Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repens L.) Outcrossed with High Anthocyanin Lines
Article
Roldan, Marissa B., Cousins, Greig, Fraser, Karl, Hancock, Kerry R., Collette, Vern, Demmer, Jerome, Woodfield, Derek R., Caradus, John R., Jones, Chris and Voisey, Christine R.. 2020. "Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repens L.) Outcrossed with High Anthocyanin Lines." Journal of Agricultural and Food Chemistry. 68 (10), pp. 2927-2939. https://doi.org/10.1021/acs.jafc.9b01185
| Article Title | Elevation of Condensed Tannins in the Leaves of Ta-MYB14-1 White Clover (Trifolium repens L.) Outcrossed with High Anthocyanin Lines |
|---|---|
| ERA Journal ID | 1349 |
| Article Category | Article |
| Authors | Roldan, Marissa B., Cousins, Greig, Fraser, Karl, Hancock, Kerry R., Collette, Vern, Demmer, Jerome, Woodfield, Derek R., Caradus, John R., Jones, Chris and Voisey, Christine R. |
| Journal Title | Journal of Agricultural and Food Chemistry |
| Journal Citation | 68 (10), pp. 2927-2939 |
| Year | 2020 |
| Publisher | American Chemical Society |
| Place of Publication | United States |
| ISSN | 0021-8561 |
| 1520-5118 | |
| Digital Object Identifier (DOI) | https://doi.org/10.1021/acs.jafc.9b01185 |
| Web Address (URL) | https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.9b01185 |
| Abstract | Condensed tannins (CT) are highly desirable in forage as they sequester dietary protein and reduce bloat and methane emissions in ruminants. However, the widely used forage legume white clover (Trifolium repens) only produces CTs in flowers and trichomes and at levels too low to achieve therapeutic effects. Genetic transformation with transcription factor Ta-MYB14-1 from Trifolium arvense was effective in inducing CTs to 0.6% of leaf dry matter. CT synthesis has been elevated further by crossing the primary white clover transgenic line with wild type genotypes producing the related phenylpropanoids, anthocyanins. CT levels in leaves were highest under the anthocyanin leaf marks associated with the "red midrib" trait; however, there was no evidence for CT accumulation in leaf sections with the "red V" anthocyanin marking. Ta-MYB14-1 was stably inherited in two generations of crosses, and T2 progeny produced up to 3.6-fold higher CTs than the T0 parent. The profile of small CT oligomers such as dimers and trimers was consistent in T0, T1, T2, and BC2 progeny and consisted predominantly of prodelphinidins (PD), with lesser amounts of procyanidins (PC) and mixed PC:PD oligomers. |
| Keywords | anthocyanins; condensed tannins; pasture bloat; R2R3MYB transcription factor; white clover |
| ANZSRC Field of Research 2020 | 310803. Plant cell and molecular biology |
| Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
| PubMed ID | 31241924 |
| Funder | Ministry for Primary Industries |
| Byline Affiliations | AgResearch, New Zealand |
| PGG Wrightson Seeds, New Zealand | |
| Massey University, New Zealand | |
| University of Southern Queensland | |
| Plant and Food Research, New Zealand | |
| Halcyon Bioconsulting, New Zealand | |
| Grasslanz Technology, New Zealand | |
| International Livestock Research Institute, Ethiopia |
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