Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals

Presentation


Skinner, Jazmine E., Sadowski, Pawel, Satake, Nana, Regtop, Hubert L., Biffin, Ray, Cawdell-Smith, Allison J. and Bryden, Wayne L.. 2021. "Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals." 2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021). Gold Coast, Australia 09 - 11 Jun 2021 Australia.
Paper/Presentation Title

Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals

Presentation TypePresentation
AuthorsSkinner, Jazmine E. (Author), Sadowski, Pawel (Author), Satake, Nana (Author), Regtop, Hubert L. (Author), Biffin, Ray (Author), Cawdell-Smith, Allison J. (Author) and Bryden, Wayne L. (Author)
Journal or Proceedings Title2021 Recent Advancement in Animal Nutrition Australia Conference (RAAN 2021): Book of Abstracts
Number of Pages1
Year2021
Place of PublicationAustralia
Web Address (URL) of Paperhttps://www.raan.com.au/
Conference/Event2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021)
Event Details
2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021)
Event Date
09 to end of 11 Jun 2021
Event Location
Gold Coast, Australia
Abstract

Introduction
Vitamin K is a cofactor for γ-carboxyglutamase to convert glutamate (Glu) residues to γ-carboxyglutamic acid (Gla) residues in proteins. Few studies have investigated the relationship between vitamin K intake, and its effect on circulating vitamin K dependent proteins (VKDPs) in horses. It is suspected that vitamin K supplementation of mares may change the VKDP plasma proteome of foals. To date, no studies that have investigated the applicability of a proteomics-based approach to evaluate this. The aim of this study was to facilitate the detection of carboxylated VKDPs in plasma by applying the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method.

Method
12 mares were allocated to a treatment; K1 and K2 (15mg) or control; blank paste. Treatments were administered orally, from parturition until 3 months post-parturition. Blood samples were collected from foals before suckling, at 12 h, 24 h and weekly up until 3 months. Plasma samples were analysed for vitamin K1 concentration by HPLC, and proteomic analysis by LC-MS/MS.

Results
This study applied the CACHE protocol to plasma samples collected from 14-day old foals, that showed a statistically significant difference in vitamin K1 concentration (P<0.05). While it was anticipated that supplementation would facilitate the detection of carboxylated VKDPs by CACHE, no key VKDPs of interest were identified.

Conclusion
To the author’s knowledge, this is the first study to investigate a novel proteomics-based method to decipher VKDPs in equine plasma. This novel approach will facilitate future studies to investigate the function of VKDPs in the horse, and other species.

Keywordsvitamin k, horses
ANZSRC Field of Research 2020300303. Animal nutrition
310109. Proteomics and intermolecular interactions (excl. medical proteomics)
Byline AffiliationsSchool of Sciences
Queensland University of Technology
University of Queensland
Agricure, Australia
Institution of OriginUniversity of Southern Queensland
Permalink -

https://research.usq.edu.au/item/q6vx0/proteomic-evaluation-of-the-vitamin-k-status-of-horses-application-of-the-carboxylated-analysis-by-ceramic-hydroxyapatite-enrichment-cache-method-to-the-plasma-of-vitamin-k-supplemented-foals

  • 122
    total views
  • 3
    total downloads
  • 1
    views this month
  • 0
    downloads this month

