Uncoupling protein 2 and 3 in marsupials: identification, phylogeny and gene expression in response to cold and fasting in Antechinus flavipes

Article

Jastroch, Martin, Withers, Kerry and Klingenspor, Martin. 2004. "Uncoupling protein 2 and 3 in marsupials: identification, phylogeny and gene expression in response to cold and fasting in Antechinus flavipes." Physiological Genomics. 17 (2), pp. 130-139. https://doi.org/10.1152/physiolgenomics.00165.2003

Article Title	Uncoupling protein 2 and 3 in marsupials: identification, phylogeny and gene expression in response to cold and fasting in Antechinus flavipes
ERA Journal ID	14629
Article Category	Article
Authors	Jastroch, Martin (Author), Withers, Kerry (Author) and Klingenspor, Martin (Author)
Journal Title	Physiological Genomics
Journal Citation	17 (2), pp. 130-139
Number of Pages	10
Year	2004
Publisher	American Physiological Society
Place of Publication	United States
ISSN	1094-8341
	1531-2267
Digital Object Identifier (DOI)	https://doi.org/10.1152/physiolgenomics.00165.2003
Web Address (URL)	https://journals.physiology.org/doi/full/10.1152/physiolgenomics.00165.2003
Abstract	We searched for the presence of uncoupling protein genes so far unknown in marsupials and monotremes and identified uncoupling protein 2 (UCP2) and UCP3 full-length cDNAs in libraries constructed from the marsupials Antechinus flavipes and Sminthopsis macroura. Marsupial UCP2 is 89–90% identical to rodent UCP2, whereas UCP3 exhibits 80% identity to mouse UCP3. A phylogenetic tree including all known UCPs positions the novel marsupial UCP2 and UCP3 at the base of the mammalian orthologs. In the 5-untranslated region of UCP2 a second open reading frame encoding for a 36-amino acid peptide was identified which is highly conserved in all vertebrate UCP2 transcripts. Analysis of tissue specificity in A. flavipes with homologous cDNA probes revealed ubiquitous presence of UCP2 mRNA and striated muscle specificity of UCP3 mRNA resembling the known expression pattern in rodents. Neither UCP2 nor UCP3 gene expression was stimulated in adipose tissue and skeletal muscle of cold exposed A. flavipes. However, UCP3 mRNA expression was upregulated 6-fold in heart and 2.5-fold in skeletal muscle as reported for rodents in response to fasting. Furthermore, UCP3 mRNA seems to be coregulated with PDK4 mRNA, indicating a relation to enhanced lipid metabolism. In contrast, UCP2 gene expression was not regulated in response to fasting in adipose tissue and skeletal muscle but was diminished in the lung and increased in adipose tissue. Taken together, the sequence analysis, tissue specificity and physiological regulation suggest a conserved function of UCP2 and UCP3 during 130 million years of mammalian evolution.
Keywords	Sminthopsis macroura; Tachyglossus aculeatus; pyruvate dehydrogenase 4; monotremes
ANZSRC Field of Research 2020	310999. Zoology not elsewhere classified
Public Notes	Files associated with this item cannot be displayed due to copyright restrictions.
Byline Affiliations	Philipps-University Marburg, Germany
	Department of Biological and Physical Sciences

Permalink -

https://research.usq.edu.au/item/9y5vy/uncoupling-protein-2-and-3-in-marsupials-identification-phylogeny-and-gene-expression-in-response-to-cold-and-fasting-in-antechinus-flavipes

Download files

Export as

Related outputs

Bat fat: body composition analysis by total body electrical conductivity in northern long-eared bats

Tomasi, Tom, Withers, Kerry W. and Day, Katie M.. 2007. "Bat fat: body composition analysis by total body electrical conductivity in northern long-eared bats." Cook, J., Dunnum, J., Gannon, B. and Yates, T. (ed.) 87th Annual Meeting of the American Society of Mammalogists . Albuquerque, United States 06 - 10 Jun 2007 Lawrence, KS. United States.

