Nrf2 Activation Enhances Muscular MCT1 Expression and Hypoxic Exercise Capacity

Article


Wang, Linjia, Zhu, Rongxin, Wang, Jiahui, Yu, Siwang, Wang, Jianxiong and Zhang, Ying. 2020. "Nrf2 Activation Enhances Muscular MCT1 Expression and Hypoxic Exercise Capacity." Medicine and Science in Sports and Exercise. 52 (8), pp. 1719-1728. https://doi.org/10.1249/MSS.0000000000002312
Article Title

Nrf2 Activation Enhances Muscular MCT1 Expression and Hypoxic Exercise Capacity

ERA Journal ID9788
Article CategoryArticle
AuthorsWang, Linjia (Author), Zhu, Rongxin (Author), Wang, Jiahui (Author), Yu, Siwang (Author), Wang, Jianxiong (Author) and Zhang, Ying (Author)
Journal TitleMedicine and Science in Sports and Exercise
Journal Citation52 (8), pp. 1719-1728
Number of Pages10
Year2020
PublisherLippincott Williams & Wilkins
Place of PublicationUnited States
ISSN0195-9131
1530-0315
Digital Object Identifier (DOI)https://doi.org/10.1249/MSS.0000000000002312
Web Address (URL)https://journals.lww.com/acsm-msse/Fulltext/2020/08000/Nrf2_Activation_Enhances_Muscular_MCT1_Expression.9.aspx
Abstract

Introduction
Skeletal muscle is the major producing and metabolizing site of lactic acid. A family of monocarboxylate transporter (MCT) proteins, especially MCT1 and MCT4, are involved in the lactate–pyruvate exchange and metabolism. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a pivotal coordinator of antioxidant response and energy metabolism, and has been reported to associate with the physiological functions of the skeletal muscle.

Methods
In this study, C57BL/6 J mice were administrated with an Nrf2 activator, sulforaphane (SFN) before taking incremental treadmill exercise to exhaustion under hypoxia; then the effects of SFN on exercise endurance and molecular/biochemical makers of the skeletal muscle were evaluated.

Results
The results indicated that SFN pretreatment enhanced the exercise endurance under hypoxia. SFN not only increased the expressions of antioxidant genes and activity of antioxidant enzymes, but also significantly increased the mRNA and protein levels of MCT1 and CD147, but not MCT4. Moreover, the expressions of LDH-B and LDH activity of converting lactate into pyruvate, as well as citrate synthase activity were significantly higher, whereas the LDH activity of converting pyruvate into lactate and blood lactate level were remarkably lower in the SFN-exercise mice than those of the phosphate-buffered saline–exercise group. Furthermore, Atf3Δzip2 (the alternatively spliced isoform of activating transcription factor-3) mRNA was increased by the exercise and further potentiated by SFN.

Conclusion
These results show, for the first time, that SFN increases MCT1 expression in the skeletal muscle under acute hypoxic exercise and suggest that Nrf2 activation is a promising strategy to enhance exercise performance under hypoxia.

KeywordsMonocarboxylate Transporter 1; Lactate; Nrf2; Skeletal Muscle; Hypoxic Exercise; Sulforaphane
ANZSRC Field of Research 2020420702. Exercise physiology
Byline AffiliationsBeijing Sport University, China
Shanghai Research Institute of Sports Science, China
Peking University, China
Faculty of Health, Engineering and Sciences
Institution of OriginUniversity of Southern Queensland
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