Export as

Related outputs

Scaffolding experiential learning across an agricultural technology program
Baillie, Justine, Scobie, Michael, Long, Derek, Skinner, Jazmine and Humpal, Jacob. 2024. "Scaffolding experiential learning across an agricultural technology program." 3rd Symposium for Agricultural Educators (AgEd 24). Rockhampton, Australia 06 - 07 Feb 2024 Australia.
Effect of phenylbutazone on insulin secretion in horses with insulin dysregulation
Kemp, Kate L., Skinner, Jazmine E. and Bertin, François-René. 2024. "Effect of phenylbutazone on insulin secretion in horses with insulin dysregulation." Journal of Veterinary Internal Medicine. 38 (2), pp. 1177-1184. https://doi.org/10.1111/jvim.17013
Vitamin K: history, metabolism, and nutrition in the horse
Skinner, Jazmine E., Cawdell-Smith, A. Judy, Biffin, J. Ray, Regtop, Hubert L. and Bryden, Wayne L.. 2023. "Vitamin K: history, metabolism, and nutrition in the horse ." Animal Production Science. https://doi.org/10.1071/AN22319
The Use of Percutaneous Thermal Sensing Microchips to Measure Body Temperature in Horses during and after Exercise Using Three Different Cool-Down Methods
Kang, Hyungsuk, Zsoldos, Rebeka R., Skinner, Jazmine E., Gaughan, John B., Mellor, Vincent A. and Sole-Guitart, Albert. 2022. "The Use of Percutaneous Thermal Sensing Microchips to Measure Body Temperature in Horses during and after Exercise Using Three Different Cool-Down Methods." Animals. 12 (10), pp. 1-20. https://doi.org/10.3390/ani12101267
Pharmacokinetic evaluation of a novel transdermal ketoprofen formulation in healthy dogs
Ravuri, Halley Gora, Satake, Nana, Balmanno, Alexandra, Skinner, Jazmine, Kempster, Samantha and Mills, Paul C.. 2022. "Pharmacokinetic evaluation of a novel transdermal ketoprofen formulation in healthy dogs." Pharmaceutics. 14 (3), pp. 1-11. https://doi.org/10.3390/pharmaceutics14030646
Comparison of post-exercise cooling methods in horses
Kang, H., Zsoldos, R. R., Skinner, J. E., Gaughan, J. B. and Guitart, A. S.. 2021. "Comparison of post-exercise cooling methods in horses." 2021 Equine Science Society Virtual Symposium. Champaign, United States 01 - 04 Jun 2021 United States. https://doi.org/10.1016/j.jevs.2021.103485
Vitamin K absorption in the horse: intestinal uptake of different vitamers
Skinner, J. E., Cawdell-Smith, A. J., Biffin, J. R., Talbot, A. M., Regtop, H. L. and Bryden, W. L.. 2014. "Vitamin K absorption in the horse: intestinal uptake of different vitamers." 2014 Australasian Equine Science Symposium. Gold Coast, Australia 18 - 20 Jul 2014 Brisbane, Australia.
Vitamin K absorption in the horse: does absorption occur from the hindgut
Regtop, H. L., Talbot, A. M., Biffin, J. R., Skinner, J. E., Cawdell-Smith, A. J. and Bryden, W. L.. 2014. "Vitamin K absorption in the horse: does absorption occur from the hindgut." 2014 Australasian Equine Science Symposium. Gold Coast, Australia 18 - 20 Jul 2014 Brisbane, Australia.
Proteomic profiling of gamma-carboxylated proteins in horse plasma upon vitamin K supplementation
Skinner, Jazmine E., Sadowski, Pawel, Satake, Nana, Regtop, Hubert L., Biffin, Ray, Cawdell-Smith, Allison J. and Bryden, Wayne L.. 2021. "Proteomic profiling of gamma-carboxylated proteins in horse plasma upon vitamin K supplementation." 6th International Vitamin Conference (IVC 2021). Copenhagen, Denmark Copenhagen, Denmark.
Vitamin K and equine osteocalcin: an enigma explored
Skinner, Jazmine Elizabeth. 2020. Vitamin K and equine osteocalcin: an enigma explored. PhD Thesis Doctor of Philosophy. University of Queensland. https://doi.org/10.14264/b8fc040
Equine production systems and the changing role of horses in society
Skinner, Jazmine E., Hilly, Lynette J., Li, Xiuhua, Cawdell-Smith, A .Judy and Bryden, Wayne L.. 2019. "Equine production systems and the changing role of horses in society." Squires, Victor Roy and Bryden, Wayne L. (ed.) Livestock: production, management strategies and challenges. New York, United States. Nova Science Publishers. pp. 389-433
Intestinal absorption of different vitamin K compounds in the horse
Skinner, J. E., Cawdell-Smith, A. J., Biffin, J. R., Talbot, A. M., Regtop, H. L. and Bryden, W. L.. 2015. "Intestinal absorption of different vitamin K compounds in the horse." 2015 Equine Science Society Symposium. Florida, United States of America 26 - 29 May 2015 United States. https://doi.org/10.1016/j.jevs.2015.03.018
Extent of vitamin K absorption from the equine hindgut
Skinner, J. E., Cawdell-Smith, A. J., Regtop, H. L., Talbot, A. M., Biffin, J. R. and Bryden, W. L.. 2015. "Extent of vitamin K absorption from the equine hindgut." 2015 Equine Science Society Symposium. Florida, United States of America 26 - 29 May 2015 United States. https://doi.org/10.1016/j.jevs.2015.03.069