New smut-specific primers for the ITS barcoding of Ustilaginomycotina
Article
Article Title | New smut-specific primers for the ITS barcoding of Ustilaginomycotina |
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ERA Journal ID | 2503 |
Article Category | Article |
Authors | Kruse, Julia (Author), Choi, Young-Joon (Author) and Thines, Marco (Author) |
Journal Title | Mycological Progress: international journal of fungal sciences |
Journal Citation | 16 (3), pp. 213-221 |
Number of Pages | 9 |
Year | 2017 |
Place of Publication | Germany |
ISSN | 1617-416X |
1861-8952 | |
Digital Object Identifier (DOI) | https://doi.org/10.1007%2Fs11557-016-1265-x |
Web Address (URL) | https://link.springer.com/article/10.1007%2Fs11557-016-1265-x |
Abstract | The smut fungi (Ustilaginomycotina) are a highly diverse group, containing about 115 genera and 1700 species, most of which are biotrophic plant pathogens. As for other fungal groups, the ITS rDNA region is widely used to determine smut fungi at species level due to its high discriminatory power and for phylogenetic reconstructions within genera. So far, two primer sets, ITS1/ITS4 and M-ITS1/ITS4, were generally used to amplify smut fungi, but these often co-amplify host plants or contaminant fungi and do not yield satisfactory amplification for a variety of smut fungi. In the present study, based on a selection of genera that include more than 90% of the species of smut fungi (more than half of the genera of smut fungi), three new primers, smITS-F, smITS-R1 and smITSR2, situated in the SSU or LSU region, were designed to avoid the amplification of host plants and to extend the coverage of PCR amplification for as many smut genera as possible. |
Keywords | Barcoding locus; Basidiomycota; Diversity; DNA-extraction methods; Smut-specific primers |
ANZSRC Field of Research 2020 | 310705. Mycology |
310299. Bioinformatics and computational biology not elsewhere classified | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Senckenberg Biodiversity and Climate Research Centre, Germany |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q52zv/new-smut-specific-primers-for-the-its-barcoding-of-ustilaginomycotina
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