Mechanisms of resistance in Brassica carinata, B. napus and B. juncea to Pseudocercosporella capsellae

Article


Gunasinghe, N., You, M. P., Clode, P. L. and Barbetti, M. J.. 2016. "Mechanisms of resistance in Brassica carinata, B. napus and B. juncea to Pseudocercosporella capsellae." Plant Pathology. 65 (6), pp. 888-900. https://doi.org/10.1111/ppa.12484
Article Title

Mechanisms of resistance in Brassica carinata, B. napus and
B. juncea to Pseudocercosporella capsellae

ERA Journal ID2652
Article CategoryArticle
AuthorsGunasinghe, N. (Author), You, M. P. (Author), Clode, P. L. (Author) and Barbetti, M. J. (Author)
Journal TitlePlant Pathology
Journal Citation65 (6), pp. 888-900
Number of Pages13
Year2016
PublisherJohn Wiley & Sons
Place of PublicationUnited Kingdom
ISSN0032-0862
1365-3059
Digital Object Identifier (DOI)https://doi.org/10.1111/ppa.12484
Web Address (URL)https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/ppa.12484
Abstract

Studies were undertaken to compare susceptible and resistant host responses to Pseudocercosporella capsellae in cotyledons of Brassica carinata, B. juncea and B. napus in order to define the mechanisms of resistance in these three species. On both resistant and susceptible hosts, hyphal penetration was always through stomatal openings and without infection pegs or appressoria. On resistant B. carinata ATC94129P, up to 72% of spores disintegrated and, generally, germination (<22%) and germ tube lengths (<25 μm) were comparatively low. Resistant B. napus Hyola 42 had the lowest germination (8%) and susceptible B. carinata UWA#012 had the highest (51%). On resistant B. carinata ATC94129P, germ tube extension was impeded across 24–60 h post‐inoculation (hpi) and percentage stomatal penetration lower (4%) at 60 hpi compared with susceptible B. carinata UWA#012 (26%). Stomatal densities (stomata/14 757 μm2) on resistant B. juncea Dune (2·12) and B. napus Hyola 42 (1·62) were lower than for susceptible B. juncea Vardan (2·40) and B. napus Trilogy (2·03). Resistant B. carinata ATC94129P had greater stomatal density (1·89) than susceptible B. carinata UWA#012 (1·58). Overall, B. juncea had greater stomatal density (2·26) compared with B. napus (1·83) and B. carinata (1·74). In resistant B. carinata ATC94129P, P. capsellae induced 28% stomata to close, while in susceptible B. carinata UWA#012 no such closure was induced. Epicuticular wax crystalloids were present only on resistant B. carinata ATC94129P and probably also contribute towards resistance.

KeywordsBrassica carinata, Brassica juncea, Brassica napus, Pseudocercosporella capsellae, resistance mechanisms, white leaf spot
ANZSRC Field of Research 2020310705. Mycology
310805. Plant pathology
300409. Crop and pasture protection (incl. pests, diseases and weeds)
Byline AffiliationsUniversity of Western Australia
Institution of OriginUniversity of Southern Queensland
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