Defense genes and pathways in Fusarium crown rot susceptible and partially-resistant Australian wheat seedlings responding to Fusarium culmorum infection

Paper


Petrisko, Jill E. and Windes, J. M.. 2008. "Defense genes and pathways in Fusarium crown rot susceptible and partially-resistant Australian wheat seedlings responding to Fusarium culmorum infection." APS 2008 Centennial Meeting. Minneapolis, United States 26 - 28 Jul 2008 American Phytopathological Society.
Paper/Presentation Title

Defense genes and pathways in Fusarium crown rot susceptible and partially-resistant Australian wheat seedlings responding to Fusarium culmorum infection

Presentation TypePaper
AuthorsPetrisko, Jill E. (Author) and Windes, J. M. (Author)
Journal or Proceedings TitlePhytopathology: International Journal of the American Phytopathological Society
Journal Citation98 (6), pp. S125-S125
Number of Pages1
Year2008
PublisherAmerican Phytopathological Society
ISSN0031-949X
1943-7684
Web Address (URL) of Paperhttp://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2008.98.6.S9
Conference/EventAPS 2008 Centennial Meeting
Event Details
APS 2008 Centennial Meeting
Event Date
26 to end of 28 Jul 2008
Event Location
Minneapolis, United States
Abstract

Fusarium crown rot causes considerable losses to wheat production in parts of the United States and Australia. Australian wheat germplasm has been the best source of resistance to Fusarium crown rot caused by F. pseudograminearum. In order to understand the mechanism of resistance to Fusarium crown rot caused by F. culmorum, a transcriptional study using the Affymetrix wheat chip was done to compare the partially-resistant Australian line '2-49' to the susceptible Australian wheat variety ‘Puseas’ at ten days post-inoculation. Genes determined to be significantly different in expression levels were identified in four categories: 2-49 inoculated versus 2-49 non-inoculated, Puseas inoculated versus Puseas non-inoculated, 2-49 inoculated versus Puseas inoculated and 2-49 non-inoculated versus Puseas non-inoculated. Five candidate genes, oxalate oxidase, chitinase 1, glycosyltransferase, WIR1, and a stress-related protein were selected for further expression characterization at one, five, and ten days post-inoculation using quantitative real-time RT-PCR. The quantitative real-time RT-PCR results confirmed the microarray results and showed that differential expression in these genes was due to differences in genetic background, inoculation response, or the interaction of both factors. These genes are being investigated for their roles in the resistance response of 2-49 using a viral-induced gene silencing (VIGS) study and pathways analysis. An understanding of how 2-49 prevents F. culmorum spread throughout the seedling will greatly improve our understanding of resistance to fungal invasion in wheat.

Keywordswheat; crown rot
ANZSRC Field of Research 2020310805. Plant pathology
Public Notes

This is an abstract of a paper presented at the 100th Annual Meeting of the American-Phytopathological-Society
Minneapolis, MN, JUL 26-30, 2008, Amer Phytopathol Soc.

Byline AffiliationsCentre for Systems Biology
University of Idaho, United States
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