Unique infection structures produced by Pseudocercosporella capsellae on oilseed crops Brassica carinata, B. juncea and B. napus in Western Australia

Article


Gunasinghe, N., You, M. P., Clode, P., Cawthray, G. R. and Barbetti, M. J.. 2017. "Unique infection structures produced by Pseudocercosporella capsellae on oilseed crops Brassica carinata, B. juncea and B. napus in Western Australia." Plant Pathology. 66 (2), pp. 304-315. https://doi.org/10.1111/ppa.12563
Article Title

Unique infection structures produced by Pseudocercosporella capsellae on oilseed crops Brassica carinata, B. juncea and B. napus in Western Australia

ERA Journal ID2652
Article CategoryArticle
AuthorsGunasinghe, N. (Author), You, M. P. (Author), Clode, P. (Author), Cawthray, G. R. (Author) and Barbetti, M. J.
Journal TitlePlant Pathology
Journal Citation66 (2), pp. 304-315
Number of Pages12
Year2017
PublisherJohn Wiley & Sons
Place of PublicationUnited Kingdom
ISSN0032-0862
1365-3059
Digital Object Identifier (DOI)https://doi.org/10.1111/ppa.12563
Web Address (URL)https://bsppjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/ppa.12563
Abstract

White leaf spot disease (Pseudocercosporella capsellae) is widespread across oilseed, vegetable and forage brassicas. Light (LM) and scanning electron (SEM) microscope studies were undertaken to investigate host–pathogen interactions on cotyledons of resistant and susceptible Brassica carinata, B. juncea and B. napus. Under LM, unique brown structures were present, particularly on susceptible genotypes, in two morphologically distinct forms: first, as thread-like structures within cortical tissue by 24 h post-inoculation (hpi) and secondly, as brown ropy strand structures either within cortical tissues (internal ropy strands), or extruded out through stomatal pores (ropy strand extrusions). Under LM, these brown structures were most prevalent in highly susceptible B. juncea ‘Vardan’ that had both a high incidence within cortical tissue (70%) and of ropy strand extrusions (73%), as did susceptible B. napus ‘Trilogy’ within cortical tissue (60%). Under SEM, both these genotypes showed thread-like structures smaller than hyphae forming highly branched networks and ropy strand-like structures. While there were fewer brown structures in susceptible B. carinata UWA #012 (35%), fine, thread-like structures forming networks were again prominent (SEM). In contrast, for resistant genotypes, brown structures (LM) were of very low frequency or absent; only 5% in resistant B. juncea ‘Dune’ and none in resistant B. napus ‘Hyola 42’ or highly resistant B. carinata ATC94129P. Under SEM, fine, threadlike structures were present in the resistant B. juncea ‘Dune’ and B. napus ‘Hyola 42’. Liquid chromatographic analyses of brown structures revealed that both internal ropy strands within cortical tissues and ropy strand extrusions contained the mycotoxin cercosporin.

Keywordscercosporin, extrusion structures, infection structures, resistance mechanisms, white leaf spot
ANZSRC Field of Research 2020310705. Mycology
310805. Plant pathology
300409. Crop and pasture protection (incl. pests, diseases and weeds)
Byline AffiliationsUniversity of Western Australia
Institution of OriginUniversity of Southern Queensland
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https://research.usq.edu.au/item/q6442/unique-infection-structures-produced-by-pseudocercosporella-capsellae-on-oilseed-crops-brassica-carinata-b-juncea-and-b-napus-in-western-australia

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