Novel diagnostic method for ratoon stunting disease: development and implications for RSD management
Paper
Paper/Presentation Title | Novel diagnostic method for ratoon stunting disease: development and implications for RSD management |
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Presentation Type | Paper |
Authors | Young, Anthony J. (Author), Nock, Catherine J. (Author), Martin, Aimee (Author) and Ensbey, Mark (Author) |
Editors | Bruce, R. C. |
Journal or Proceedings Title | Proceedings of the 36th Annual Conference of the Australian Society of Sugar Cane Technologists (ASSCT 2014) |
ERA Conference ID | 50305 |
Journal Citation | 36, pp. 237-243 |
Number of Pages | 7 |
Year | 2014 |
Place of Publication | Mackay, Australia |
ISBN | 9781634393782 |
Web Address (URL) of Paper | http://toc.proceedings.com/23743webtoc.pdf |
Conference/Event | 36th Annual Conference of the Australian Society of Sugar Cane Technologists (AASCT 2014) |
Australian Society of Sugar Cane Technologists Conference | |
Event Details | 36th Annual Conference of the Australian Society of Sugar Cane Technologists (AASCT 2014) Event Date 29 Apr 2014 to end of 01 May 2014 Event Location Gold Coast, Australia |
Event Details | Australian Society of Sugar Cane Technologists Conference ASSCT |
Abstract | A new molecular RSD diagnostic platform was developed using polymerase chain reaction (PCR) on pooled leaf sheath biopsies (LSB-PCR). This non-destructive technique samples leaf sheaths from 50 cane stalks, more than tripling the number of stalks routinely surveyed using the current evaporative-binding enzyme-linked immunoassay (EB-EIA) method, and does not require DNA extraction steps. In-field sample collection takes marginally longer, however primary sample processing takes less than a minute, contrasting favourably with the laborious scrubbing, cutting and pumping of cane stalks required by the EB-EIA method. A sample of 31 fields was selected for analysis after returning an EB-EIA absorbance result of >0.05, of which seven were confirmed as having RSD based on phase contrast microscopy (PCM). LSB-PCR detected RSD in six of these seven fields, but also in an additional seven fields presumed to be negative based on EB-EIA/PCM results. The causal agent of RSD, the bacterium Leifsonia xyli subsp. xyli, was confirmed as present by sequencing amplicons from three fields, two for those classed positive and one negative for RSD based on the EB-EIA/PCM method. The LSB-PCR is quicker and more sensitive than the EB-EIA method, and has the potential to provide insights into why RSD persists as a major productivity constraint in the Australian industry. |
Keywords | Leifsonia xyli subsp. xyli, crop resistance, PCR |
ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | NSW Sugar Milling Co-Operative, Australia |
Southern Cross University | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q3w89/novel-diagnostic-method-for-ratoon-stunting-disease-development-and-implications-for-rsd-management
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