Analysis and manipulation of odor-plume structure from a piezo-electric pheromone release system and its effects on upwind flight of male moths to pheromone
Presentation
Girling, R.D. and Cardé, R.T.. 2006. "Analysis and manipulation of odor-plume structure from a piezo-electric pheromone release system and its effects on upwind flight of male moths to pheromone." International Society of Chemical Ecology Annual Meeting 2006. Barcelona, Spain 15 - 19 Jul 2006 Spain.
Paper/Presentation Title | Analysis and manipulation of odor-plume structure from a piezo-electric pheromone release system and its effects on upwind flight of male moths to pheromone |
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Presentation Type | Presentation |
Authors | Girling, R.D. and Cardé, R.T. |
Journal Citation | p. 150 |
Number of Pages | 1 |
Year | 2006 |
Place of Publication | Spain |
Web Address (URL) of Paper | https://chemecol.org/programs/2006.pdf |
Web Address (URL) of Conference Proceedings | https://www.chemecol.org/pastmeetings.shtml |
Conference/Event | International Society of Chemical Ecology Annual Meeting 2006 |
Event Details | International Society of Chemical Ecology Annual Meeting 2006 Event Date 15 to end of 19 Jul 2006 Event Location Barcelona, Spain |
Abstract | Piezo-electric sprayers for pheromone release enable the precise release of chemical stimuli as an aerosol, from a solution. These systems consists of a drawn glass capillary tip, with an opening of 10-50 µm internal diameter, which is vibrated at ultrasonic frequency (~120 kHz) by a piezo-ceramic disc. This disperses the solution in micro-droplets, which results in an aerosol which in turn evaporates completely within 5 cm of the tip. Plume structure has been demonstrated to be an important factor in the orientation of moths to an odor source. This sprayer system is routinely set-up with the downwind end shielded from insects by a protective mesh, but what effect this has on the plume structure produced by the system or in fact what plume structure the system produces while uncovered has not previously been documented. We investigated the odor plume structure of a typical piezo-electric sprayer system, set up to release ethanol, in a wind tunnel using a fast response mini-photoionizaton detector. We recorded the plume structure of four different sprayer configurations: the sprayer alone, a 5 cm circular upwind baffle, a 1.6mm steel mesh shield, and a 3.2mm steel mesh shield. We measured a 12 mm x 12 mm core at the center of the plume, and both a horizontal and vertical cross-section of the plume, all at 100 mm, 200 mm and 400 mm downwind of the release point. Marked differences in plume structure were found between all configurations in terms of conditional relative mean concentration, intermittency, ratio of peak concentration to conditional mean concentration, and size of the plume. We then measured the flight responses of the almond moth, Cadra cautella, to the different sprayer configurations, to identify whether these differences in plume structure had any effects on moth flights. Differences in plume structure between the sprayer configurations resulted in varying flight responses. This study provides further evidence that pheromone plume structure has effects of moth orientation behavior and demonstrates that care is needed when setting up experiments using a piezo-electric release system to ensure the optimal conditions for behavioral observations. |
ANZSRC Field of Research 2020 | 300804. Horticultural crop protection (incl. pests, diseases and weeds) |
310901. Animal behaviour | |
310301. Behavioural ecology | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | University of California, United States |
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