Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface

Article

Seymour, Lisa M., Jenkins, Cheryl, Deutscher, Ania T., Raymond, Benjamin B. A., Padula, Matthew P., Tacchi, Jessica L., Bogema, Daniel R., Eamens, Graeme J., Woolley, Lauren K., Dixon, Nicholas E., Walker, Mark J. and Djordjevic, Steven P.. 2012. "Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface." Cellular Microbiology. 14 (1), pp. 81-94. https://doi.org/10.1111/j.1462-5822.2011.01702.x
Article Title

Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface

ERA Journal ID2526
Article CategoryArticle
AuthorsSeymour, Lisa M. (Author), Jenkins, Cheryl (Author), Deutscher, Ania T. (Author), Raymond, Benjamin B. A. (Author), Padula, Matthew P. (Author), Tacchi, Jessica L. (Author), Bogema, Daniel R. (Author), Eamens, Graeme J. (Author), Woolley, Lauren K. (Author), Dixon, Nicholas E. (Author), Walker, Mark J. (Author) and Djordjevic, Steven P. (Author)
Journal TitleCellular Microbiology
Journal Citation14 (1), pp. 81-94
Number of Pages14
Year2012
Place of PublicationChichester, West Sussex. United Kingdom
ISSN1462-5814
1462-5822
Digital Object Identifier (DOI)https://doi.org/10.1111/j.1462-5822.2011.01702.x
Abstract

Mycoplasma hyopneumoniae is a major, economically damaging respiratory pathogen. Although M. hyopneumoniae cells bind plasminogen, the identification of plasminogen-binding surface proteins and the biological ramifications of acquiring plasminogen requires further investigation.
mhp182 encodes a highly expressed 102 kDa protein (P102) that undergoes proteolytic processing to generate surface-located N-terminal 60 kDa
(P60) and C-terminal 42 kDa (P42) proteins of unknown function. We show that recombinant P102 (rP102) binds plasminogen at physiologically relevant concentrations (KD ~ 76 nM) increasing the susceptibility of plasmin(ogen) to activation by tissue-specific plasminogen activator (tPA).
Recombinant proteins constructed to mimic P60 (rP60) and P42 (rP42) also bound plasminogen at physiologically significant levels. M. hyopneumoniae
surface-bound plasminogen was activated by tPA and is able to degrade fibrinogen, demonstrating the biological functionality of M. hyopneumoniae-bound plasmin(ogen) upon activation. Plasmin(ogen) was readily detected in porcine ciliated airways and plasmin levels were consistently higher in bronchoalveolar lavage fluid from M. hyopneumoniae-infected animals. Additionally, rP102 and rP42 bind fibronectin with KDs of 26 and 33 nM respectively and recombinant P102 proteins promote adherence to porcine kidney epithelial-like cells. The multifunctional binding ability of P102 and activation of M. hyopneumoniae-sequestered plasmin(ogen) by an exogenous activator suggests P102 plays an important role in virulence.

Keywordsbacterial protein; fibronectin; p102 protein; plasmin; plasminogen; protein p42; protein p60; recombinant protein; tissue plasminogen activator; bacterial virulence; cell adhesion
ANZSRC Field of Research 2020300999. Veterinary sciences not elsewhere classified
Public Notes

Files associated with this item cannot be displayed due to copyright restrictions.

Byline AffiliationsUniversity of Wollongong
Elizabeth Macarthur Agricultural Institute, Australia
University of Technology Sydney
Department of Primary Industries, New South Wales
University of Queensland
Institution of OriginUniversity of Southern Queensland
Permalink -

https://research.usq.edu.au/item/q25yz/mhp182-p102-binds-fibronectin-and-contributes-to-the-recruitment-of-plasmin-ogen-to-the-mycoplasma-hyopneumoniae-cell-surface

