melissa M., Lawrence, Mitchell G., Taylor, Renea A., Hutmacher, Dietmar W., Ellem, Stuart J., Risbridger, Gail P. and De-Juan-Pardo, Elena M.. 2019. "Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro." Biomaterials. 197, pp. 72-85. https://doi.org/10.1016/j.biomaterials.2018.12.030
Article Title

Tissue engineered human prostate microtissues reveal key role of mast cellderived tryptase in potentiating cancer-associated fibroblast (CAF)-induced morphometric transition in vitro

ERA Journal ID5035
Article CategoryArticle
AuthorsPereira, Brooke A. (Author), Lister, Natalie L. (Author), Hashimoto, Kohei (Author), Teng, Linda (Author), Flandes-Iparraguirre, Maria (Author), Eder, Angelina (Author), Sanchez-Herrero, Alvaro (Author), Niranjan, Birunthi (Author), Frydenberg, Mark (Author), Papargiris, Melissa M. (Author), Lawrence, Mitchell G. (Author), Taylor, Renea A. (Author), Hutmacher, Dietmar W. (Author), Ellem, Stuart J. (Author), Risbridger, Gail P. (Author) and De-Juan-Pardo, Elena M. (Author)
Journal TitleBiomaterials
Journal Citation197, pp. 72-85
Number of Pages14
Year2019
PublisherElsevier
Place of PublicationNetherlands
ISSN0142-9612
1878-5905
Digital Object Identifier (DOI)https://doi.org/10.1016/j.biomaterials.2018.12.030
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S0142961218308652
Abstract

The tumour microenvironment plays a vital role in the development of solid malignancies. Here we describe an in vitro human prostate cancer microtissue model that facilitates the incorporation and interrogation of key elements of the local prostatic tumour microenvironment. Primary patient-derived cancer-associated fibroblasts (CAFs) were cultured in three-dimensional (3D) melt electrowritten scaffolds where they deposited extensive extracellular matrix (ECM) and promoted significant changes in prostate epithelial morphology, when compared to matched non-malignant prostatic fibroblasts (NPFs). The addition of mast cells, a resident prostatic immune population that is expanded during early malignancy, enhanced the morphometric transition of benign epithelia via a tryptase-mediated mechanism. Our patient-specific 3D microtissues reveal a cascade of interactions between prostatic CAFs, their native ECM and mast cell-derived tryptase, rendering them important microenvironmental drivers of prostate cancer progression.

Keywordsprostate cancer; cancer-associated fibroblasts; melt electrowritten scaffolds; tumour microenvironment; mast cells 3D model
ANZSRC Field of Research 2020321101. Cancer cell biology
310105. Cellular interactions (incl. adhesion, matrix, cell wall)
400302. Biomaterials
Public Notes

Files associated with this item cannot be displayed due to copyright restrictions.

Byline AffiliationsMonash University
Sapporo Medical University, Japan
Queensland University of Technology
Victorian Comprehensive Cancer Centre, Australia
School of Health and Wellbeing
University of Melbourne
Institution of OriginUniversity of Southern Queensland
Permalink -

https://research.usq.edu.au/item/q5032/tissue-engineered-human-prostate-microtissues-reveal-key-role-of-mast-cellderived-tryptase-in-potentiating-cancer-associated-fibroblast-caf-induced-morphometric-transition-in-vitro

  • 170
    total views
  • 12
    total downloads
  • 2
    views this month
  • 0
    downloads this month

