Variation Under Nature: A Sesquicentennial DNA Barcoding Perspective
Article
Ballard, J. William O., Puslednik, Louise, Wolff, Jonci N. and Russell, Richard C.. 2009. "Variation Under Nature: A Sesquicentennial DNA Barcoding Perspective." Chiang Mai Journal of Science. 36 (2), pp. 188-199.
| Article Title | Variation Under Nature: A Sesquicentennial DNA Barcoding Perspective |
|---|---|
| ERA Journal ID | 512 |
| Article Category | Article |
| Authors | Ballard, J. William O., Puslednik, Louise, Wolff, Jonci N. and Russell, Richard C. |
| Journal Title | Chiang Mai Journal of Science |
| Journal Citation | 36 (2), pp. 188-199 |
| Number of Pages | 12 |
| Year | 2009 |
| Publisher | Chiang Mai University |
| ISSN | 0125-2526 |
| 1513-489X | |
| Web Address (URL) | https://www.thaiscience.info/view3.asp?sCode=CMJS&sType=JOURNAL#Year%202009,%20Volume%2036 |
| Abstract | Darwin's classic reference to the "tangled bank" acknowledges the living biodiversity. Traditionally, biodiversity assessment has been the realm of the classical taxonomist. However, times have changed swiftly and now biodiversity may be assessed by high throughput sequencing of short regions of DNA. Commonly this is called DNA "barcoding". In this study, we investigate the genetic diversity within and between four morphological mosquito species that are vectors of medically important viral pathogens. We obtained 627 bp of sequence information from the N-terminus of the mitochondrially encoded cytochrome c oxidase I (COI) gene from 12 Aedes vigilax and 10 Ae. camptorhynchus representing five localities in Australia. We also downloaded GenBank COI sequence data from 18 Ae. albopictus and 14 Ae. aegypti. Maximum likelihood phylogenetic analyses recovered the four distinct morphological species, as well as a distinct lineage within what is currently described as Ae. vigilax. We termed this lineage 'A'. Intra- and interspecific variation quantified for Ae. vigilax and Ae. vigilax lineage 'A' suggest that this morphospecies may include distinct genetic or cryptic species. Ae. aegypti also had high intraspecific variation, however, we concluded that this is most likely due to the large effective population size of this species and its broad distribution through the tropics. Phylogenetic analyses and analyses of genetic diversity at COI suggests that Ae. camptorhynchus and Ae. albopictus are robust species. The results of this study highlight the efficiency, utility and ease of barcoding. The study also documents specific limitations including the importance of taxon sampling and the necessity for this tool to be used in conjunction with additional taxonomic techniques, ecological studies and physiological data. |
| Keywords | barcode; Aedes vigilax; Diversity; Mosquito |
| Contains Sensitive Content | Does not contain sensitive content |
| ANZSRC Field of Research 2020 | 310410. Phylogeny and comparative analysis |
| Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
| Byline Affiliations | University of New South Wales |
| University of Sydney |
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