Chronic l-arginine treatment improves metabolic, cardiovascular and liver complications in diet-induced obesity in rats

Alam, Md. Ashraful, Kauter, Kathleen, Withers, Kerry, Sernia, Conrad and Brown, Lindsay. 2013. "Chronic l-arginine treatment improves metabolic, cardiovascular and liver complications in diet-induced obesity in rats." Food and Function. 4 (1), pp. 83-91. https://doi.org/10.1039/c2fo30096f

Phylogenetic differences of mammalian basal metabolic rate are not explained by mitochondrial basal proton leak

Polymeropoulos, E. T., Heldmaier, G., Frappell, P. B., McAllan, B. M., Withers, K. W., Klingenspor, M., White, C. R. and Jastroch, M.. 2012. "Phylogenetic differences of mammalian basal metabolic rate are not explained by mitochondrial basal proton leak." Proceedings of the Royal Society B: Biological Sciences. 279 (1726), pp. 185-193. https://doi.org/10.1098/rspb.2011.0881

Determining the age of adult wild dogs (Canis lupus dingo, C. l. domesticus and their hybrids): I. Pulp cavity/tooth width ratios

Kershaw, K., Allen, L., Lisle, A. and Withers, K.. 2005. "Determining the age of adult wild dogs (Canis lupus dingo, C. l. domesticus and their hybrids): I. Pulp cavity/tooth width ratios." Wildlife Research. 32 (6), pp. 581-585. https://doi.org/10.1035-3712/05/060581

Measuring the density of dingo teeth with machine vision

Billingsley, John and Withers, Kerry. 2004. "Measuring the density of dingo teeth with machine vision." Sensor Review. 24 (4), pp. 361-363. https://doi.org/10.1108/02602280410558386

Behaviour of juvenile antechinus flavipes: a preliminary study

Wollenhaupt, Anja, Withers, Kerry and Billingsley, John. 2009. "Behaviour of juvenile antechinus flavipes: a preliminary study." 26th Annual Meeting of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2009). Geelong, Australia 27 - 29 Nov 2009 Perth, Australia.

Strain specific differences in parameters of energy balance are related to the onset of diet-induced obesity in young male mice

Hesse, Deike, Dunn, Mark, Billingsley, John, Withers, Kerry, Klingenspor, Martin and Rozman, Jan. 2007. "Strain specific differences in parameters of energy balance are related to the onset of diet-induced obesity in young male mice." 15th European Congress on Obesity. Budapest, Hungary 22 - 25 Apr 2007 London, United Kingdom. https://doi.org/10.1038/sj.ijo.0803597

Small mammal habitat preferences in Ravensbourne National Park, south-east Queensland

Page, Skye, Le Brocque, Andrew F. and Withers, Kerry. 2002. "Small mammal habitat preferences in Ravensbourne National Park, south-east Queensland." 2002 Conference of the Ecological Society of Australia (ESA 2002). Cairns, Australia 02 - 06 Dec 2002 Brisbane, Queensland, Australia.

Habitat preferences of small ground-dwelling mammals

Page, Skye, Le Brocque, Andrew F. and Withers, Kerry. 2003. "Habitat preferences of small ground-dwelling mammals ." Partners in Parks: 2003 Collaborative Research Forum, Queensland Parks and Wildlife Service . Brisbane, Australia 02 Oct 2003 Brisbane, Australia.

