Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae
Article
Article Title | Identification of virulence associated loci in the emerging broad host range plant pathogen Pseudomonas fuscovaginae |
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ERA Journal ID | 2523 |
Article Category | Article |
Authors | Patel, Hitendra Kumar (Author), Matiuzzo, Maura (Author), Bertani, Iris (Author), Bigirimana, Vincent De Paul (Author), Ash, Gavin J. (Author), Hofte, Monica (Author) and Venturi, Vittoria (Author) |
Journal Title | BMC Microbiology |
Journal Citation | 14 (274), pp. 1-13 |
Number of Pages | 13 |
Year | 2014 |
Place of Publication | United Kingdom |
ISSN | 1471-2180 |
Digital Object Identifier (DOI) | https://doi.org/10.1186/s12866-014-0274-7 |
Web Address (URL) | http://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-014-0274-7 |
Abstract | BACKGROUND: Pseudomonas fuscovaginae (Pfv) is an emerging plant pathogen of rice and also of other gramineae plants. It causes sheath brown rot disease in rice with symptoms that are characterized by brown lesions on the flag leaf sheath, grain discoloration and sterility. It was first isolated as a high altitude pathogen in Japan and has since been reported in several countries throughout the world. Pfv is a broad host range pathogen and very little is known about its virulence mechanisms. RESULTS: An in planta screen of 1000 random independent Tn5 genomic mutants resulted in the isolation of nine mutants which showed altered virulence. Some of these isolates are mutated for functions which are known to be virulence associated factors in other phytopathogenic bacteria (eg. pil gene, phytotoxins and T6SS) and others might represent novel virulence loci. CONCLUSIONS: Being an emerging pathogen worldwide, the broad host range pathogen Pfv has not yet been studied for its virulence functions. The roles of the nine loci identified in the in planta screen are discussed in relation to pathogenicity of Pfv. In summary, this article reports a first study on the virulence of this pathogen involving in planta screening studies and suggests the presence of several virulence features with known and novel functions in the Pseudomonas group of bacteria. |
Keywords | DNA Transposable Elements; Gene Knockout Techniques; Japan; Mutagenesis, Insertional; Plant Diseases; Poaceae; Pseudomonas; Virulence |
ANZSRC Field of Research 2020 | 310899. Plant biology not elsewhere classified |
Byline Affiliations | International Centre for Genetic Engineering and Biotechnology, Italy |
Charles Sturt University | |
Ghent University, Belgium | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q399q/identification-of-virulence-associated-loci-in-the-emerging-broad-host-range-plant-pathogen-pseudomonas-fuscovaginae
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