Effects of different exercise durations on Keap1-Nrf2-ARE pathway activation in mouse skeletal muscle

Article

Li, Tieying, He, Sheyi, Liu, Sixue, Kong, Zhenxing, Wang, Jianxiong and Zhang, Ying. 2015. "Effects of different exercise durations on Keap1-Nrf2-ARE pathway activation in mouse skeletal muscle." Free Radical Research. 49 (10), pp. 1269-1274. https://doi.org/10.3109/10715762.2015.1066784
Article Title

Effects of different exercise durations on Keap1-Nrf2-ARE pathway activation in mouse skeletal muscle

ERA Journal ID2219
Article CategoryArticle
AuthorsLi, Tieying (Author), He, Sheyi (Author), Liu, Sixue (Author), Kong, Zhenxing (Author), Wang, Jianxiong (Author) and Zhang, Ying (Author)
Journal TitleFree Radical Research
Journal Citation49 (10), pp. 1269-1274
Number of Pages6
Year2015
PublisherTaylor & Francis
Place of PublicationSwitzerland
ISSN1029-2470
1071-5762
Digital Object Identifier (DOI)https://doi.org/10.3109/10715762.2015.1066784
Web Address (URL)http://www.tandfonline.com/doi/full/10.3109/10715762.2015.1066784
Abstract

The purpose of this study was to investigate the effects of acute exercise stress on the nuclear factor-erythroid2 p45-related factor 2 (Nrf2)/antioxidant response element (ARE) transactivation, Kelch-like ECH-associated protein 1(Keap1) cytosolic protein and Nrf2 nucleoprotein expressions, Nrf2 target genes mRNA expressions, and GSH/GSSG ratio level; with a particular focus on the changes of Keap1-Nrf2-ARE pathway activation following different durations of exercise. Wild-type mice (C57BL/6J, two months old) were separated into one-hour and six-hour treadmill running groups, as well as a non-exercise control group (n=10 in each group). Measurements of Nrf2/ARE transactivation, Nrf2 nucleoprotein expressions, Keap1 cytosolic protein expression, Nrf2 target genes' mRNA expressions (superoxide dismutase-1 (SOD1), superoxide dismutase-2 (SOD2), γ-glutamyl cysteine ligase-modulatory (GCLm), γ-glutamylcysteine ligase-catalytic (GCLc), glutathione reductase (GR), glutathione peroxydase-1 (Gpx1), catalase (CAT) and hemoxygenase-1 (Ho-1)), and glutathione redox (GSH/GSSG) ratio were carried out immediately after exercise. The results showed significant increases in Keap1-Nrf2-ARE pathway activation and the mRNA expressions of six measured enzymes in skeletal muscle after six hours of exercise; while in the one-hour exercise group, there was no change in Keap1-Nrf2-ARE pathway activation and only two enzymes' mRNA expressions were increased. It is suggested that the changes in Keap1-Nrf2-ARE pathway activation and its target genes' mRNA expressions were dependent on the exercise duration, with longer duration associated with higher responses.

KeywordsNrf2 transactivation, oxidative stress, acute exercise, antioxidant enzyme
ANZSRC Field of Research 2020420702. Exercise physiology
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Byline AffiliationsBeijing Sport University, China
Ministry of Education, China
School of Health and Wellbeing
Institution of OriginUniversity of Southern Queensland
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