Respiratory eukaryotic virome expansion and bacteriophage deficiency characterize childhood asthma

Article


Megremis, Spyridon, Constantinides, Bede, Xepapadaki, Paraskevi, Yap, Chuan Fu, Sotiropoulos, Alexandros G., Bachert, Claus, Finotto, Susetta, Jartti, Tuomas, Tapinos, Avraam, Vuorinen, Tytti, Andreakos, Evangelos, Robertson, David L. and Papadopoulos, Nikolaos G.. 2023. "Respiratory eukaryotic virome expansion and bacteriophage deficiency characterize childhood asthma." Scientific Reports. 13 (1). https://doi.org/10.1038/s41598-023-34730-7
Article Title

Respiratory eukaryotic virome expansion and bacteriophage deficiency characterize childhood asthma

ERA Journal ID201487
Article CategoryArticle
AuthorsMegremis, Spyridon, Constantinides, Bede, Xepapadaki, Paraskevi, Yap, Chuan Fu, Sotiropoulos, Alexandros G., Bachert, Claus, Finotto, Susetta, Jartti, Tuomas, Tapinos, Avraam, Vuorinen, Tytti, Andreakos, Evangelos, Robertson, David L. and Papadopoulos, Nikolaos G.
Journal TitleScientific Reports
Journal Citation13 (1)
Number of Pages14
Year2023
PublisherNature Publishing Group
Place of PublicationUnited Kingdom
ISSN2045-2322
Digital Object Identifier (DOI)https://doi.org/10.1038/s41598-023-34730-7
Web Address (URL)https://www.nature.com/articles/s41598-023-34730-7
Abstract

Asthma development and exacerbation is linked to respiratory virus infections. There is limited information regarding the presence of viruses during non-exacerbation/infection periods. We investigated the nasopharyngeal/nasal virome during a period of asymptomatic state, in a subset of 21 healthy and 35 asthmatic preschool children from the Predicta cohort. Using metagenomics, we described the virome ecology and the cross-species interactions within the microbiome. The virome was dominated by eukaryotic viruses, while prokaryotic viruses (bacteriophages) were independently observed with low abundance. Rhinovirus B species consistently dominated the virome in asthma. Anelloviridae were the most abundant and rich family in both health and asthma. However, their richness and alpha diversity were increased in asthma, along with the co-occurrence of different Anellovirus genera. Bacteriophages were richer and more diverse in healthy individuals. Unsupervised clustering identified three virome profiles that were correlated to asthma severity and control and were independent of treatment, suggesting a link between the respiratory virome and asthma. Finally, we observed different cross-species ecological associations in the healthy versus the asthmatic virus-bacterial interactome, and an expanded interactome of eukaryotic viruses in asthma. Upper respiratory virome “dysbiosis” appears to be a novel feature of pre-school asthma during asymptomatic/non-infectious states and merits further investigation.

KeywordsAnelloviridae; Asthma; Asymptomatic Diseases; Bacteriophages; Child; Eukaryota; Eukaryotic Cells; Humans; Virome
ANZSRC Field of Research 2020320705. Medical virology
329999. Other biomedical and clinical sciences not elsewhere classified
310599. Genetics not elsewhere classified
Byline AffiliationsUniversity of Manchester, United Kingdom
University of Leicester, United Kingdom
University of Oxford, United Kingdom
National and Kapodistrian University of Athens, Greece
University of Zurich, Switzerland
Centre for Crop Health
University of Münster, Germany
Friedrich Alexander University Erlangen-Nurnberg, Germany
University of Turku, Finland
University of Oulu, Finland
Academy of Athens, Greece
University of Glasgow, United Kingdom
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