Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, antechinus flavipes

Article


Zhong, Wendy W. H., Withers, Kerry W. and Hoh, Joseph F. Y.. 2010. "Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, antechinus flavipes." Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology. 180 (4), pp. 531-544. https://doi.org/10.1007/s00360-009-0431-8
Article Title

Effects of hypothyroidism on myosin heavy chain composition and fibre types of fast skeletal muscles in a small marsupial, antechinus flavipes

ERA Journal ID3164
Article CategoryArticle
AuthorsZhong, Wendy W. H. (Author), Withers, Kerry W. (Author) and Hoh, Joseph F. Y. (Author)
Journal TitleJournal of Comparative Physiology B: biochemical, systemic, and environmental physiology
Journal Citation180 (4), pp. 531-544
Number of Pages14
Year2010
Place of PublicationHeidelberg, Germany
ISSN0174-1578
1432-136X
Digital Object Identifier (DOI)https://doi.org/10.1007/s00360-009-0431-8
Abstract

Effects of drug-induced hypothyroidism on myosin heavy chain (MyHC) content and fibre types of fast skeletal muscles were studied in a small marsupial, Antechinus flavipes. SDS-PAGE of MyHCs from the tibialis anterior and gastrocnemius revealed four isoforms, 2B, 2X, 2A and slow, in that order of decreasing abundance. After 5 weeks treatment with methimazole, the functionally fastest 2B MyHC significantly decreased, while 2X, 2A and slow MyHCs increased. Immunohistochemistry using monospecific antibodies to each of the four MyHCs revealed decreased 2b and 2x fibres, and increased 2a and hybrid fibres co-expressing two or three MyHCs. In the normally homogeneously fast superficial regions of these muscles, evenly distributed slow-staining fibres appeared, resembling the distribution of slow primary myotubes in fast muscles during development. Hybrid fibres containing 2A and slow MyHCs were virtually absent. These results are more detailed but broadly similar to the earlier studies on eutherians. We hypothesize that hypothyroidism essentially reverses the effects of thyroid hormone on MyHC gene expression of muscle fibres during myogenesis, which differ according to the developmental origin of the fibre: it induces slow MyHC expression in 2b fibres derived from fast primary myotubes, and shifts fast MyHC expression in fibres of secondary origin towards 2A, but not slow, MyHC.

Keywordsmarsupial; skeletal muscle; myosin; fibre types; hypothyroidism; immunohistochemistry
ANZSRC Field of Research 2020310103. Cell metabolism
320507. Metabolic medicine
310912. Comparative physiology
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Byline AffiliationsUniversity of Sydney
Centre for Systems Biology
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