Iterative germination and innovative techniques for the production and inoculation of secondary conidia of sorghum ergot (Claviceps africana)

Article


Tonapi, V. A., Ryley, M., Galea, V., Bhuiyan, S. and Wearing, A.. 2009. "Iterative germination and innovative techniques for the production and inoculation of secondary conidia of sorghum ergot (Claviceps africana) ." Archives of Phytopathology and Plant Protection. 42 (3), pp. 248-255. https://doi.org/10.1080/03235400601036612
Article Title

Iterative germination and innovative techniques for the production and inoculation of secondary conidia of sorghum ergot (Claviceps africana)

ERA Journal ID44687
Article CategoryArticle
AuthorsTonapi, V. A. (Author), Ryley, M. (Author), Galea, V. (Author), Bhuiyan, S. (Author) and Wearing, A. (Author)
Journal TitleArchives of Phytopathology and Plant Protection
Journal Citation42 (3), pp. 248-255
Number of Pages8
Year2009
Place of PublicationAbingdon, OX. United Kingdom
ISSN0323-5408
Digital Object Identifier (DOI)https://doi.org/10.1080/03235400601036612
Web Address (URL)http://www.tandfonline.com/doi/abs/10.1080/03235400601036612
Abstract

Five methods of harvesting pure secondary conidia were attempted, but only one was successful. The only successful method involved moving air across the surface of water agar plates or moist soil on which secondary sporulation was occurring at 0.2 – 3 m/s. Among the inoculation techniques, brushing the secondary conidia on to the wet stigma gave maximum ergot infection (80%) followed by brushing the
secondary conidia on to the dry stigma (77%). Conidia of Claviceps africana produced up to seven generations of sibling conidia. The length and width of secondary conidia showed a reduction in size from 10.92 – 10.13 mm and 5.49 – 5.13 mm, respectively, across generations. However no definite trend in decline in size of secondary conidia was evident. The optimum temperature range for germination of
secondary conidia of Australian isolates of C. africana is between 14.6 and 208C.

Keywordsiterative; germination; ergot; sorghum (Sorghum bicolor (L.) Moench); secondary conidia; simple techniques
ANZSRC Field of Research 2020310701. Bacteriology
300406. Crop and pasture improvement (incl. selection and breeding)
300409. Crop and pasture protection (incl. pests, diseases and weeds)
Public Notes

© 2009 Taylor & Francis. Published version deposited in accordance with the copyright policy of the publisher.

Byline AffiliationsCentre for Rabi Sorghum, India
Department of Primary Industries and Fisheries, Queensland
University of Queensland
Institution of OriginUniversity of Southern Queensland
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