Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae

Article

Seymour, Lisa M., Falconer, Linda, Deutscher, Ania T., Minion, F. Chris, Padula, Matthew P., Dixon, Nicholas E., Djordjevic, Steven P. and Walker, Mark J.. 2011. "Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae." Journal of Biological Chemistry. 286 (12), pp. 10097-10104. https://doi.org/10.1074/jbc.M110.208140
Article Title

Mhp107 is a member of the multifunctional adhesin family
of Mycoplasma hyopneumoniae

ERA Journal ID2230
Article CategoryArticle
AuthorsSeymour, Lisa M. (Author), Falconer, Linda (Author), Deutscher, Ania T. (Author), Minion, F. Chris (Author), Padula, Matthew P. (Author), Dixon, Nicholas E. (Author), Djordjevic, Steven P. (Author) and Walker, Mark J. (Author)
Journal TitleJournal of Biological Chemistry
Journal Citation286 (12), pp. 10097-10104
Number of Pages8
Year2011
Place of PublicationRockville, MD. United States
ISSN0021-9258
1083-351X
Digital Object Identifier (DOI)https://doi.org/10.1074/jbc.M110.208140
Web Address (URL)http://www.jbc.org/content/286/12/10097
Abstract

Mycoplasma hyopneumoniae is the causative pathogen of porcine enzootic pneumonia, an economically significant disease that disrupts the mucociliary escalator in the swine respiratory tract. Expression of Mhp107, a P97 paralog encoded by the gene mhp107, was confirmed using ESI-MS/MS. To investigate the function of Mhp107, three recombinant proteins, F1Mhp107, F2Mhp107, and F3Mhp107, spanning the N-terminal, central, and C-terminal regions of Mhp107 were constructed. Colonization of swine by M. hyopneumoniae requires adherenceof the bacterium to ciliated cells of the respiratory tract.
Recent studies have identified a number of M. hyopneumoniae adhesins that bind heparin, fibronectin, and plasminogen. F1Mhp107 was found to bind porcine heparin (KD ~90 nM) in a dose-dependent and saturable manner, whereas F3Mhp107 bound fibronectin (KD~180 nM) at physiologically relevant concentrations. F1Mhp107 also bound porcine plasminogen (KD24~n a dose-dependent and physiologically relevant manner. Microspheres coated with F3Mhp107 mediate adherence to porcine kidney epithelial-like (PK15) cells, and all three recombinant proteins (F1Mhp107-F3Mhp107) bound swine respiratory cilia. Together, these findings indicate that Mhp107 is a member of the multifunctional M. hyopneumoniae adhesin family of surface proteins and contributes to both adherence to the host and pathogenesis.

Keywordsadhesin; fibronectin; heparin; plasminogen; protein Mhp107; recombinant protein; ciliated cells; amino terminal sequence; animal cell; animal experiment; bacterial colonization; bacterium adherence; carboxy terminal sequence; ciliated epithelium; fibronectins
ANZSRC Field of Research 2020300304. Animal protection (incl. pests and pathogens)
310701. Bacteriology
300999. Veterinary sciences not elsewhere classified
Public Notes

Files associated with this item cannot be displayed due to copyright restrictions.

Byline AffiliationsUniversity of Wollongong
Elizabeth Macarthur Agricultural Institute, Australia
Iowa State University, United States
University of Technology Sydney
University of Queensland
Institution of OriginUniversity of Southern Queensland
Funding source
Australian Research Council (ARC)
Grant ID
LP776771
Permalink -

https://research.usq.edu.au/item/q25z0/mhp107-is-a-member-of-the-multifunctional-adhesin-family-of-mycoplasma-hyopneumoniae

Log in to edit
  • 1688
    total views
  • 7
    total downloads
  • 0
    views this month
  • 0
    downloads this month

