Proteomic profiling of gamma-carboxylated proteins in horse plasma upon vitamin K supplementation
Poster
Paper/Presentation Title | Proteomic profiling of gamma-carboxylated proteins in horse plasma upon vitamin K supplementation |
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Presentation Type | Poster |
Authors | Skinner, Jazmine E. (Author), Sadowski, Pawel (Author), Satake, Nana (Author), Regtop, Hubert L. (Author), Biffin, Ray (Author), Cawdell-Smith, Allison J. (Author) and Bryden, Wayne L. (Author) |
Journal or Proceedings Title | Proceedings of the 6th International Vitamin Conference |
Number of Pages | 1 |
Year | 2021 |
Place of Publication | Copenhagen, Denmark |
Web Address (URL) of Paper | https://vitaminconference.com/ |
Conference/Event | 6th International Vitamin Conference (IVC 2021) |
Event Details | 6th International Vitamin Conference (IVC 2021) Event Location Copenhagen, Denmark |
Abstract | Introduction: Vitamin K is a cofactor for γ-carboxyglutamase to convert glutamate (Glu) residues to γ-carboxyglutamic acid (Gla) residues in proteins. Few studies have investigated the relationship between vitamin K intake, and its effect on circulating vitamin K dependent proteins (VKDPs) in horses. It is suspected that vitamin K supplementation of mares may change the VKDP plasma proteome of foals. To date, no studies have investigated the applicability of a proteomics-based approach to evaluate this. The aim of this study was to facilitate the detection of carboxylated VKDPs in plasma by applying a novel method; Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE). Method: 12 mares were allocated to a treatment; K1 and K2 (15mg) or control; blank paste. Treatments were administered orally, from parturition until 3 months post-parturition. Blood samples were collected from foals before suckling, at 12 h, 24 h and weekly up until 3 months. Plasma samples were analysed for vitamin K1 concentration by HPLC, and proteomic analysis by LC-MS/MS. Results: This study applied the CACHE protocol to plasma samples collected from 14-day old foals, that showed a statistically significant difference in vitamin K1 concentration (P<0.05). While it was anticipated that supplementation would facilitate the detection of VKDPs by CACHE, no VKDPs of interest were identified in this preliminary study. Carboxylation however, was detected. Conclusion: To the author’s knowledge, this is the first study to investigate a novel proteomics-based method to |
Keywords | Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment |
ANZSRC Field of Research 2020 | 300301. Animal growth and development |
300303. Animal nutrition | |
310109. Proteomics and intermolecular interactions (excl. medical proteomics) | |
Public Notes | Abstract #67. |
Byline Affiliations | School of Sciences |
Queensland University of Technology | |
University of Queensland | |
Agricure, Australia | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q6vww/proteomic-profiling-of-gamma-carboxylated-proteins-in-horse-plasma-upon-vitamin-k-supplementation
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