Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan
Article
Article Title | Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan |
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ERA Journal ID | 4694 |
Article Category | Article |
Authors | Das, Suvendu (Author), Kar, Sandeep (Author), Jean, Jiin-Shuh (Author), Rathod, Jagat (Author), Chakraborty, Sukalyan (Author), Liu, Hsiao-Sheng (Author) and Bundschuh, Jochen (Author) |
Journal Title | Water Research |
Journal Citation | 47 (19), pp. 6983-6991 |
Number of Pages | 9 |
Year | 2013 |
Place of Publication | London, United Kingdom |
ISSN | 0043-1354 |
1879-2448 | |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.watres.2013.07.049 |
Abstract | The role of arsenite oxidizers in natural attenuation of arsenic pollution necessitates studies on their abundance and diversity in arsenic-contaminated aquifers. In this study, most probable number-polymerase chain reaction (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) was applied to monitor depth-wise abundance and diversity of aerobic arsenite oxidizers in arsenic-enriched groundwater of Beimen, southwestern Taiwan. The results revealed that the abundance of arsenite oxidizers ranged from 0.04 to 0.22, and the lowest ratio was observed in the most arsenic-enriched and comparatively more reduced groundwater (depth 200m) of Beimen 1. The highest ratio was observed in the less arsenic-enriched and less reduced groundwater (depth 60m) of Beimen 2B. DGGE profiles showed a shift in diversity of arsenite oxidizers, consisting of members of the Betaproteobacteria (61%), Alphaproteobacteria (28%) and Gammaproteobacteria (11%), depending on mainly arsenic concentration and redox level in groundwater. Groundwater with the lowest arsenic and highest dissolved oxygen at Beimen 2B harbored 78% of the arsenite oxidizers communities, while groundwater with the highest arsenic and lowest dissolved oxygen at Beimen 1 and Beimen-Jinhu harbored 17 and 22% of arsenite oxidizers communities, respectively. Pseudomonas sp. was found only in groundwater containing high arsenic at Beimen 1 and Beimen-Jinhu, while arsenite oxidizers belonging to Alpha- and Betaproteobacteria were dominated in groundwater containing low arsenic. |
Keywords | arsenite oxidizing bacteria; groundwater arsenic; MPN-PCR; PCR-DGGE |
ANZSRC Field of Research 2020 | 410304. Environmental biotechnology diagnostics (incl. biosensors) |
300201. Agricultural hydrology | |
400513. Water resources engineering | |
Public Notes | File reproduced in accordance with the copyright policy of the publisher/author. |
Byline Affiliations | National Cheng Kung University, Taiwan |
Maharaja Sayajirao University of Baroda, India | |
National Centre for Engineering in Agriculture | |
Institution of Origin | University of Southern Queensland |
https://research.usq.edu.au/item/q2686/depth-resolved-abundance-and-diversity-of-arsenite-oxidizing-bacteria-in-the-groundwater-of-beimen-a-blackfoot-disease-endemic-area-of-southwestern-taiwan
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