Extremely radiation-resistant mutants of a halophilic archaeon with increased single-stranded DNA-binding protein (RPA) gene expression

Article


DeVeaux, L. C., Muller, J. A., Smith, J., Petrisko, Jill E., Wells, D. P. and DasSarma, S.. 2007. "Extremely radiation-resistant mutants of a halophilic archaeon with increased single-stranded DNA-binding protein (RPA) gene expression." Radiation Research. 168 (4), pp. 507-514. https://doi.org/10.1667/RR0935.1
Article Title

Extremely radiation-resistant mutants of a halophilic archaeon with increased single-stranded DNA-binding protein (RPA) gene expression

ERA Journal ID2117
Article CategoryArticle
AuthorsDeVeaux, L. C. (Author), Muller, J. A. (Author), Smith, J. (Author), Petrisko, Jill E. (Author), Wells, D. P. (Author) and DasSarma, S. (Author)
Journal TitleRadiation Research
Journal Citation168 (4), pp. 507-514
Number of Pages8
Year2007
Place of PublicationNew York, USA
ISSN0033-7587
1938-5404
Digital Object Identifier (DOI)https://doi.org/10.1667/RR0935.1
Abstract

Extremely halophilic archaea are highly resistant to multiple stressors, including radiation, desiccation and salinity. To study the basis of stress resistance and determine the maximum tolerance to ionizing radiation, we exposed cultures of the model halophile Halobacterium sp. NRC-1 to four cycles of irradiation with high doses of 18-20 MeV electrons. Two independently obtained mutants displayed an LD50 > 11 kGy, which is higher than the LD50 of the extremely radiation-resistant bacterium Deinococcus radiodurans. Whole-genome transcriptome analysis comparing the mutants to the parental wild-type strain revealed up-regulation of an operon containing two single-stranded DNA-binding protein (RPA) genes, VNG2160 (rfa3) and VNG2162, and a third gene of unknown function, VNG2163. The putative transcription start site for the rfa3 operon was mapped ∼40 bp upstream of the ATG start codon, and a classical TATA-box motif was found centered about 25 bp further upstream. We propose that RPA facilitates DNA repair machinery and/or protects repair intermediates to maximize the ionizing radiation resistance of this archaeon.

Keywordsprotein rfa3; single stranded DNA binding protein; unclassified drug; replication protein A, Archaea; Bacteria (microorganisms); Deinococcus radiodurans; Halobacterium sp. NRC-1, Base Sequence; DNA Repair; Gene Expression Regulation
ANZSRC Field of Research 2020310701. Bacteriology
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Byline AffiliationsIdaho State University, United States
University of Maryland, United States
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