Development of strain specific molecular markers for the Sclerotinia minor bioherbicide strain IMI 344141
Article
Article Title | Development of strain specific molecular markers for the Sclerotinia minor bioherbicide strain IMI 344141 |
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ERA Journal ID | 3334 |
Article Category | Article |
Authors | Pan, L., Ash, G. J., Ahn, B. and Watson, A. K. |
Journal Title | Biocontrol Science and Technology |
Journal Citation | 20 (9), pp. 939-959 |
Number of Pages | 21 |
Year | 2010 |
Publisher | Taylor & Francis |
Place of Publication | United Kingdom |
ISSN | 0958-3157 |
1360-0478 | |
Digital Object Identifier (DOI) | https://doi.org/10.1080/09583157.2010.491895 |
Web Address (URL) | https://www.tandfonline.com/doi/full/10.1080/09583157.2010.491895 |
Abstract | The plant pathogenic fungus, Sclerotinia minor IMI 344141, has been developed as a bioherbicide for broadleaf weed control in turfgrass and a means to differentiate this biocontrol agent from like organisms is required. A strain specific molecular marker was developed to detect and monitor the Sclerotinia minor IMI 344141 bioherbicide strain. The method was based on polymerase chain reaction (PCR) amplification of two sequence-characterized amplified regions (SCAR) primer pairs for a first round PCR, and another two sets of nested primers was used for a second round PCR if higher sensitivity was needed. Sclerotinia minor IMI 344141 was successfully traced from both pure cultures and environmental samples originating from bioherbicide-released field trials. DNA of the S. minor bioherbicide isolate IMI 344141 was detected in the soil 2 months after application, but was not detected in the 3- and 9-month samples after application. When applied as a bioherbicide, S. minor (IMI 344141) did not persist into the following spring season in turf environments. This molecular detection method provides a mechanism to distinguish this isolate from related organisms and a tool to monitor behavior of the biocontrol agent S. minor IMI 344141 in nature, particularly in soil. |
Keywords | biocontrol; bioherbicide; detection; mycoherbicide; PCR; RAPD; SCAR; Sclerotinia minor; turfgrass |
Public Notes | There are no files associated with this item. |
Byline Affiliations | Charles Sturt University |
https://research.usq.edu.au/item/v88v6/development-of-strain-specific-molecular-markers-for-the-sclerotinia-minor-bioherbicide-strain-imi-344141
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