Behavioural mechanisms affecting energy regulation in mice prone or resistant to diet-induced obesity

Hesse, Deike, Dunn, Mark, Heldmaier, Gerhard, Klingenspor, Martin and Rozman, Jan. 2010. "Behavioural mechanisms affecting energy regulation in mice prone or resistant to diet-induced obesity." Physiology and Behavior. 99 (3), pp. 370-380. https://doi.org/10.1016/j.physbeh.2009.12.001

Mitochondrial proton conductance in skeletal muscle of a cold-exposed marsupial, Antechinus flavipes, is unlikely to be involved in adaptive non-shivering thermogenesis but displays increased sensitivity towards carbon-centered radicals

Jastroch, Martin, Withers, Kerry, Stoehr, Sigrid and Klingenspor, Martin. 2009. "Mitochondrial proton conductance in skeletal muscle of a cold-exposed marsupial, Antechinus flavipes, is unlikely to be involved in adaptive non-shivering thermogenesis but displays increased sensitivity towards carbon-centered radicals." Physiological and Biochemical Zoology. 82 (5), pp. 447-454. https://doi.org/10.1086/603631

Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, antechinus flavipes

Zhong, Wendy W. H., Withers, Kerry W. and Hoh, Joseph F. Y.. 2010. "Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, antechinus flavipes." Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology. 180 (4), pp. 531-544. https://doi.org/10.1007/s00360-009-0431-8

Marsupial uncoupling protein 1 sheds light on the evolution of mammalian nonshivering thermogenesis

Jastroch, M., Withers, K. W., Taudien, S., Frappell, P. B., Helwig, M., Fromme, T., Hirschberg, V., Heldmaier, G., McAllan, B. M., Firth, B. T., Burmester, T., Platzer, M. and Klingenspor, M.. 2008. "Marsupial uncoupling protein 1 sheds light on the evolution of mammalian nonshivering thermogenesis." Physiological Genomics. 32 (2), pp. 161-169. https://doi.org/10.1152/physiolgenomics.00183.2007

The effects of fasting and cold exposure on metabolic rate and mitochondrial proton leak in liver and skeletal muscle of an amphibian, the cane toad Bufo marinus

Trzcionka, M., Withers, K. W., Klingenspor, M. and Jastroch, M.. 2008. "The effects of fasting and cold exposure on metabolic rate and mitochondrial proton leak in liver and skeletal muscle of an amphibian, the cane toad Bufo marinus." The Journal of Experimental Biology. 211 (12), pp. 1911-1918. https://doi.org/10.1242/jeb.016519

Heterogeneity of alpha-cardiac myosin heavy chains in a small marsupial, Antechinus flavipes, and the effect of hypothyroidism on its ventricular myosins

Hoh, Joseph F. Y., Withers, Kerry W. and Zhong, Wendy W. H.. 2008. "Heterogeneity of alpha-cardiac myosin heavy chains in a small marsupial, Antechinus flavipes, and the effect of hypothyroidism on its ventricular myosins." Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology. 178 (3), pp. 279-284. https://doi.org/10.1007/s00360-007-0220-1

Maintaining the ancient bunya tree (Araucaria bidwillii Hook.) - dispersal and mast years

Smith, I. R., Withers, Kerry and Billingsley, John. 2007. "Maintaining the ancient bunya tree (Araucaria bidwillii Hook.) - dispersal and mast years." Hill, Bill (ed.) 5th International Southern Connection Conference. Adelaide, Australia 21 - 25 Jan 2007 Adelaide, Australia.

Application of machine vision to determine the density of dingo teeth

Withers, Kerry W. and Billingsley, J.. 2004. "Application of machine vision to determine the density of dingo teeth." Buttemer, W. A., Else, P. L., Hulbert, A. J., Faulks, S. C. and Turner, N. (ed.) ANZSCPB 2004: 21st Annual Meeting of Australian and New Zealand Society for Comparative Physiology and Biochemistry. Wollongong, Australia 09 - 12 Dec 2004 Wollongong, Australia.

Pesticide effects on endocrine function in hibernating 13-lined ground squirrels

Perry, Jean, Tomasi, Thomas E. and Withers, Kerry. 2003. "Pesticide effects on endocrine function in hibernating 13-lined ground squirrels." Missouri Life Sciences Week 2003. Missouri, United States 03 - 07 Mar 2003 Missouri, MO. United States.

