Uncoupling protein 2 and 3 in marsupials: identification, phylogeny and gene expression in response to cold and fasting in Antechinus flavipes

Article


Jastroch, Martin, Withers, Kerry and Klingenspor, Martin. 2004. "Uncoupling protein 2 and 3 in marsupials: identification, phylogeny and gene expression in response to cold and fasting in Antechinus flavipes." Physiological Genomics. 17 (2), pp. 130-139. https://doi.org/10.1152/physiolgenomics.00165.2003
Article Title

Uncoupling protein 2 and 3 in marsupials: identification, phylogeny and gene expression in response to cold and fasting in Antechinus flavipes

ERA Journal ID14629
Article CategoryArticle
AuthorsJastroch, Martin (Author), Withers, Kerry (Author) and Klingenspor, Martin (Author)
Journal TitlePhysiological Genomics
Journal Citation17 (2), pp. 130-139
Number of Pages10
Year2004
Place of PublicationUnited States
ISSN1094-8341
1531-2267
Digital Object Identifier (DOI)https://doi.org/10.1152/physiolgenomics.00165.2003
Web Address (URL)https://journals.physiology.org/doi/full/10.1152/physiolgenomics.00165.2003
Abstract

We searched for the presence of uncoupling protein genes so far unknown in marsupials and monotremes and identified uncoupling protein 2 (UCP2) and UCP3 full-length cDNAs in libraries constructed from the marsupials Antechinus flavipes and Sminthopsis macroura. Marsupial
UCP2 is 89–90% identical to rodent UCP2, whereas UCP3
exhibits 80% identity to mouse UCP3. A phylogenetic tree including all known UCPs positions the novel marsupial UCP2 and UCP3 at the base of the mammalian orthologs. In the 5-untranslated region of UCP2 a second open reading frame encoding for a 36-amino acid peptide was identified which is highly conserved in all vertebrate UCP2 transcripts. Analysis of tissue specificity in A. flavipes with homologous cDNA probes revealed ubiquitous presence of UCP2 mRNA and striated muscle specificity of UCP3 mRNA resembling
the known expression pattern in rodents. Neither UCP2 nor UCP3 gene expression was stimulated in adipose tissue and skeletal muscle of cold exposed A. flavipes. However, UCP3 mRNA expression was upregulated 6-fold in heart and 2.5-fold in skeletal muscle as reported for rodents in response to fasting. Furthermore, UCP3 mRNA seems to be coregulated with PDK4 mRNA, indicating a relation to enhanced
lipid metabolism. In contrast, UCP2 gene expression was not
regulated in response to fasting in adipose tissue and skeletal muscle but was diminished in the lung and increased in adipose tissue. Taken together, the sequence analysis, tissue specificity and physiological
regulation suggest a conserved function of UCP2 and UCP3 during 130 million years of mammalian evolution.

KeywordsSminthopsis macroura; Tachyglossus aculeatus; pyruvate dehydrogenase 4; monotremes
ANZSRC Field of Research 2020310999. Zoology not elsewhere classified
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Byline AffiliationsPhilipps-University Marburg, Germany
Department of Biological and Physical Sciences
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