Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repens L.) Outcrossed with High Anthocyanin Lines

Article


Roldan, Marissa B., Cousins, Greig, Fraser, Karl, Hancock, Kerry R., Collette, Vern, Demmer, Jerome, Woodfield, Derek R., Caradus, John R., Jones, Chris and Voisey, Christine R.. 2020. "Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repens L.) Outcrossed with High Anthocyanin Lines." Journal of Agricultural and Food Chemistry. 68 (10), pp. 2927-2939. https://doi.org/10.1021/acs.jafc.9b01185
Article Title

Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repens L.) Outcrossed with High Anthocyanin Lines

ERA Journal ID1349
Article CategoryArticle
AuthorsRoldan, Marissa B., Cousins, Greig, Fraser, Karl, Hancock, Kerry R., Collette, Vern, Demmer, Jerome, Woodfield, Derek R., Caradus, John R., Jones, Chris and Voisey, Christine R.
Journal TitleJournal of Agricultural and Food Chemistry
Journal Citation68 (10), pp. 2927-2939
Year2020
PublisherAmerican Chemical Society
Place of PublicationUnited States
ISSN0021-8561
1520-5118
Digital Object Identifier (DOI)https://doi.org/10.1021/acs.jafc.9b01185
Web Address (URL)https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.9b01185
Abstract

Condensed tannins (CT) are highly desirable in forage as they sequester dietary protein and reduce bloat and methane emissions in ruminants. However, the widely used forage legume white clover (Trifolium repens) only produces CTs in flowers and trichomes and at levels too low to achieve therapeutic effects. Genetic transformation with transcription factor Ta-MYB14-1 from Trifolium arvense was effective in inducing CTs to 0.6% of leaf dry matter. CT synthesis has been elevated further by crossing the primary white clover transgenic line with wild type genotypes producing the related phenylpropanoids, anthocyanins. CT levels in leaves were highest under the anthocyanin leaf marks associated with the "red midrib" trait; however, there was no evidence for CT accumulation in leaf sections with the "red V" anthocyanin marking. Ta-MYB14-1 was stably inherited in two generations of crosses, and T2 progeny produced up to 3.6-fold higher CTs than the T0 parent. The profile of small CT oligomers such as dimers and trimers was consistent in T0, T1, T2, and BC2 progeny and consisted predominantly of prodelphinidins (PD), with lesser amounts of procyanidins (PC) and mixed PC:PD oligomers.

Keywordsanthocyanins; condensed tannins; pasture bloat; R2R3MYB transcription factor; white clover
ANZSRC Field of Research 2020310803. Plant cell and molecular biology
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PubMed ID31241924
FunderMinistry for Primary Industries
Byline AffiliationsAgResearch, New Zealand
PGG Wrightson Seeds, New Zealand
Massey University, New Zealand
University of Southern Queensland
Plant and Food Research, New Zealand
Halcyon Bioconsulting, New Zealand
Grasslanz Technology, New Zealand
International Livestock Research Institute, Ethiopia
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