Pesticide effects on body temperature of torpid/hibernating rodents (Peromyscus leucopus and Spermophilus tridecemlineatus)

Tomasi, Thomas E., Elsken-Lacy, Peta, Perry, Jean A. and Withers, Kerry. 2004. "Pesticide effects on body temperature of torpid/hibernating rodents (Peromyscus leucopus and Spermophilus tridecemlineatus)." Barnes, Brian M. and Carey, Hannah V. (ed.) 12th International Hibernation Symposium. Alaska, USA 25 - 31 Jul 2004 Fairbanks,Alaska, USA.

A quest for the origin of mammal uncoupling proteins

Jastroch, Martin, Stoehr, Sigrid, Withers, Kerry and Klingenspor, Martin. 2004. "A quest for the origin of mammal uncoupling proteins." Barnes, Brian M. and Carey, Hannah V. (ed.) 12th International Hibernation Symposium. Alaska, USA 25 - 31 Jul 2004 Fairbanks, Alaska, USA.

The role of native rodents in seed dispersal and seed predation of the Bunya Pine (Araucaria bidwillii)

Smith, I. R., Withers, Kerry and Billingsley, John. 2005. "The role of native rodents in seed dispersal and seed predation of the Bunya Pine (Araucaria bidwillii)." Cree, Alison (ed.) 22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005). Dunedin, New Zealand 09 - 11 Dec 2005 New Zealand.

Use of pulp cavity/tooth width ratios to determine the age of adult wild dogs (Canis lupus dingo, C. l. domesticus and their hybrids)

Kershaw, K., Allen, L., Lisle, A. and Withers, K.. 2005. "Use of pulp cavity/tooth width ratios to determine the age of adult wild dogs (Canis lupus dingo, C. l. domesticus and their hybrids)." Cree, Alison (ed.) 22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005). Dunedin, New Zealand 09 - 11 Dec 2005 New Zealand.

Skeletal muscle mitochondria as a source of non-shivering thermogenesis in the marsupial Anthechinus flavipes

Jastroch, Martin, Withers, Kerry and Klingenspor, Martin. 2005. "Skeletal muscle mitochondria as a source of non-shivering thermogenesis in the marsupial Anthechinus flavipes." Cree, Alison (ed.) 22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005). Dunedin, New Zealand 09 - 11 Dec 2005 New Zealand.

Identifying reproductive state of the Julia Creek dunnart Sminthopsis douglasi by behavioural observations

Bjursell, A., Withers, K., Senior, G., Lundie-Jenkins, G. and Kennerley, P.. 2005. "Identifying reproductive state of the Julia Creek dunnart Sminthopsis douglasi by behavioural observations." Cree, Alison (ed.) 22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005). Dunedin, New Zealand 09 - 11 Dec 2005 New Zealand.

Application of machine vision technology to identification of oestrous in the Julia Creek dunnart (Sminthopsis douglasi)

Bjursell, A., Dunn, M., Withers, K., Senior, G., Lundie-Jenkins, G. and Billingsley, J.. 2005. "Application of machine vision technology to identification of oestrous in the Julia Creek dunnart (Sminthopsis douglasi)." Cree, Alison (ed.) 22nd Annual Conference of the Australian and New Zealand Society for Comparative Physiology and Biochemistry (ANZSCPB 2005). Dunedin, New Zealand 09 - 11 Dec 2005 New Zealand.

Torpor in the carnivorous marsupial Sminthopsis macroura: effects of food quality and food quantity

Withers, Kerry W., White, Debra H. and Billingsley, John. 2000. "Torpor in the carnivorous marsupial Sminthopsis macroura: effects of food quality and food quantity." Heldmaier, Gerhard, Klingenspor, Martin and Klaus, Susanne (ed.) 11th International Hibernation Symposium. Jungholz, Austria 13 - 18 Aug 2000 Berlin, Germany.