Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci

Article

Rust, Michael K., Vetter, Richard, Denholm, Ian, Blagburn, Byron, Williamson, Martin S., Kopp, Steven, Coleman, Glen, Hostetler, Joe, Davis, Wendell, Mencke, Norbert, Rees, Robert, Foit, Sabrina, Bohm, Claudia and Tetzner, Kathrin. 2015. "Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci." Parasitology Research. 114, pp. 7-18. https://doi.org/10.1007/s00436-015-4512-1
Article Title

Susceptibility of adult cat fleas (Siphonaptera: Pulicidae) to insecticides and status of insecticide resistance mutations at the Rdl and knockdown resistance loci

ERA Journal ID15193
Article CategoryArticle
AuthorsRust, Michael K. (Author), Vetter, Richard (Author), Denholm, Ian (Author), Blagburn, Byron (Author), Williamson, Martin S. (Author), Kopp, Steven (Author), Coleman, Glen (Author), Hostetler, Joe (Author), Davis, Wendell (Author), Mencke, Norbert (Author), Rees, Robert (Author), Foit, Sabrina (Author), Bohm, Claudia (Author) and Tetzner, Kathrin (Author)
Journal TitleParasitology Research
Journal Citation114, pp. 7-18
Number of Pages11
Year2015
Place of PublicationGermany
ISSN0932-0113
1432-1955
Digital Object Identifier (DOI)https://doi.org/10.1007/s00436-015-4512-1
Abstract

The susceptibility of 12 field-collected isolates and 4 laboratory strains of cat fleas, Ctenocephalides felis was determined by topical application of some of the insecticides used as on-animal therapies to control them. In the tested field-collected flea isolates the LD50 values for fipronil and imidacloprid ranged from 0.09 to 0.35 ng/flea and 0.02 to 0.19 ng/flea, respectively, and were consistent with baseline figures published previously. The extent of variation in response to four pyrethroid insecticides differed between compounds with the LD50 values for deltamethrin ranging from 2.3 to 28.2 ng/flea, etofenprox ranging from 26.7 to 86.7 ng/flea, permethrin ranging from 17.5 to 85.6 ng/flea, and d-phenothrin ranging from 14.5 to 130 ng/flea. A comparison with earlier data for permethrin and deltamethrin implied a level of pyrethroid resistance in all isolates and strains. LD50 values for tetrachlorvinphos ranged from 20.0 to 420.0 ng/flea. The rdl mutation (conferring target-site resistance to cyclodiene insecticides) was present in most field-collected and laboratory strains, but had no discernible effect on responses to fipronil, which acts on the same receptor protein as cyclodienes. The kdr and skdr mutations conferring target-site resistance to pyrethroids but segregated in opposition to one another, precluding the formation of genotypes homozygous for both mutations.

Keywordsanimals; ctenocephalides; gene expression regulation; genotype; insecticide resistance; insecticides; mutation; siphonaptera; microbiology; bacteriology; mycology
ANZSRC Field of Research 2020310902. Animal cell and molecular biology
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Byline AffiliationsUniversity of California, United States
University of Hertfordshire, United Kingdom
Auburn University, United States
Rothamsted Research, United Kingdom
University of Queensland
Bayer Animal Health, United States
Bayer Animal Health, Germany
Bayer Health Care, Australia
Institution of OriginUniversity of Southern Queensland
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https://research.usq.edu.au/item/q47w6/susceptibility-of-adult-cat-fleas-siphonaptera-pulicidae-to-insecticides-and-status-of-insecticide-resistance-mutations-at-the-rdl-and-knockdown-resistance-loci

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