Detection and identification of Bogia coconut syndrome phytoplasma from seed-associated tissues and seedlings of coconut (Cocos nucifera) and betel nut (Areca catechu)
Article
Lu, Hengyu, Wilson, Bree, Zhang, Hanfang, Woruba, Sharon B., Feng, Bowen, Johnson, Anne C., Komolong, Birte, Kuniata, Lastus, Yang, Guang and Gurr, Geof M.. 2024. "Detection and identification of Bogia coconut syndrome phytoplasma from seed-associated tissues and seedlings of coconut (Cocos nucifera) and betel nut (Areca catechu)." Scientific Reports. 14 (1). https://doi.org/10.1038/s41598-024-61916-4
| Article Title | Detection and identification of Bogia coconut syndrome phytoplasma from seed-associated tissues and seedlings of coconut (Cocos nucifera) and betel nut (Areca catechu) |
|---|---|
| ERA Journal ID | 201487 |
| Article Category | Article |
| Authors | Lu, Hengyu, Wilson, Bree, Zhang, Hanfang, Woruba, Sharon B., Feng, Bowen, Johnson, Anne C., Komolong, Birte, Kuniata, Lastus, Yang, Guang and Gurr, Geof M. |
| Journal Title | Scientific Reports |
| Journal Citation | 14 (1) |
| Number of Pages | 9 |
| Year | 2024 |
| Publisher | Nature Publishing Group |
| Place of Publication | United Kingdom |
| ISSN | 2045-2322 |
| Digital Object Identifier (DOI) | https://doi.org/10.1038/s41598-024-61916-4 |
| Web Address (URL) | https://www.nature.com/articles/s41598-024-61916-4 |
| Abstract | Evidence for seed transmission of phytoplasmas has grown in several pathosystems including coconut (Cocos nucifera). Bogia coconut syndrome (BCS) is a disease associated with the lethal yellowing syndrome associated with the presence of ‘Candidatus Phytoplasma noviguineense’ that affects coconut, betel nut (Areca catechu) and bananas (Musa spp.) in Papua New Guinea. Coconut and betel nut drupes were sampled from BCS-infected areas in Papua New Guinea, dissected, the extracted nucleic acid was used in polymerase chain reaction (PCR), and loop mediated isothermal amplification (LAMP) used to check for presence of phytoplasma DNA. In a second study, drupes of both plant species were collected from multiple field sites and grown in insect-proof cages. Leaf samples taken at 6 months were also tested with PCR and LAMP. The studies of dissected coconut drupes detected phytoplasma DNA in several tissues including the embryo. Drupes from betel nut tested negative. Among the seedlings, evidence of possible seed transmission was found in both plant species. The results demonstrate the presence of ‘Ca. P. noviguineense’ in coconut drupes and seedlings, and in seedlings of betel nut; factors that need to be considered in ongoing management and containment efforts. |
| Keywords | 16S rRNA; Seed borne; Seed transmission; Lethal yellowing disease |
| Contains Sensitive Content | Does not contain sensitive content |
| ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
| Byline Affiliations | Fujian Agriculture and Forestry University, China |
| Ministry of Agriculture Fuzhou, China | |
| Fujian Province University, China | |
| Centre for Crop Health | |
| Institute for Life Sciences and the Environment | |
| Statutory, Regulatory Institution for the Coconut Industry, Papua New Guinea | |
| Charles Sturt University | |
| National Agricultural Research Institute, Papua New Guinea | |
| New Britain Palm Oil, Ramu Agri Industries, Papua New Guinea |
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https://research.usq.edu.au/item/z852z/detection-and-identification-of-bogia-coconut-syndrome-phytoplasma-from-seed-associated-tissues-and-seedlings-of-coconut-cocos-nucifera-and-betel-nut-areca-catechu
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