An integrated genetic linkage map for white clover (Trifolium repens L.) with alignment to Medicago

Article


Griffiths, Andrew G, Barrett, Brent A, Simon, Deborah, Khan, Anar K, Bickerstaff, Paul, Anderson, Craig B, Franzmayr, Benjamin K, Hancock Kerry R. and Jones, Chris S. 2013. "An integrated genetic linkage map for white clover (Trifolium repens L.) with alignment to Medicago." BMC Genomics. 14 (1), pp. 1-17. https://doi.org/10.1186/1471-2164-14-388
Article Title

An integrated genetic linkage map for white clover (Trifolium repens L.) with alignment to Medicago

ERA Journal ID2363
Article CategoryArticle
AuthorsGriffiths, Andrew G, Barrett, Brent A, Simon, Deborah, Khan, Anar K, Bickerstaff, Paul, Anderson, Craig B, Franzmayr, Benjamin K, Hancock Kerry R. and Jones, Chris S
Journal TitleBMC Genomics
Journal Citation14 (1), pp. 1-17
Article Number388
Number of Pages17
Year2013
PublisherBioMed Central Ltd.
Place of PublicationUnited Kingdom
ISSN1471-2164
Digital Object Identifier (DOI)https://doi.org/10.1186/1471-2164-14-388
Web Address (URL)https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-14-388
Abstract

Background: White clover (Trifolium repens L.) is a temperate forage legume with an allotetraploid genome (2n=4×=32) estimated at 1093 Mb. Several linkage maps of various sizes, marker sources and completeness are available, however, no integrated map and marker set has explored consistency of linkage analysis among unrelated mapping populations. Such integrative analysis requires tools for homoeologue matching among populations. Development of these tools provides for a consistent framework map of the white clover genome, and facilitates in silico alignment with the model forage legume, Medicago truncatula.Results: This is the first report of integration of independent linkage maps in white clover, and adds to the literature on methyl filtered GeneThresher®-derived microsatellite (simple sequence repeat; SSR) markers for linkage mapping. Gene-targeted SSR markers were discovered in a GeneThresher® (TrGT) methyl-filtered database of 364,539 sequences, which yielded 15,647 SSR arrays. Primers were designed for 4,038 arrays and of these, 465 TrGT-SSR markers were used for parental consensus genetic linkage analysis in an F1 mapping population (MP2). This was merged with an EST-SSR consensus genetic map of an independent population (MP1), using markers to match homoeologues and develop a multi-population integrated map of the white clover genome. This integrated map (IM) includes 1109 loci based on 804 SSRs over 1274 cM, covering 97% of the genome at a moderate density of one locus per 1.2 cM. Eighteen candidate genes and one morphological marker were also placed on the IM. Despite being derived from disparate populations and marker sources, the component maps and the derived IM had consistent representations of the white clover genome for marker order and genetic length. In silico analysis at an E-value threshold of 1e-20 revealed substantial co-linearity with the Medicago truncatula genome, and indicates a translocation between T. repens groups 2 and 6 relative to M. truncatula.Conclusions: This integrated genetic linkage analysis provides a consistent and comprehensive linkage analysis of the white clover genome, with alignment to a model forage legume. Associated marker locus information, particularly the homoeologue-specific markers, offers a new resource for forage legume research to enable genetic analysis and improvement of this forage and grassland species. © 2013 Griffiths et al.; licensee BioMed Central Ltd.

KeywordsAllotetraploid; GeneThresher®; Homoeologue; Legume; Linkage; Medicago truncatula; Microsatellite; SSR; Synteny; Trifolium repens
ANZSRC Field of Research 2020300406. Crop and pasture improvement (incl. selection and breeding)
PubMed ID23758831
FunderFoundation for Research, Science and Technology
Byline AffiliationsAgResearch, New Zealand
Pastoral Genomics, New Zealand
Landcorp Farming, New Zealand
Infoics, New Zealand
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