Determining putative vectors of the Bogia Coconut Syndrome Phytoplasma using loop-mediated isothermal amplification of single insect-feeding media
Article
Article Title | Determining putative vectors of the Bogia Coconut Syndrome Phytoplasma using loop-mediated isothermal amplification of single insect-feeding media |
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ERA Journal ID | 201487 |
Article Category | Article |
Authors | Lu, Hengyu (Author), Wilson, Bree A. L. (Author), Ash, Gavin J. (Author), Woruba, Sharon B. (Author), Fletcher, Murray J. (Author), You, Minsheng (Author), Yang, Guang (Author) and Gurr, Geoff M. (Author) |
Journal Title | Scientific Reports |
Journal Citation | 6, pp. 1-10 |
Article Number | 35801 |
Number of Pages | 10 |
Year | 2016 |
Publisher | Nature Publishing Group |
Place of Publication | United Kingdom |
ISSN | 2045-2322 |
Digital Object Identifier (DOI) | https://doi.org/10.1038/srep35801 |
Web Address (URL) | http://www.nature.com/articles/srep35801 |
Abstract | Phytoplasmas are insect vectored mollicutes responsible for disease in many economically important crops. Determining which insect species are vectors of a given phytoplasma is important for managing disease but is methodologically challenging because disease-free plants need to be exposed to large numbers of insects, often over many months. A relatively new method to detect likely transmission involves molecular testing for phytoplasma DNA in sucrose solution that insects have fed upon. In this study we combined this feeding medium method with a loop-mediated isothermal amplification (LAMP) assay to study 627 insect specimens of 11 Hemiptera taxa sampled from sites in Papua New Guinea affected by Bogia coconut syndrome (BCS). The LAMP assay detected phytoplasma DNA from the feeding solution and head tissue of insects from six taxa belonging to four families: Derbidae, Lophopidae, Flatidae and Ricaniidae. Two other taxa yielded positives only from the heads and the remainder tested negative. These results demonstrate the utility of combining single-insect feeding medium tests with LAMP assays to identify putative vectors that can be the subject of transmission tests and to better understand phytoplasma pathosystems. |
Keywords | Bogia Coconut Syndrome (BCS); phytoplasmas; palms; coconut palms; disease management; loop-mediated isothermal amplification (LAMP) |
ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
Byline Affiliations | Fujian Agriculture and Forestry University, China |
Centre for Crop Health | |
Cocoa and Coconut Research Institute, Papua New Guinea | |
Charles Sturt University | |
Institution of Origin | University of Southern Queensland |
Funding source | Grant ID Australian Centre for International Agricultural Research (grant PC/2012/087) |
https://research.usq.edu.au/item/q3q4q/determining-putative-vectors-of-the-bogia-coconut-syndrome-phytoplasma-using-loop-mediated-isothermal-amplification-of-single-insect-feeding-media
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