A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley

Article


Chen, Chunhong, Jost, Matthias, Outram, Megan A., Friendship, Dorian, Chen, Jian, Wang, Aihua, Periyannan, Sambasivam, Bartos, Jan, Holusova, Katerina, Dolezel, Jaroslav, Zhang, Peng, Bhatt, Dhara, Singh, Davinder, Lagudah, Evans, Park, Robert F. and Dracatos, Peter M.. 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley." Nature Communications. 14 (1). https://doi.org/10.1038/s41467-023-41021-2
Article Title

A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley

ERA Journal ID122966
Article CategoryArticle
AuthorsChen, Chunhong, Jost, Matthias, Outram, Megan A., Friendship, Dorian, Chen, Jian, Wang, Aihua, Periyannan, Sambasivam, Bartos, Jan, Holusova, Katerina, Dolezel, Jaroslav, Zhang, Peng, Bhatt, Dhara, Singh, Davinder, Lagudah, Evans, Park, Robert F. and Dracatos, Peter M.
Journal TitleNature Communications
Journal Citation14 (1)
Article Number5468
Number of Pages11
Year2023
PublisherNature Publishing Group
Place of PublicationUnited Kingdom
ISSN2041-1723
Digital Object Identifier (DOI)https://doi.org/10.1038/s41467-023-41021-2
Web Address (URL)https://www.nature.com/articles/s41467-023-41021-2
AbstractLeaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and haplotype divergence. In this study, we isolate the Rph7 gene using a fine mapping and RNA-Seq approach that is confirmed by mutational analysis and transgenic complementation. Rph7 is a pathogen-induced, non-canonical resistance gene encoding a protein that is distinct from other known plant disease resistance proteins in the Triticeae. Structural analysis using an AlphaFold2 protein model suggests that Rph7 encodes a putative NAC transcription factor with a zinc-finger BED domain with structural similarity to the N-terminal DNA-binding domain of the NAC transcription factor (ANAC019) from Arabidopsis. A global gene expression analysis suggests Rph7 mediates the activation and strength of the basal defence response. The isolation of Rph7 highlights the diversification of resistance mechanisms available for engineering disease control in crops.
Keywordsbarley; disease control; disease resistance; gene expression; mutation; protein
ANZSRC Field of Research 2020300103. Agricultural molecular engineering of nucleic acids and proteins
300105. Genetically modified field crops and pasture
300409. Crop and pasture protection (incl. pests, diseases and weeds)
Byline AffiliationsCommonwealth Scientific and Industrial Research Organisation (CSIRO), Australia
University of Sydney
Centre for Crop Health
Institute of Experimental Botany, Czech Republic
La Trobe University
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