Expression of the R2R3-MYB transcription factor TaMYB14 from Trifolium arvense Activates proanthocyanidin biosynthesis in the legumes Trifolium repens and Medicago sativa

Article


Hancock, Kerry R., Collette, Vern, Fraser, Karl, Greig, Margaret, Xue, Hong, Richardson, Kim, Jones, Chris and Rasmussen, Susanne. 2012. "Expression of the R2R3-MYB transcription factor TaMYB14 from Trifolium arvense Activates proanthocyanidin biosynthesis in the legumes Trifolium repens and Medicago sativa." Plant Physiology. 159 (3), pp. 1204-1220. https://doi.org/10.1104/pp.112.195420
Article Title

Expression of the R2R3-MYB transcription factor TaMYB14 from Trifolium arvense Activates proanthocyanidin biosynthesis in the legumes Trifolium repens and Medicago sativa

ERA Journal ID2653
Article CategoryArticle
AuthorsHancock, Kerry R., Collette, Vern, Fraser, Karl, Greig, Margaret, Xue, Hong, Richardson, Kim, Jones, Chris and Rasmussen, Susanne
Journal TitlePlant Physiology
Journal Citation159 (3), pp. 1204-1220
Number of Pages17
Year2012
ISSN0032-0889
1532-2548
Digital Object Identifier (DOI)https://doi.org/10.1104/pp.112.195420
Web Address (URL)https://academic.oup.com/plphys/article/159/3/1204/6109326
Abstract

Proanthocyanidins (PAs) are oligomeric flavonoids and one group of end products of the phenylpropanoid pathway. PAs have been reported to be beneficial for human and animal health and are particularly important in pastoral agricultural systems for improved animal production and reduced greenhouse gas emissions. However, the main forage legumes grown in these systems, such as Trifolium repens and Medicago sativa, do not contain any substantial amounts of PAs in leaves. We have identified from the foliar PA-accumulating legume Trifolium arvense an R2R3-MYB transcription factor, TaMYB14, and provide evidence that this transcription factor is involved in the regulation of PA biosynthesis in legumes. TaMYB14 expression is necessary and sufficient to up-regulate late steps of the phenylpropanoid pathway and to induce PA biosynthesis. RNA interference silencing of TaMYB14 resulted in almost complete cessation of PA biosynthesis in T. arvense, whereas Nicotiana tabacum, M. sativa, and T. repens plants constitutively expressing TaMYB14 synthesized and accumulated PAs in leaves up to 1.8% dry matter. Targeted liquid chromatography-multistage tandem mass spectrometry analysis identified foliar PAs up to degree of polymerization 6 in leaf extracts. Hence, genetically modified M. sativa and T. repens plants expressing TaMYB14 provide a viable option for improving animal health and mitigating the negative environmental impacts of pastoral animal production systems. © 2012 American Society of Plant Biologists. All Rights Reserved.

KeywordsPlant; Proanthocyanidins
ANZSRC Field of Research 2020300103. Agricultural molecular engineering of nucleic acids and proteins
Byline AffiliationsAgResearch, New Zealand
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