Respiratory eukaryotic virome expansion and bacteriophage deficiency characterize childhood asthma
Article
Article Title | Respiratory eukaryotic virome expansion and bacteriophage deficiency characterize childhood asthma |
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ERA Journal ID | 201487 |
Article Category | Article |
Authors | Megremis, Spyridon, Constantinides, Bede, Xepapadaki, Paraskevi, Yap, Chuan Fu, Sotiropoulos, Alexandros G., Bachert, Claus, Finotto, Susetta, Jartti, Tuomas, Tapinos, Avraam, Vuorinen, Tytti, Andreakos, Evangelos, Robertson, David L. and Papadopoulos, Nikolaos G. |
Journal Title | Scientific Reports |
Journal Citation | 13 (1) |
Number of Pages | 14 |
Year | 2023 |
Publisher | Nature Publishing Group |
Place of Publication | United Kingdom |
ISSN | 2045-2322 |
Digital Object Identifier (DOI) | https://doi.org/10.1038/s41598-023-34730-7 |
Web Address (URL) | https://www.nature.com/articles/s41598-023-34730-7 |
Abstract | Asthma development and exacerbation is linked to respiratory virus infections. There is limited information regarding the presence of viruses during non-exacerbation/infection periods. We investigated the nasopharyngeal/nasal virome during a period of asymptomatic state, in a subset of 21 healthy and 35 asthmatic preschool children from the Predicta cohort. Using metagenomics, we described the virome ecology and the cross-species interactions within the microbiome. The virome was dominated by eukaryotic viruses, while prokaryotic viruses (bacteriophages) were independently observed with low abundance. Rhinovirus B species consistently dominated the virome in asthma. Anelloviridae were the most abundant and rich family in both health and asthma. However, their richness and alpha diversity were increased in asthma, along with the co-occurrence of different Anellovirus genera. Bacteriophages were richer and more diverse in healthy individuals. Unsupervised clustering identified three virome profiles that were correlated to asthma severity and control and were independent of treatment, suggesting a link between the respiratory virome and asthma. Finally, we observed different cross-species ecological associations in the healthy versus the asthmatic virus-bacterial interactome, and an expanded interactome of eukaryotic viruses in asthma. Upper respiratory virome “dysbiosis” appears to be a novel feature of pre-school asthma during asymptomatic/non-infectious states and merits further investigation. |
Keywords | Anelloviridae; Asthma; Asymptomatic Diseases; Bacteriophages; Child; Eukaryota; Eukaryotic Cells; Humans; Virome |
ANZSRC Field of Research 2020 | 320705. Medical virology |
329999. Other biomedical and clinical sciences not elsewhere classified | |
310599. Genetics not elsewhere classified | |
Byline Affiliations | University of Manchester, United Kingdom |
University of Leicester, United Kingdom | |
University of Oxford, United Kingdom | |
National and Kapodistrian University of Athens, Greece | |
University of Zurich, Switzerland | |
Centre for Crop Health | |
University of Münster, Germany | |
Friedrich Alexander University Erlangen-Nurnberg, Germany | |
University of Turku, Finland | |
University of Oulu, Finland | |
Academy of Athens, Greece | |
University of Glasgow, United Kingdom |
https://research.usq.edu.au/item/z34xy/respiratory-eukaryotic-virome-expansion-and-bacteriophage-deficiency-characterize-childhood-asthma
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