Log in to edit
  • 1720
    total views
  • 15
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Correction: Alhamami et al. First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia. Microorganisms 2021, 9, 1322
Alhamami, Tamara, Chowdhury, Piklu Roy, Gomes, Nancy, Carr, Mandi, Veltman, Tania, Khazandi, Manouchehr, Mollinger, Joanne, Deutscher, Ania T., Turni, Conny, Mahdi, Layla, Venter, Henrietta, Abraham, Sam, Djordjevic, Steven P. and Trott, D.. 2022. "Correction: Alhamami et al. First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia. Microorganisms 2021, 9, 1322." Microorganisms. 10 (4), pp. 1-2. https://doi.org/10.3390/microorganisms10040825
First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
Alhamami, Tamara, Chowdhury, Piklu Roy, Gomes, Nancy, Carr, Mandi, Veltman, Tania, Khazandi, Manouchehr, Mollinger, Joanne, Deutscher, Ania T., Turni, Conny, Mahdi, Layla, Venter, Henrietta, Abraham, Sam, Djordjevic, Steven P. and Trott, Darren J.. 2021. "First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia." Microorganisms. 9 (6), pp. 1-17. https://doi.org/10.3390/microorganisms9061322
Anti-inflammatory activity and structure-activity relationships of brominated indoles from a marine mollusc
Ahmad, Tarek B., Rudd, David, Smith, Joshua, Kotiw, Michael, Mouatt, Peter, Seymour, Lisa M., Liu, Lei and Benkendorff, Kirsten. 2017. "Anti-inflammatory activity and structure-activity relationships of brominated indoles from a marine mollusc." Marine Drugs. 15 (5), pp. 1-19. https://doi.org/10.3390/md15050133
Proteolytic processing of the cilium adhesin MHJ_0194 (P123J ) in Mycoplasma hyopneumoniae generates a functionally diverse array of cleavage fragments that bind multiple host molecules
Raymond, Benjamin B. A., Jenkins, Cheryl, Seymour, Lisa M., Tacchi, Jessica L., Widjaja, Michael, Jarocki, Veronica M., Deutscher, Ania T., Turnbull, Lynne, Whitchurch, Cynthia B., Padula, Matthew P. and Djordjevic, Steven P.. 2014. "Proteolytic processing of the cilium adhesin MHJ_0194 (P123J ) in Mycoplasma hyopneumoniae generates a functionally diverse array of cleavage fragments that bind multiple host molecules." Cellular Microbiology. 17 (3), pp. 425-444. https://doi.org/10.1111/cmi.12377
Mutual exclusivity of hyaluronan and hyaluronidase in invasive group A Streptococcus
Henningham, Anna, Yamaguchi, Masaya, Aziz, Ramy K., Kuipers, Kirsten, Buffalo, Cosmo Z., Dahesh, Samira, Choudhury, Biswa, Van Vleet, Jeremy, Yamaguchi, Yuka, Seymour, Lisa M., Ben Zakour, Nouri L., He, Lingjun, Smith, Helen V., Grimwood, Keith, Beatson, Scott A., Ghosh, Partho, Walker, Mark J., Nizet, Victor and Cole, Jason N.. 2014. "Mutual exclusivity of hyaluronan and hyaluronidase in invasive group A Streptococcus." Journal of Biological Chemistry. 289 (46), pp. 32303-32315. https://doi.org/10.1074/jbc.M114.602847
Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae
Seymour, Lisa M., Falconer, Linda, Deutscher, Ania T., Minion, F. Chris, Padula, Matthew P., Dixon, Nicholas E., Djordjevic, Steven P. and Walker, Mark J.. 2011. "Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae." Journal of Biological Chemistry. 286 (12), pp. 10097-10104. https://doi.org/10.1074/jbc.M110.208140
Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which mycoplasma hyopneumoniae regulates surface topography
Bogema, Daniel R., Deutscher, Ania T., Woolley, Lauren K., Seymour, Lisa M., Raymond, Benjamin B. A., Tacchi, Jessica L., Padula, Matthew P., Dixon, Nicholas E., Minion, F. Chris, Jenkins, Cheryl, Walker, Mark J. and Djordjevic, Steven P.. 2012. "Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which mycoplasma hyopneumoniae regulates surface topography." mBio. 3 (2), pp. 1-12. https://doi.org/10.1128/mBio.00282-11
Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia
Deutscher, Ania T., Jenkins, Cheryl, Minion, F. Chris, Seymour, Lisa M., Padula, Matthew P., Dixon, Nicholas E., Walker, Mark J. and Djordjevic, Steven P.. 2010. "Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia." Molecular Microbiology. 78 (2), pp. 444-458. https://doi.org/10.1111/j.1365-2958.2010.07345.x
A processed multidomain hyopneumoniae adhesin binds fibronectin, plasminogen and swine respiratory cilia
Seymour, Lisa M., Deutscher, Ania T., Jenkins, Cheryl, Kuit, Tracey A., Falconer, Linda, Minion, F. Chris, Crossett, Ben, Padula, Matthew, Dixon, Nicholas E., Djordjevic, Steven P. and Walker, Mark J.. 2010. "A processed multidomain hyopneumoniae adhesin binds fibronectin, plasminogen and swine respiratory cilia." Journal of Biological Chemistry. 285 (44), pp. 33971-33978. https://doi.org/10.1074/jbc.M110.104463
The globally disseminated M1T1 clone of Group A streptococcus evades autophagy for intracellular replication
Barnett, Timothy C., Liebl, David, Seymour, Lisa M., Gillen, Christine M., Yan Lim, Jin, LaRock, Christopher N., Davies, Mark R., Schulz, Benjamin L., Nizet, Victor, Teasdale, Rohan D. and Walker, Mark J.. 2013. "The globally disseminated M1T1 clone of Group A streptococcus evades autophagy for intracellular replication." Cell Host and Microbe. 14 (6), pp. 675-682. https://doi.org/10.1016/j.chom.2013.11.003