Phytoremediation and sequestration of soil metals using the CRISPR/Cas9 technology to modify plants: a review
Article
Article Title | Phytoremediation and sequestration of soil metals using the CRISPR/Cas9 technology to modify plants: a review |
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ERA Journal ID | 39950 |
Article Category | Article |
Authors | Bhattacharyya, Nirjhar, Anand, Uttpal, Kumar, Ravi, Ghorai, Mimosa, Aftab, Tariq, Jha, Niraj Kumar, Rajapaksha, Anushka Upamali, Bundschuh, Jochen, Bontempi, Elza and Dey, Abhijit |
Journal Title | Environmental Chemistry Letters |
Journal Citation | 21 (1), pp. 429-445 |
Number of Pages | 17 |
Year | 2023 |
Publisher | Springer |
Place of Publication | Germany |
ISSN | 1610-3653 |
1610-3661 | |
Digital Object Identifier (DOI) | https://doi.org/10.1007/s10311-022-01474-1 |
Web Address (URL) | https://link.springer.com/article/10.1007/s10311-022-01474-1 |
Abstract | Soil contamination by toxic metals is a major health issue that could be partly solved by using genetically-modified plants. For that, the recently developed technique of clustered regularly interspaced short palindromic repeats (CRISPR) has created a new dimension in genetic engineering. CRISPR was first found as a part of the adaptive immune system in bacteria and archaea, and further refined to generate targeted breaks in DNA in a broad range of organisms. Various DNA changes can take place during the cellular repair process. Many plants, including crops, have the potential to tolerate, stabilize, and transform both organic and metal contaminants and have been already modified using the CRISPR method. Furthermore, many genes necessary to increase the absorption and tolerance of metals have been identified. Thus, using CRISPR, target genes could be activated or repressed to optimize phytoremediation in plants. Here we review the CRISPR/Cas9 technology applied to phytoremediation and sequestration of metals in the soil environment. The availability of the genome sequence plays a critical role in the adaptation of the CRISPR-mediated genome editing to specific plants. CRISPR has demonstrated outstanding potential for genome editing. However, the outcome depends on the selected target site, Cas9/Cpf1 function, gRNA design, delivery systems, and the off-target effects that may restrict its efficacy. |
Keywords | Adenine base editors; CRISPR/Cas9; Cytidine base editors; Homology-directed repair; Non-homologous end joining; Phytoremediation |
ANZSRC Field of Research 2020 | 4199. Other environmental sciences |
Public Notes | File reproduced in accordance with the copyright policy of the publisher/author. |
Funder | Presidency University |
Byline Affiliations | Presidency University, India |
Ben-Gurion University of the Negev, Israel | |
University of Delhi, India | |
Aligarh Muslim University, India | |
Sharda University, India | |
Chandigarh University, India | |
Uttaranchal University, India | |
University of Sri Jayewardenepura, Sri Lanka | |
School of Engineering | |
University of Brescia, Italy | |
Library Services |
https://research.usq.edu.au/item/yy735/phytoremediation-and-sequestration-of-soil-metals-using-the-crispr-cas9-technology-to-modify-plants-a-review
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