Phenotypic and phylogenetic studies associated with the crucifer white leaf spot pathogen, Pseudocercosporella capsellae, in Western Australia
Article
Gunasinghe, N., You, M. P. and Barbetti, M. J.. 2016. "Phenotypic and phylogenetic studies associated with the crucifer white leaf spot pathogen, Pseudocercosporella capsellae, in Western Australia." Plant Pathology. 65 (2), pp. 205-217. https://doi.org/10.1111/ppa.12402
| Article Title | Phenotypic and phylogenetic studies associated with the crucifer white leaf spot pathogen, Pseudocercosporella capsellae, in Western Australia |
|---|---|
| ERA Journal ID | 2652 |
| Article Category | Article |
| Authors | Gunasinghe, N. (Author), You, M. P. (Author) and Barbetti, M. J. (Author) |
| Journal Title | Plant Pathology |
| Journal Citation | 65 (2), pp. 205-217 |
| Number of Pages | 13 |
| Year | 2016 |
| Publisher | John Wiley & Sons |
| Place of Publication | United Kingdom |
| ISSN | 0032-0862 |
| 1365-3059 | |
| Digital Object Identifier (DOI) | https://doi.org/10.1111/ppa.12402 |
| Web Address (URL) | http://onlinelibrary.wiley.com/doi/10.1111/ppa.12402/abstract |
| Abstract | Pseudocercosporella capsellae (white leaf spot disease) is an important disease on crucifers. Fifty four single-conidial isolates collected from Brassica juncea (Indian mustard), B. napus (oilseed rape), B. rapa (turnip), and Raphanus raphanistrum (wild radish) across Western Australia were investigated for differences in pathogenicity and virulence using cotyledon screening tests, genetic differences using Internal Transcribed Spacer (ITS) sequencing and phylogenetic analysis, and growth rates on potato dextrose, V8 juice and malt extract agars. All isolates from the four crucifer hosts were pathogenic on the three test species: B. juncea, B. napus and R. raphanistrum, but showed differences in levels of virulence. Overall, isolates from B. juncea, B. napus and B. rapa showed greatest virulence on B. juncea, least on R. raphanistrum and intermediate virulence on B. napus. Isolates from R. raphanistrum showed greatest virulence on B. juncea, least on B. napus and intermediate virulence on R. raphanistrum. Growth and production of a purple-pink pigment indicative of cercosporin was greatest on malt extract agar and cercosporin production on V8 juice agar was positively correlated with virulence of isolates on B. juncea and B. napus. ITS sequencing and phylogenetic analysis showed that isolates collected from B. napus, B. juncea and B. rapa, in general and with few exceptions, had a high degree of genetic similarity. In contrast, isolates from R. raphanistrum were clearly differentiated from isolate groups collected from Brassica hosts. P. capsellae reference isolates from other countries generally grouped into a single separate cluster, highlighting the genetic distinctiveness of Western Australian isolates. |
| Keywords | Pseudocercosporella capsellae, white leaf spot, genetic variation, phenotypic variation |
| ANZSRC Field of Research 2020 | 300409. Crop and pasture protection (incl. pests, diseases and weeds) |
| Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
| Byline Affiliations | University of Western Australia |
| Institution of Origin | University of Southern Queensland |
Permalink -
https://research.usq.edu.au/item/q3x95/phenotypic-and-phylogenetic-studies-associated-with-the-crucifer-white-leaf-spot-pathogen-pseudocercosporella-capsellae-in-western-australia
1350
total views12
total downloads1
views this month0
downloads this month
Export as
Related outputs
Diversity and pathogenicity of Fusarium spp. isolated from cultivated sorghum stems and roots in eastern Australia
Gunasinghe, Niroshini, Vaghefi, Niloofar, Shivas, Roger G., Tan, Yu Pei, Jordan, David, Mace, Emma and Martin, Anke. 2024. "Diversity and pathogenicity of Fusarium spp. isolated from cultivated sorghum stems and roots in eastern Australia." Plant Pathology. 73 (9), pp. 2563-2573. https://doi.org/10.1111/ppa.13985First report of Fusarium madaense as a cause of root and stalk rot on Sorghum bicolor in Australia
