Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals

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Skinner, Jazmine E., Sadowski, Pawel, Satake, Nana, Regtop, Hubert L., Biffin, Ray, Cawdell-Smith, Allison J. and Bryden, Wayne L.. 2021. "Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals." 2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021). Gold Coast, Australia 09 - 11 Jun 2021 Australia.
Paper/Presentation Title

Proteomic evaluation of the vitamin K status of horses: application of the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method to the plasma of vitamin K supplemented foals

Presentation TypePresentation
AuthorsSkinner, Jazmine E. (Author), Sadowski, Pawel (Author), Satake, Nana (Author), Regtop, Hubert L. (Author), Biffin, Ray (Author), Cawdell-Smith, Allison J. (Author) and Bryden, Wayne L. (Author)
Journal or Proceedings Title2021 Recent Advancement in Animal Nutrition Australia Conference (RAAN 2021): Book of Abstracts
Number of Pages1
Year2021
Place of PublicationAustralia
Web Address (URL) of Paperhttps://www.raan.com.au/
Conference/Event2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021)
Event Details
2021 Recent Advancement in Animal Nutrition - Australia Conference (RAAN 2021)
Event Date
09 to end of 11 Jun 2021
Event Location
Gold Coast, Australia
Abstract

Introduction
Vitamin K is a cofactor for γ-carboxyglutamase to convert glutamate (Glu) residues to γ-carboxyglutamic acid (Gla) residues in proteins. Few studies have investigated the relationship between vitamin K intake, and its effect on circulating vitamin K dependent proteins (VKDPs) in horses. It is suspected that vitamin K supplementation of mares may change the VKDP plasma proteome of foals. To date, no studies that have investigated the applicability of a proteomics-based approach to evaluate this. The aim of this study was to facilitate the detection of carboxylated VKDPs in plasma by applying the Carboxylated Analysis by Ceramic Hydroxyapatite Enrichment (CACHE) method.

Method
12 mares were allocated to a treatment; K1 and K2 (15mg) or control; blank paste. Treatments were administered orally, from parturition until 3 months post-parturition. Blood samples were collected from foals before suckling, at 12 h, 24 h and weekly up until 3 months. Plasma samples were analysed for vitamin K1 concentration by HPLC, and proteomic analysis by LC-MS/MS.

Results
This study applied the CACHE protocol to plasma samples collected from 14-day old foals, that showed a statistically significant difference in vitamin K1 concentration (P<0.05). While it was anticipated that supplementation would facilitate the detection of carboxylated VKDPs by CACHE, no key VKDPs of interest were identified.

Conclusion
To the author’s knowledge, this is the first study to investigate a novel proteomics-based method to decipher VKDPs in equine plasma. This novel approach will facilitate future studies to investigate the function of VKDPs in the horse, and other species.

Keywordsvitamin k, horses
ANZSRC Field of Research 2020300303. Animal nutrition
310109. Proteomics and intermolecular interactions (excl. medical proteomics)
Byline AffiliationsSchool of Sciences
Queensland University of Technology
University of Queensland
Agricure, Australia
Institution of OriginUniversity of Southern Queensland
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https://research.usq.edu.au/item/q6vx0/proteomic-evaluation-of-the-vitamin-k-status-of-horses-application-of-the-carboxylated-analysis-by-ceramic-hydroxyapatite-enrichment-cache-method-to-the-plasma-of-vitamin-k-supplemented-foals

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