Heterogeneity of alpha-cardiac myosin heavy chains in a small marsupial, Antechinus flavipes, and the effect of hypothyroidism on its ventricular myosins
Article
Article Title | Heterogeneity of alpha-cardiac myosin heavy chains in a small marsupial, Antechinus flavipes, and the effect of hypothyroidism on its ventricular myosins |
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ERA Journal ID | 3164 |
Article Category | Article |
Authors | Hoh, Joseph F. Y. (Author), Withers, Kerry W. (Author) and Zhong, Wendy W. H. (Author) |
Journal Title | Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology |
Journal Citation | 178 (3), pp. 279-284 |
Number of Pages | 6 |
Year | 2008 |
Place of Publication | Heidelberg, Germany |
ISSN | 0174-1578 |
1432-136X | |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s00360-007-0220-1 |
Abstract | The effect of drug-induced hypothyroidism on ventricular myosin gene expression was explored in a small marsupial, Antechinus flavipes. Pyrophosphate gel electrophoresis, SDS-PAGE and western blotting were used to analyse changes in native myosin isoforms and myosin heavy chains (MyHCs) in response to hypothyroidism. In some animals, five instead of the normal three native myosin components were found: V1a, V1b, V1c, V2 and V3, in order of decreasing mobility. In western blots, V1a, V1b, and V1c reacted with anti-a-MyHC antibody, but not with anti-B-MyHC, whereas V2 and V3 reacted with anti-B-MyHC antibody. SDS-PAGE of the unusual ventricular myosins revealed three MyHC isoforms, two of which bound anti-a-MyHC antibody while the third bound anti-B-MyHC antibody. |
Keywords | marsupial; Antechinus; cardiac muscle; myosin heavy chain; thyroid hormone |
ANZSRC Field of Research 2020 | 310912. Comparative physiology |
320101. Cardiology (incl. cardiovascular diseases) | |
320506. Medical biochemistry - proteins and peptides (incl. medical proteomics) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | University of Sydney |
Centre for Systems Biology |
https://research.usq.edu.au/item/9zq73/heterogeneity-of-alpha-cardiac-myosin-heavy-chains-in-a-small-marsupial-antechinus-flavipes-and-the-effect-of-hypothyroidism-on-its-ventricular-myosins
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