Genetic mapping of gray leaf spot (GLS) resistance genes in maize
Article
Article Title | Genetic mapping of gray leaf spot (GLS) resistance genes in maize |
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ERA Journal ID | 2411 |
Article Category | Article |
Authors | Lehmensiek, A., Esterhuizen, A. M., Van Staden, D., Nelson, S. W. and Retief, A. E. |
Journal Title | Theoretical and Applied Genetics: international journal of plant breeding research |
Journal Citation | 103 (5), pp. 797-803 |
Number of Pages | 7 |
Year | Oct 2001 |
Publisher | Springer |
Place of Publication | Germany |
ISSN | 0040-5752 |
1432-2242 | |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s001220100599 |
Web Address (URL) | https://link.springer.com/article/10.1007/s001220100599 |
Abstract | Bulked segregant analysis was used to identify amplified fragment length polymorphism markers (AFLPs) linked to quantitative trait loci (QTLs) involved in the resistance to gray leaf spot (GLS) in maize. By using ten AFLP primer combinations 11 polymorphic markers were identified and converted to sequence-specific PCR markers. Five of the 11 converted AFLPs were linked to three GLS resistance QTLs. The markers were mapped to maize chromosomes 1, 3 and 5 using existing linkage maps of two commercially available recombinant inbred-line populations. Converted restriction fragment length polymorphism markers and microsatellite markers were used to obtain a more-precise localization for the detected QTLs. The QTL on chromosome 1 was localized in bin 1.05/06 and had a LOD score of 21. A variance of 37% was explained by the QTL. Two peaks were visible on chromosome 5, one was localized in bin 5.03/04 and the other in bin 5.05/06. Both peaks had a LOD score of 5, and 11% of the variance was explained by the QTLs. A variance of 8-10% was explained by the QTL on chromosome 3 (bin 3.04). The consistency of the QTLs was tested across two F2 populations planted in consecutive years. |
Keywords | gray leaf spot; maize; bulked segregant analysis; AFLPs; QTL analysis |
Public Notes | There are no files associated with this item. |
Byline Affiliations | Stellenbosch University, South Africa |
No affiliation |
https://research.usq.edu.au/item/w4489/genetic-mapping-of-gray-leaf-spot-gls-resistance-genes-in-maize
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