Export as

Related outputs

The development of a modular design workflow for 3D printable bioresorbable patient-specific bone scaffolds to facilitate clinical translation
Herath, Buddhi, Laubach, Markus, Suresh, Sinduja, Schmutz, Beat, Little, J. Paige, Yarlagadda, Prasad K. D. V., Hutmacher, Dietmar W. and Wille, Marie-Luis. 2023. "The development of a modular design workflow for 3D printable bioresorbable patient-specific bone scaffolds to facilitate clinical translation." Virtual and Physical Prototyping. 18 (1). https://doi.org/10.1080/17452759.2023.2246434
Prostate cancer survivorship essentials framework: guidelines for practitioners
Dunn, Jeff, Green, Anna, Ralph, Nicholas, Newton, Robert U., Kneebone, Andrew, Frydenberg, Mark and Chambers, Suzanne K.. 2021. "Prostate cancer survivorship essentials framework: guidelines for practitioners." BJU International. 128 (S3), pp. 18-29. https://doi.org/10.1111/bju.15159
Correction to: Navigate: a study protocol for a randomised controlled trial of an online treatment decision aid for men with low-risk prostate cancer and their partners
Schofield, Penelope, Gough, Karla, Hyatt, Amelia, White, Alan, Frydenberg, Mark, Chambers, Suzanne, Gordon, Louisa G., Gardiner, Robert, Murphy, Declan G., Cavedon, Lawrence, Richards, Natalie, Murphy, Barbara, Quinn, Stephen and Juraskova, Ilona. 2021. "Correction to: Navigate: a study protocol for a randomised controlled trial of an online treatment decision aid for men with low-risk prostate cancer and their partners." Trials. 22. https://doi.org/10.1186/s13063-021-05070-6
Navigate: a study protocol for a randomised controlled trial of an online treatment decision aid for men with low-risk prostate cancer and their partners
Schofield, Penelope, Gough, Karla, Hyatt, Amelia, White, Alan, Frydenberg, Mark, Chambers, Suzanne, Gordon, Louisa G., Gardiner, Robert, Murphy, Declan G., Cavedon, Lawrence, Richards, Natalie, Murphy, Barbara, Quinn, Stephen and Juraskova, Ilona. 2021. "Navigate: a study protocol for a randomised controlled trial of an online treatment decision aid for men with low-risk prostate cancer and their partners." Trials. 22, pp. 1-12. https://doi.org/10.1186/s13063-020-04986-9
Mindfulness-Based Cognitive Therapy in Advanced Prostate Cancer: A Randomized Controlled Trial
Chambers, Suzanne K., Occhipinti, Stefano, Foley, Elizabeth, Clutton, Samantha, Legg, Melissa, Berry, Martin, Stockler, Martin R., Frydenberg, Mark, Gardiner, Robert A., Lepore, Stephen J., Davis, Ian D. and Smith, David P.. 2017. "Mindfulness-Based Cognitive Therapy in Advanced Prostate Cancer: A Randomized Controlled Trial." Journal of Clinical Oncology. 35 (3), pp. 291-297. https://doi.org/10.1200/JCO.2016.68.8788
Work after prostate cancer: a systematic review
McLennan, Vanette, Ludvik, Dominika, Chambers, Suzanne and Frydenberg, Mark. 2019. "Work after prostate cancer: a systematic review." Journal of Cancer Survivorship. 13, pp. 282-291. https://doi.org/10.1007/s11764-019-00750-4
Oxytocin receptor antagonists as a novel pharmacological agent for reducing smooth muscle tone in the human prostate
Lee, Sophie N., Kraska, Jenna, Papargiris, Melissa, Teng, Lind, Niranjan, Birunthi, Hammar, Johanna, Ryan, Andrew, Frydenberg, Mark, Lawrentschuk, Nathan, Middendorff, Ralf, Ellem, Stuart J., Whittaker, Michael, Risbridger, Gail P. and Exintaris, Betty. 2021. "Oxytocin receptor antagonists as a novel pharmacological agent for reducing smooth muscle tone in the human prostate." Scientific Reports. 11, pp. 1-11. https://doi.org/10.1038/s41598-021-85439-4
