Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest,USA, by internal transcribed spacer sequences and phylogenetic analysis

Article


Petrisko, Jill E., Pearl, Christopher A., Pilliod, David S., Sheridan, Peter P., Williams, Charles F., Peterson, Charles R. and Bury, R. Bruce. 2008. "Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest,USA, by internal transcribed spacer sequences and phylogenetic analysis." Mycologia. 100 (2), pp. 171-180. https://doi.org/10.3852/mycologia.100.2.171
Article Title

Saprolegniaceae identified on amphibian eggs throughout the Pacific Northwest,USA, by internal transcribed spacer sequences and phylogenetic analysis

ERA Journal ID2502
Article CategoryArticle
AuthorsPetrisko, Jill E. (Author), Pearl, Christopher A. (Author), Pilliod, David S. (Author), Sheridan, Peter P. (Author), Williams, Charles F. (Author), Peterson, Charles R. (Author) and Bury, R. Bruce (Author)
Journal TitleMycologia
Journal Citation100 (2), pp. 171-180
Number of Pages10
Year2008
Place of PublicationLawrence, KS, USA
ISSN0027-5514
1557-2536
Digital Object Identifier (DOI)https://doi.org/10.3852/mycologia.100.2.171
Web Address (URL)http://www.mycologia.org/content/vol100/issue2/
Abstract

We assessed the diversity and phylogeny of Saprolegniaceae on amphibian eggs from the Pacific Northwest, with particular focus on Saprolegnia ferax, a species implicated in high egg mortality. We identified isolates from eggs of six amphibians with the internal transcribed spacer (ITS) and 5.8S gene regions and BLAST of the GenBank database. We identified 68 sequences as Saprolegniaceae and 43 sequences as true fungi from at least nine genera. Our phylogenetic analysis of the Saprolegniaceae included isolates within the genera Saprolegnia, Achlya and Leptolegnia. Our phylogeny grouped S. semihypogyna with Achlya rather than with the Saprolegnia reference sequences. We found only one isolate that grouped closely with S. ferax, and this came from a hatchery-raised salmon (Idaho) that we sampled opportunistically. We had representatives of 7-12 species and three genera of Saprolegniaceae on our amphibian eggs. Further work on the ecological roles of different species of Saprolegniaceae is needed to clarify their potential importance in amphibian egg mortality and potential links to population declines.

Keywordsachlya, amphibian decline, egg, lake, Leptolegnia, oomycete, Saprolegnia ferax, S. semihypogyna
ANZSRC Field of Research 2020310703. Microbial ecology
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Byline AffiliationsIdaho State University, United States
United States Geological Survey, United States
Aldo Leopold Wilderness Research Institute, United States
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