Skeletal muscle mitochondria as a source of non-shivering thermogenesis in the marsupial Anthechinus flavipes

Paper

Jastroch, Martin, Withers, Kerry and Klingenspor, Martin. 2005. "Skeletal muscle mitochondria as a source of non-shivering thermogenesis in the marsupial Anthechinus flavipes." Cree, Alison (ed.) 22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005). Dunedin, New Zealand 09 - 11 Dec 2005 New Zealand.
Paper/Presentation Title

Skeletal muscle mitochondria as a source of non-shivering thermogenesis in the marsupial Anthechinus flavipes

Presentation TypePaper
AuthorsJastroch, Martin (Author), Withers, Kerry (Author) and Klingenspor, Martin (Author)
EditorsCree, Alison
Journal or Proceedings TitleANZSCPB Proceedings, v. 22
Journal Citation22, p. 58
Number of Pages1
Year2005
Place of PublicationNew Zealand
ISBN0473107198
Conference/Event22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005)
Event Details
22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005)
Event Date
09 to end of 11 Dec 2005
Event Location
Dunedin, New Zealand
Abstract

The source of non-shivering thermogenesis (NST) in non-placental mammals is not known as they apparently lack
brown adipose tissue (BAT) and uncoupling protein 1 (UCP1). In placental mammals, UCP1 mediates uncoupled
respiration in BAT to generate heat. A thermogenic role of UCP3 and the adenine nucleotide transporter (ANT)
has been debated for bird skeletal muscle. It has been established that 4-hydroxy-2-trans-nonenal (HNE) induces
uncoupling activity by UCPs and/or ANT. Whether UCP or ANT is activated can be distinguished by GDPinhibition
of UCPs or carboxyatractyloside (CAT)-inhibition of the ANT. Here, we investigate the role of proton
conductance, UCP3 and ANT in skeletal muscle mitochondria during cold exposure of marsupials. We acclimated
the yellow-footed Antechinus (A. flavipes) to either 10° C or 24°C for 3-4 weeks and measured proton leakage in
isolated skeletal muscle mitochondria. Whereas basal proton conductance was unchanged between different
acclimation temperatures, a higher inducible proton conductance by HNE was observed in the cold acclimated
group. Inhibition by CAT indicated uncoupling activity by the ANT. Pertaining to UCP3, GDP-inhibition could
not be observed and might have been missed by applying assay conditions routinely used for rodents. Regarding
the dominant contribution of skeletal muscle to body mass, we demonstrate a potential role of their mitochondria
in adaptive thermogenesis. The capacity of proton leakage is increased in the cold and can be regulated by an ANTdependent mechanism. The present study provides no evidence for the involvement of UCP3 in adaptive
thermogenesis of marsupials.

Keywordsnon-shivering thermogenesis; marsupials
ANZSRC Field of Research 2020300902. Veterinary anatomy and physiology
Public Notes

Abstract 80. Conference publication consists of only the abstracts of papers presented at the conference.

Byline AffiliationsPhilipps-University Marburg, Germany
Department of Biological and Physical Sciences
Technical University of Munich, Germany
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