Gunasinghe, N., Vaghefi, N., Shivas, R. G., Tan, Y. P., Jordan, D., Mace, E., Cruickshank, A. and Martin, A.. 2023. "First report of Fusarium madaense as a cause of root and stalk rot on Sorghum bicolor in Australia." New Disease Reports. 47 (2). https://doi.org/10.1002/ndr2.12192IMA Genome - F16 Draft genome assemblies of Fusarium marasasianum, Huntiella abstrusa, two Immersiporthe knoxdaviesiana isolates, Macrophomina pseudophaseolina, Macrophomina phaseolina, Naganishia randhawae, and Pseudocercospora cruenta
Wingfield, Brenda D., De Vos, Lieschen, Wilson, Andi M., Duong, Tuan A., Vaghefi, Niloofar, Botes, Angela, Kharwar, Ravindra Nath, Chand, Ramesh, Poudel, Barsha, Aliyu, Habibu, Barbetti, Martin J., Chen, ShuaiFei, de Maayer, Pieter, Liu, FeiFei, Navathe, Sudhir, Sinha, Shagun, Steenkamp, Emma T., Suzuki, Hiroyuki, Tshisekedi, Kalonji A., ..., Wingfield, Michael J.. 2022. "IMA Genome - F16 Draft genome assemblies of Fusarium marasasianum, Huntiella abstrusa, two Immersiporthe knoxdaviesiana isolates, Macrophomina pseudophaseolina, Macrophomina phaseolina, Naganishia randhawae, and Pseudocercospora cruenta." IMA Fungus: the global mycological journal. 13 (1), pp. 1-22. https://doi.org/10.1186/s43008-022-00089-zFirst Report of Powdery Mildew Caused by Erysiphe cruciferarum on Brassica campestris var. pekinensis, B. carinata, Eruca sativa, E. vesicaria in Australia and on B. rapa and B. oleracea var. capitata in Western Australia
Gunasinghe, Niroshini, You, M. P., Lanoiselet, V., Eyres, N. and Barbetti, M. J.. 2013. "First Report of Powdery Mildew Caused by Erysiphe cruciferarum on Brassica campestris var. pekinensis, B. carinata, Eruca sativa, E. vesicaria in Australia and on B. rapa and B. oleracea var. capitata in Western Australia." Plant Disease: an international journal of applied plant pathology. 97 (9), pp. 1256-1256. https://doi.org/10.1094/PDIS-03-13-0299-PDNGenetic biofortification of wheat with zinc: Opportunities to fine-tune zinc uptake, transport and grain loading
Kamaral, Chandima, Neate, Stephen M., Gunasinghe, Niroshini, Milham, Paul J., Paterson, David J., Kopittke, Peter M. and Seneweera, Saman. 2021. "Genetic biofortification of wheat with zinc: Opportunities to fine-tune zinc uptake, transport and grain loading." Physiologia Plantarum. 174 (1), pp. 1-18. https://doi.org/10.1111/ppl.13612Dimorphism in Neopseudocercosporella capsellae, an Emerging Pathogen Causing White Leaf Spot Disease of Brassicas
Gunasinghe, Niroshini, Barbetti, Martin J., You, Ming Pei, Dehigaspitiya, Prabuddha and Neate, Stephen. 2021. "Dimorphism in Neopseudocercosporella capsellae, an Emerging Pathogen Causing White Leaf Spot Disease of Brassicas." Frontiers in Cellular and Infection Microbiology. 11, pp. 1-15. https://doi.org/10.3389/fcimb.2021.678231The use of Pantoea agglomerans and Flavobacterium sp. to control banana pathogens
Gunasinghe, R. Niroshini, Ikiriwatte, Chamari J. and Karunaratne, Anjani M.. 2004. "The use of Pantoea agglomerans and Flavobacterium sp. to control banana pathogens." Journal of Horticultural Science and Biotechnology. 79 (6), pp. 1002-1006. https://doi.org/10.1080/14620316.2004.11511852Interactions of Colletotrichum musae and Lasiodiplodia theobromae and their biocontrol by Pantoea agglomerans and Flavobacterium sp. in expression of crown rot of 'Embul' banana
Gunasinghe, W. K. R. Niroshini and Karunaratne, Anjani M.. 2009. "Interactions of Colletotrichum musae and Lasiodiplodia theobromae and their biocontrol by Pantoea agglomerans and Flavobacterium sp. in expression of crown rot of 'Embul' banana." BioControl: journal of the International Organisation for Biological Control. 54 (4), pp. 587-596. https://doi.org/10.1007/s10526-009-9210-4Hidden diversity of Macrophomina associated with broadacre and horticultural crops in Australia
