Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice

Article


Wang, Yangwenjie, Xiang, Yang, Wang, Ruiqi, Li, Xiangning, Wang, Jianxiong, Yu, Siwang and Zhang, Ying. 2022. "Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice." Food Science and Human Wellness. 11 (5), pp. 1355-1361. https://doi.org/10.1016/j.fshw.2022.04.035
Article Title

Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice

ERA Journal ID212526
Article CategoryArticle
AuthorsWang, Yangwenjie, Xiang, Yang, Wang, Ruiqi, Li, Xiangning, Wang, Jianxiong, Yu, Siwang and Zhang, Ying
Journal TitleFood Science and Human Wellness
Journal Citation11 (5), pp. 1355-1361
Number of Pages7
Year2022
PublisherKeAi Publishing Communications Ltd.
ISSN2213-4530
Digital Object Identifier (DOI)https://doi.org/10.1016/j.fshw.2022.04.035
Web Address (URL)https://www.sciencedirect.com/science/article/pii/S2213453022000738
Abstract

Nuclear factor erythroid-derived 2-like 2 (Nrf2) is the master regulator of antioxidant defenses. High-intensity interval training (HIIT) has been proposed as a time-efficient training program and has become a substantial component of modern training program. In the present study, we evaluated the effects of sulforaphane (SFN), a dietary isothiocyanate derived from cruciferous vegetables and a potent Nrf2 activator, on Nrf2-mediated antioxidant defense responses of skeletal muscle induced by exhaustive exercise in HIIT mice. Male C57BL/6J mice were randomly allocated into control group, HIIT group, and HIIT pretreated with SFN (HIIT+SFN) group. On the third day after completion of a 6-weeks HIIT protocol, an exhaustive treadmill test was conducted in all mice. Mice were intraperitoneally injected with SFN (HIIT+SFN group) or PBS (HIIT and control mice) 4 times in 3 days prior to the exhaustive treadmill test. The results indicated that the 6-weeks HIIT protocol did not increase the antioxidative capacity of skeletal muscle during exhaustive exercise. Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice.

KeywordsSulforaphane; Nrf2 (nuclear factor erythroid-derived 2-like 2) ; Skeletal muscle; Antioxidant; HIIT (high-intensity interval training); Mice
Byline AffiliationsBeijing Sport University, China
Yan’an University, China
University of Southern Queensland
Peking University, China
Permalink -

https://research.usq.edu.au/item/z02xy/sulforaphane-enhances-nrf2-mediated-antioxidant-responses-of-skeletal-muscle-induced-by-exhaustive-exercise-in-hiit-mice

Download files


Published Version
1-s2.0-S2213453022000738-main.pdf
License: CC BY-NC-ND 4.0
File access level: Anyone

  • 21
    total views
  • 11
    total downloads
  • 2
    views this month
  • 2
    downloads this month

Export as

Related outputs

Effects of Six Weeks of Hypoxia Exposure on Hepatic Fatty Acid Metabolism in ApoE Knockout Mice Fed a High-Fat Diet
Wang, Yangwenjie, Lavier, Jessica, Hua, Weicheng, Gong, Lijing, Wei, Hao, Wang, Jianxiong, Pellegrin, Maxime, Millet, Grégoire P. and Zhang, Ying. 2022. "Effects of Six Weeks of Hypoxia Exposure on Hepatic Fatty Acid Metabolism in ApoE Knockout Mice Fed a High-Fat Diet." Life. 12 (10). https://doi.org/10.3390/life12101535
Skeletal-Muscle-Specific Overexpression of Chrono Leads to Disruption of Glucose Metabolism and Exercise Capacity
He, Shiyi, Yan, Lu, Zhu, Rongxin, Wei, Hao, Wang, Jianxiong, Zheng, Lan and Zhang, Ying. 2022. "Skeletal-Muscle-Specific Overexpression of Chrono Leads to Disruption of Glucose Metabolism and Exercise Capacity." Life. 12 (8). https://doi.org/10.3390/life12081233
High-intensity interval training and moderate-intensity continuous training attenuate oxidative damage and promote myokine response in the skeletal muscle of ApoE KO mice on high-fat diet
Wang, Linjia, Lavier, Jessica, Hua, Weicheng, Wang, Yangwenjie, Gong, Lijing, Wei, Hao, Wang, Jianxiong, Pellegrin, Maxime, Millet, Gregoire P. and Zhang, Ying. 2021. "High-intensity interval training and moderate-intensity continuous training attenuate oxidative damage and promote myokine response in the skeletal muscle of ApoE KO mice on high-fat diet." Antioxidants. 10 (7). https://doi.org/10.3390/antiox10070992
Nrf2 Activation Enhances Muscular MCT1 Expression and Hypoxic Exercise Capacity
Wang, Linjia, Zhu, Rongxin, Wang, Jiahui, Yu, Siwang, Wang, Jianxiong and Zhang, Ying. 2020. "Nrf2 Activation Enhances Muscular MCT1 Expression and Hypoxic Exercise Capacity." Medicine and Science in Sports and Exercise. 52 (8), pp. 1719-1728. https://doi.org/10.1249/MSS.0000000000002312
Hypoxia preconditioning promotes endurance exercise capacity of mice by activating skeletal muscle Nrf2
Wang, Linjia, Yang, Simin, Yan, Lu, Wei, Hao, Wang, Jianxiong, Yu, Siwang, Kong, Ah-Ng Tony and Zhang, Ying. 2019. "Hypoxia preconditioning promotes endurance exercise capacity of mice by activating skeletal muscle Nrf2." Journal of Applied Physiology. 127, pp. 1267-1277. https://doi.org/10.1152/japplphysiol.00347.2019
Effects of acute hypoxia exposure with different durations on activation of Nrf2-ARE pathway in mouse skeletal muscle
Ji, Weixiu, Wang, Linjia, He, Shiyi, Yan, Lu, Li, Tieying, Wang, Jianxiong, Kong, Ah-Ng Tony, Yu, Siwang and Zhang, Ying. 2018. "Effects of acute hypoxia exposure with different durations on activation of Nrf2-ARE pathway in mouse skeletal muscle." PLoS One. 13 (12), pp. 1-15. https://doi.org/10.1371/journal.pone.0208474
Naming and outline of Dothideomycetes–2014 including proposals for the protection or suppression of generic names
Wijayawardene, Nalin N., Crous, Pedro W., Kirk, Paul M., Hawksworth, David L., Boonmee, Saranyaphat, Braun, Uwe, Dai, Dong Qin, D'souza, Melvina J., Diederich, Paul, Dissanayake, Asha, Phookamsak, Rungtiwa, Hyde, Kevin D., Doilom, Mingkhuan, Hongsanan, Singang, Jayawardena, Ruvishika, Liu, Jian-Kui, Manamgoda, Dimuthu S., Thambugala, Kasun M., Wanasinghe, Dhanushka N., ..., Jaklitsch, Walter M.. 2014. "Naming and outline of Dothideomycetes–2014 including proposals for the protection or suppression of generic names." Fungal Diversity. 69 (1), pp. 1-55. https://doi.org/10.1007/s13225-014-0309-2