Complete Mitochondrial Genome and Phylogenetic Position of Chirolophis wui (Perciformes: Stichaeidae)
Article
Lee, Yong-Suk, Patil, Maheshkumar Prakash, Kim, Jong-Oh, Lee, Yu-Jin, Seo, Yong Bae, Kim, Jin-Koo, Mahale, Kiran R. and Kim, Gun-Do. 2023. "Complete Mitochondrial Genome and Phylogenetic Position of Chirolophis wui (Perciformes: Stichaeidae)." Fishes. 8 (3). https://doi.org/10.3390/fishes8030165
| Article Title | Complete Mitochondrial Genome and Phylogenetic Position of Chirolophis wui (Perciformes: Stichaeidae) |
|---|---|
| ERA Journal ID | 212513 |
| Article Category | Article |
| Authors | Lee, Yong-Suk, Patil, Maheshkumar Prakash, Kim, Jong-Oh, Lee, Yu-Jin, Seo, Yong Bae, Kim, Jin-Koo, Mahale, Kiran R. and Kim, Gun-Do |
| Journal Title | Fishes |
| Journal Citation | 8 (3) |
| Article Number | 165 |
| Number of Pages | 12 |
| Year | 2023 |
| Publisher | MDPI AG |
| Place of Publication | Switzerland |
| ISSN | 2410-3888 |
| Digital Object Identifier (DOI) | https://doi.org/10.3390/fishes8030165 |
| Web Address (URL) | https://www.mdpi.com/2410-3888/8/3/165 |
| Abstract | The complete mitochondrial genome of Chirolophis wui (Wang and Wang, 1935) was sequenced using the Illumina platform. The genome sequence is 16,522 bp in length with 54% A+T content and contains 13 protein coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), and 1 control region (D-loop). The H-strand contains 28 genes (12 PCGs, 14 tRNAs, and 2 rRNAs), whereas the L-strand accommodates 9 genes (ND6 and 8 tRNAs). The nucleotide composition of the mitochondrial genome of C. wui is AT-biased, accounting for 54.0%, with an AT skew value of −0.0556 and a GC skew value of −0.2043. The majority of PCGs utilized the start codon, ATG, while only one gene, COI, utilized the alternative start codon, GTG. Of the 13 PCGs, 6 genes used the termination codon (TAA or TGA), whereas 7 genes used the incomplete termination codon (T or TA). Among the 22 tRNA genes, the tRNA-Leu and tRNA-Ser were found in duplicates. A phylogenetic tree was constructed using 10 complete mitochondrial genome sequences and indicated that C. wui has a very close relationship with C. japonicus and other species in the family Stichaeidae, with a high supporting bootstrap value. This study can provide valuable information for future evolutionary studies on C. wui and Stichaeidae. |
| Keywords | Chirolophis wui; Stichaeidae; mitogenome; next-generation sequencing; phylogenetic analysis |
| ANZSRC Field of Research 2020 | 3107. Microbiology |
| Byline Affiliations | Pukyong National University, Korea |
| School of Engineering |
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