Poudel, Barsha, Shivas, Roger G., Adorada, Dante L., Barbetti, Martin J., Bithell, Sean L., Kelly, Lisa A., Moore, Natalie, Sparks, Adam H., Tan, Yu Pei, Thomas, Geoff, van Leur, Joop and Vaghefi, Niloofar. 2021. "Hidden diversity of Macrophomina associated with broadacre and horticultural crops in Australia." European Journal of Plant Pathology. 161, pp. 1-23. https://doi.org/10.1007/s10658-021-02300-0Outstanding host resistance will resolve the threat from white leaf spot disease (Pseudocercosporella capsellae) to oilseed and vegetable Brassica spp. crops
Gunasinghe, Niroshini, You, Ming Pei, Banga, Surinder S., Banga, Shashi K. and Barbetti, Martin J.. 2017. "Outstanding host resistance will resolve the threat from white leaf spot disease (Pseudocercosporella capsellae) to oilseed and vegetable Brassica spp. crops." Australasian Plant Pathology. 46 (2), pp. 137-146. https://doi.org/10.1007/s13313-017-0470-7Unique infection structures produced by Pseudocercosporella capsellae on oilseed crops Brassica carinata, B. juncea and B. napus in Western Australia
Gunasinghe, N., You, M. P., Clode, P., Cawthray, G. R. and Barbetti, M. J.. 2017. "Unique infection structures produced by Pseudocercosporella capsellae on oilseed crops Brassica carinata, B. juncea and B. napus in Western Australia." Plant Pathology. 66 (2), pp. 304-315. https://doi.org/10.1111/ppa.12563Mechanisms of resistance in Brassica carinata, B. napus and B. juncea to Pseudocercosporella capsellae
Gunasinghe, N., You, M. P., Clode, P. L. and Barbetti, M. J.. 2016. "Mechanisms of resistance in Brassica carinata, B. napus and B. juncea to Pseudocercosporella capsellae." Plant Pathology. 65 (6), pp. 888-900. https://doi.org/10.1111/ppa.12484White Leaf Spot Caused by Neopseudocercosporella capsellae: A Re-emerging Disease of Brassicaceae
Gunasinghe, Niroshini, Barbetti, Martin J., You, Ming Pei, Burrell, Daniel and Neate, Stephen. 2020. "White Leaf Spot Caused by Neopseudocercosporella capsellae: A Re-emerging Disease of Brassicaceae." Frontiers in Cellular and Infection Microbiology. 10, pp. 1-16. https://doi.org/10.3389/fcimb.2020.588090Phylogeny and pathogenicity of Stemphylium species associated with Fabaceae in Australia
Vaghefi, Niloofar, Thompson, Susan M., Kimber, Rohan B.E., Thomas, Geoff J., Kant, Pragya, Barbetti, Martin J. and van Leur, Joop A.G.. 2020. "Phylogeny and pathogenicity of Stemphylium species associated with Fabaceae in Australia." Mycological Progress: international journal of fungal sciences. 19, pp. 381-396. https://doi.org/10.1007/s11557-020-01566-8First report of rice blast (Magnaporthe Oryzae) on rice (Oryza Sativa) in Western Australia
You, M. P., Lanoiselet, V., Wang, C. P., Shivas, R. G., Li, Y. P. and Barbetti, M. J.. 2012. "First report of rice blast (Magnaporthe Oryzae) on rice (Oryza Sativa) in Western Australia." Plant Disease: an international journal of applied plant pathology. 96 (8), pp. 1228-1229. https://doi.org/10.1094/PDIS-05-12-0420-PDNFirst report of Sarocladium Oryzae causing sheath rot on rice (Oryza sativa) in Western Australia
Lanoiselet, V., You, M. P., Li, Y. P., Wang, C. P., Shivas, R. G. and Barbetti, M. J.. 2012. "First report of Sarocladium Oryzae causing sheath rot on rice (Oryza sativa) in Western Australia." Plant Disease: an international journal of applied plant pathology. 96 (9), pp. 1382-1383. https://doi.org/10.1094/PDIS-04-12-0415-PDNNew host resistances to Pseudocercosporella capsellae and implications for white leaf spot management in Brassicaceae crops
Gunasinghe, Niroshini, You, Ming Pei, Li, Xi Xiang, Banga, Surinder S., Banga, Shashi K. and Barbetti, Martin J.. 2016. "New host resistances to Pseudocercosporella capsellae and implications for white leaf spot management in Brassicaceae crops." Crop Protection. 86, pp. 69-76. https://doi.org/10.1016/j.cropro.2016.04.014