Mast Cell‐Derived SAMD14 is a Novel Regulator of the Human Prostate Tumor Microenvironment
Teng, Linda K. H., Pereira, Brooke A., Keerthikumar, Shivakumar, Huang, Cheng, Niranjan, Birunthi, Lee, Sophie N., Richards, Michelle, Schittenhelm, Ralf B., Furic, Luc, Goode, David L., Lawrence, Mitchell G., Taylor, Renea A., Ellem, Stuart J., Risbridger, Gail P. and Lister, Natalie L.. 2021. "Mast Cell‐Derived SAMD14 is a Novel Regulator of the Human Prostate Tumor Microenvironment." Cancers. 13 (6), pp. 1-25. https://doi.org/10.3390/cancers13061237
Contemporary consumer perspectives on prostate cancer survivorship: Fifty voices
Dunn, Jeff, Ralph, Nicholas, Green, Anna, Frydenberg, Mark and Chambers, Suzanne. 2020. "Contemporary consumer perspectives on prostate cancer survivorship: Fifty voices." Psycho-Oncology: journal of the psychological, social and behavioral dimensions of cancer. 29 (3), pp. 557-563. https://doi.org/10.1002/pon.5306
Proteomic Profiling of Human Prostate Cancer-associated Fibroblasts (CAF) Reveals LOXL2-dependent Regulation of the Tumor Microenvironment
Nguyen, Elizabeth V., Pereira, Brooke A., Lawrence, Mitchell G., Ma, Xiuquan, Rebello, Richard J., Chan, Howard, Niranjan, Birunthi, Wu, Yunjian, Ellem, Stuart, Guan, Xiaoqing, Wu, Jianmin, Skhinas, Joanna N., Cox, Thomas R., Risbridger, Gail P., Taylor, Renea A., Lister, Natalie L. and Daly, Roger J.. 2019. "Proteomic Profiling of Human Prostate Cancer-associated Fibroblasts (CAF) Reveals LOXL2-dependent Regulation of the Tumor Microenvironment." Molecular and Cellular Proteomics. 18 (7), pp. 1410-1427. https://doi.org/10.1074/mcp.RA119.001496
Elevated seminal plasma estradiol and epigenetic inactivation of ESR1 and ESR2 is associated with CP/CPPS
Nesheim, Nils, Ellem, Stuart, Dansranjavin, Temuujin, Kagenkotter, Christina, Berg, Elena, Schambeck, Rupert, Schuppe, Hans-Christian, Pilatz, Adrian, Risbridger, Gail, Weidner, Wolfgang, Wagenlehner, Florian and Schagdarsurengin, Undraga. 2018. "Elevated seminal plasma estradiol and epigenetic inactivation of ESR1 and ESR2 is associated with CP/CPPS." Oncotarget. 9 (28), pp. 19623-19639. https://doi.org/10.18632/oncotarget.24714
Antimicrobial and immunomodulatory surface-functionalized electrospun membranes for bone regeneration
Mathew, Asha, Vaquette, Cedryck, Hashimi, Saeed, Rathnayake, Irani, Huygens, Flavia, Hutmacher, Dietmar and Ivanovski, Saso. 2017. "Antimicrobial and immunomodulatory surface-functionalized electrospun membranes for bone regeneration." Advanced Healthcare Materials. 6 (10). https://doi.org/10.1002/adhm.201601345
New Challenges in Psycho-Oncology Research III: A systematic review of psychological interventions for prostate cancer survivors and their partners: clinical and research implications
Chambers, Suzanne K., Hyde, Melissa K., Smith, David P., Hughes, Suzanne, Yuill, Susan, Egger, Sam, O'Connell, Dianne L., Stein, Kevin, Frydenberg, Mark, Wittert, Gary and Dunn, Jeff. 2017. "New Challenges in Psycho-Oncology Research III: A systematic review of psychological interventions for prostate cancer survivors and their partners: clinical and research implications." Psycho-Oncology: journal of the psychological, social and behavioral dimensions of cancer. 26 (7), pp. 873-913. https://doi.org/10.1002/pon.4431
Periodontal tissue graft
Farag, Amro, Mathew, Asha, Vaquette, Cedryck, Hutmacher, Dietmar and Ivanovski, Saso. 2014. Periodontal tissue graft. AU2014903870