Genome-Wide Identification and Characterization of Major RNAi Genes Highlighting Their Associated Factors in Cowpea (Vigna unguiculata (L.) Walp.)
Article
Article Title | Genome-Wide Identification and Characterization of Major RNAi |
---|---|
ERA Journal ID | 3417 |
Article Category | Article |
Authors | Hasan, Mohammad Nazmol, Mosharaf, Md Parvez, Uddin, Khandoker Saif, Das, Keya Rani, Sultana, Nasrin, Noorunnahar, Mst., Naim, Darun and Mollah, Md. Nurul Haque |
Journal Title | BioMed Research International |
Journal Citation | 2023 |
Article Number | 832406 |
Number of Pages | 22 |
Year | 2023 |
Publisher | John Wiley & Sons |
Place of Publication | United Kingdom |
ISSN | 1110-7243 |
1110-7251 | |
2314-6133 | |
2314-6141 | |
Digital Object Identifier (DOI) | https://doi.org/10.1155/2023/8832406 |
Web Address (URL) | https://onlinelibrary.wiley.com/doi/10.1155/2023/8832406 |
Abstract | In different regions of the world, cowpea (Vigna unguiculata (L.) Walp.) is an important vegetable and an excellent source of protein. It lessens the malnutrition of the underprivileged in developing nations and has some positive effects on health, such as a reduction in the prevalence of cancer and cardiovascular disease. However, occasionally, certain biotic and abiotic stresses caused a sharp fall in cowpea yield. Major RNA interference (RNAi) genes like Dicer-like (DCL), Argonaute (AGO), and RNA-dependent RNA polymerase (RDR) are essential for the synthesis of their associated factors like domain, small RNAs (sRNAs), transcription factors, micro-RNAs, and cis-acting factors that shield plants from biotic and abiotic stresses. In this study, applying BLASTP search and phylogenetic tree analysis with reference to the Arabidopsis RNAi (AtRNAi) genes, we discovered 28 VuRNAi genes, including 7 VuDCL, 14 VuAGO, and 7 VuRDR genes in cowpea. We looked at the domains, motifs, gene structures, chromosomal locations, subcellular locations, gene ontology (GO) terms, and regulatory factors (transcription factors, micro-RNAs, and cis-acting elements (CAEs)) to characterize the VuRNAi genes and proteins in cowpea in response to stresses. Predicted VuDCL1, VuDCL2(a, b), VuAGO7, VuAGO10, and VuRDR6 genes might have an impact on cowpea growth, development of the vegetative and flowering stages, and antiviral defense. The VuRNAi gene regulatory features miR395 and miR396 might contribute to grain quality improvement, immunity boosting, and pathogen infection resistance under salinity and drought conditions. Predicted CAEs from the VuRNAi genes might play a role in plant growth and development, improving grain quality and production and protecting plants from biotic and abiotic stresses. Therefore, our study provides crucial information about the functional roles of VuRNAi genes and their associated components, which would aid in the development of future cowpeas that are more resilient to biotic and abiotic stress. The manuscript is available as a preprint at this link: doi:10.1101/2023.02.15.528631v1. |
Keywords | abiotic stress; ago10 gene; ago7 gene; Article; basic local alignment search algorithm; biotic stress; cellular distribution; chromosomal localization; computer model; cowpea |
Contains Sensitive Content | Does not contain sensitive content |
ANZSRC Field of Research 2020 | 4299. Other health sciences |
Byline Affiliations | Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh |
School of Business | |
International University of Business Agriculture and Technology, Bangladesh | |
University of Rajshahi, Bangladesh |
https://research.usq.edu.au/item/zq331/genome-wide-identification-and-characterization-of-major-rnai-genes-highlighting-their-associated-factors-in-cowpea-vigna-unguiculata-l-walp
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BioMed Research International - 2023 - Hasan - Genome‐Wide Identification and Characterization of Major RNAi Genes.pdf | ||
License: CC BY 4.0 | ||
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