Export as

Related outputs

Correction: Alhamami et al. First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia. Microorganisms 2021, 9, 1322
Alhamami, Tamara, Chowdhury, Piklu Roy, Gomes, Nancy, Carr, Mandi, Veltman, Tania, Khazandi, Manouchehr, Mollinger, Joanne, Deutscher, Ania T., Turni, Conny, Mahdi, Layla, Venter, Henrietta, Abraham, Sam, Djordjevic, Steven P. and Trott, D.. 2022. "Correction: Alhamami et al. First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia. Microorganisms 2021, 9, 1322." Microorganisms. 10 (4), pp. 1-2. https://doi.org/10.3390/microorganisms10040825
First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia
Alhamami, Tamara, Chowdhury, Piklu Roy, Gomes, Nancy, Carr, Mandi, Veltman, Tania, Khazandi, Manouchehr, Mollinger, Joanne, Deutscher, Ania T., Turni, Conny, Mahdi, Layla, Venter, Henrietta, Abraham, Sam, Djordjevic, Steven P. and Trott, Darren J.. 2021. "First Emergence of Resistance to Macrolides and Tetracycline Identified in Mannheimia haemolytica and Pasteurella multocida Isolates from Beef Feedlots in Australia." Microorganisms. 9 (6), pp. 1-17. https://doi.org/10.3390/microorganisms9061322
Anti-inflammatory activity and structure-activity relationships of brominated indoles from a marine mollusc
Ahmad, Tarek B., Rudd, David, Smith, Joshua, Kotiw, Michael, Mouatt, Peter, Seymour, Lisa M., Liu, Lei and Benkendorff, Kirsten. 2017. "Anti-inflammatory activity and structure-activity relationships of brominated indoles from a marine mollusc." Marine Drugs. 15 (5), pp. 1-19. https://doi.org/10.3390/md15050133
Proteolytic processing of the cilium adhesin MHJ_0194 (P123J ) in Mycoplasma hyopneumoniae generates a functionally diverse array of cleavage fragments that bind multiple host molecules
Raymond, Benjamin B. A., Jenkins, Cheryl, Seymour, Lisa M., Tacchi, Jessica L., Widjaja, Michael, Jarocki, Veronica M., Deutscher, Ania T., Turnbull, Lynne, Whitchurch, Cynthia B., Padula, Matthew P. and Djordjevic, Steven P.. 2014. "Proteolytic processing of the cilium adhesin MHJ_0194 (P123J ) in Mycoplasma hyopneumoniae generates a functionally diverse array of cleavage fragments that bind multiple host molecules." Cellular Microbiology. 17 (3), pp. 425-444. https://doi.org/10.1111/cmi.12377
Mutual exclusivity of hyaluronan and hyaluronidase in invasive group A Streptococcus
Henningham, Anna, Yamaguchi, Masaya, Aziz, Ramy K., Kuipers, Kirsten, Buffalo, Cosmo Z., Dahesh, Samira, Choudhury, Biswa, Van Vleet, Jeremy, Yamaguchi, Yuka, Seymour, Lisa M., Ben Zakour, Nouri L., He, Lingjun, Smith, Helen V., Grimwood, Keith, Beatson, Scott A., Ghosh, Partho, Walker, Mark J., Nizet, Victor and Cole, Jason N.. 2014. "Mutual exclusivity of hyaluronan and hyaluronidase in invasive group A Streptococcus." Journal of Biological Chemistry. 289 (46), pp. 32303-32315. https://doi.org/10.1074/jbc.M114.602847
Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface
Seymour, Lisa M., Jenkins, Cheryl, Deutscher, Ania T., Raymond, Benjamin B. A., Padula, Matthew P., Tacchi, Jessica L., Bogema, Daniel R., Eamens, Graeme J., Woolley, Lauren K., Dixon, Nicholas E., Walker, Mark J. and Djordjevic, Steven P.. 2012. "Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface." Cellular Microbiology. 14 (1), pp. 81-94. https://doi.org/10.1111/j.1462-5822.2011.01702.x
Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which mycoplasma hyopneumoniae regulates surface topography
Bogema, Daniel R., Deutscher, Ania T., Woolley, Lauren K., Seymour, Lisa M., Raymond, Benjamin B. A., Tacchi, Jessica L., Padula, Matthew P., Dixon, Nicholas E., Minion, F. Chris, Jenkins, Cheryl, Walker, Mark J. and Djordjevic, Steven P.. 2012. "Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which mycoplasma hyopneumoniae regulates surface topography." mBio. 3 (2), pp. 1-12. https://doi.org/10.1128/mBio.00282-11
Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia
Deutscher, Ania T., Jenkins, Cheryl, Minion, F. Chris, Seymour, Lisa M., Padula, Matthew P., Dixon, Nicholas E., Walker, Mark J. and Djordjevic, Steven P.. 2010. "Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia." Molecular Microbiology. 78 (2), pp. 444-458. https://doi.org/10.1111/j.1365-2958.2010.07345.x
A processed multidomain hyopneumoniae adhesin binds fibronectin, plasminogen and swine respiratory cilia
Seymour, Lisa M., Deutscher, Ania T., Jenkins, Cheryl, Kuit, Tracey A., Falconer, Linda, Minion, F. Chris, Crossett, Ben, Padula, Matthew, Dixon, Nicholas E., Djordjevic, Steven P. and Walker, Mark J.. 2010. "A processed multidomain hyopneumoniae adhesin binds fibronectin, plasminogen and swine respiratory cilia." Journal of Biological Chemistry. 285 (44), pp. 33971-33978. https://doi.org/10.1074/jbc.M110.104463
The globally disseminated M1T1 clone of Group A streptococcus evades autophagy for intracellular replication
Barnett, Timothy C., Liebl, David, Seymour, Lisa M., Gillen, Christine M., Yan Lim, Jin, LaRock, Christopher N., Davies, Mark R., Schulz, Benjamin L., Nizet, Victor, Teasdale, Rohan D. and Walker, Mark J.. 2013. "The globally disseminated M1T1 clone of Group A streptococcus evades autophagy for intracellular replication." Cell Host and Microbe. 14 (6), pp. 675-682. https://doi.org/10.1016/j.chom.2013.11.003