Characterization of Proanthocyanidins from Seeds of Perennial Ryegrass (Lolium perenne L.) and Tall Fescue (Festuca arundinacea) by Liquid Chromatography-Mass Spectrometry

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Fraser, Karl, Collette, Vern and Hancock Kerry R.. 2016. "Characterization of Proanthocyanidins from Seeds of Perennial Ryegrass (Lolium perenne L.) and Tall Fescue (Festuca arundinacea) by Liquid Chromatography-Mass Spectrometry." Journal of Agricultural and Food Chemistry. 64 (35), pp. 6676-6684. https://doi.org/10.1021/acs.jafc.6b02563
Article Title

Characterization of Proanthocyanidins from Seeds of Perennial Ryegrass (Lolium perenne L.) and Tall Fescue (Festuca arundinacea) by Liquid Chromatography-Mass Spectrometry

ERA Journal ID1349
Article CategoryArticle
AuthorsFraser, Karl, Collette, Vern and Hancock Kerry R.
Journal TitleJournal of Agricultural and Food Chemistry
Journal Citation64 (35), pp. 6676-6684
Number of Pages9
Year2016
PublisherAmerican Chemical Society
Place of PublicationUnited States
ISSN0021-8561
1520-5118
Digital Object Identifier (DOI)https://doi.org/10.1021/acs.jafc.6b02563
Web Address (URL)https://pubs.acs.org/doi/10.1021/acs.jafc.6b02563
Abstract

Perennial ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea) are forage species of the grass family (Poaceae) that are key components of temperate pasture-based agricultural systems. Proanthocyanidins (PAs) are oligomeric flavonoids that, when provided as part of a farm animal's diet, have been reported to improve animal production and health. Up to now, forage grasses have been deemed not to produce PAs. This paper reports for the first time the detection of polymerized PAs in aqueous methanolic extracts of seed tissue of both perennial ryegrass and tall fescue, using LC-MS/MS. We have determined the structure of the PAs to be trans-flavan-3-ol-based, consisting predominately of afzelechin and catechin and linked primarily by B-type bonds. Investigations into the leaf tissue of both species failed to detect any PAs. This discovery opens the possibility of using genetic engineering tools to achieve tannin accumulation in leaf tissue of perennial ryegrass and tall fescue.

Keywordsafzelechin; condensed tannins; electrospray mass spectrometry; Festuca arundinacea; forage grasses; LC-MS/MS; Lolium perenne; oligomers; perennial ryegrass; proanthocyanidin; procyanidins; propelargonidins; tall fescue
ANZSRC Field of Research 2020300499. Crop and pasture production not elsewhere classified
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PubMed ID27532250
Byline AffiliationsAgResearch, New Zealand
Office of Research
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