A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley
Article
Chen, Chunhong, Jost, Matthias, Outram, Megan A., Friendship, Dorian, Chen, Jian, Wang, Aihua, Periyannan, Sambasivam, Bartos, Jan, Holusova, Katerina, Dolezel, Jaroslav, Zhang, Peng, Bhatt, Dhara, Singh, Davinder, Lagudah, Evans, Park, Robert F. and Dracatos, Peter M.. 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley." Nature Communications. 14 (1). https://doi.org/10.1038/s41467-023-41021-2
| Article Title | A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley |
|---|---|
| ERA Journal ID | 122966 |
| Article Category | Article |
| Authors | Chen, Chunhong, Jost, Matthias, Outram, Megan A., Friendship, Dorian, Chen, Jian, Wang, Aihua, Periyannan, Sambasivam, Bartos, Jan, Holusova, Katerina, Dolezel, Jaroslav, Zhang, Peng, Bhatt, Dhara, Singh, Davinder, Lagudah, Evans, Park, Robert F. and Dracatos, Peter M. |
| Journal Title | Nature Communications |
| Journal Citation | 14 (1) |
| Article Number | 5468 |
| Number of Pages | 11 |
| Year | 2023 |
| Publisher | Nature Publishing Group |
| Place of Publication | United Kingdom |
| ISSN | 2041-1723 |
| Digital Object Identifier (DOI) | https://doi.org/10.1038/s41467-023-41021-2 |
| Web Address (URL) | https://www.nature.com/articles/s41467-023-41021-2 |
| Abstract | Leaf rust, caused by Puccinia hordei, is one of the most widespread and damaging foliar diseases affecting barley. The barley leaf rust resistance locus Rph7 has been shown to have unusually high sequence and haplotype divergence. In this study, we isolate the Rph7 gene using a fine mapping and RNA-Seq approach that is confirmed by mutational analysis and transgenic complementation. Rph7 is a pathogen-induced, non-canonical resistance gene encoding a protein that is distinct from other known plant disease resistance proteins in the Triticeae. Structural analysis using an AlphaFold2 protein model suggests that Rph7 encodes a putative NAC transcription factor with a zinc-finger BED domain with structural similarity to the N-terminal DNA-binding domain of the NAC transcription factor (ANAC019) from Arabidopsis. A global gene expression analysis suggests Rph7 mediates the activation and strength of the basal defence response. The isolation of Rph7 highlights the diversification of resistance mechanisms available for engineering disease control in crops. |
| Keywords | barley; disease control; disease resistance; gene expression; mutation; protein |
| ANZSRC Field of Research 2020 | 300103. Agricultural molecular engineering of nucleic acids and proteins |
| 300105. Genetically modified field crops and pasture | |
| 300409. Crop and pasture protection (incl. pests, diseases and weeds) | |
| Byline Affiliations | Commonwealth Scientific and Industrial Research Organisation (CSIRO), Australia |
| University of Sydney | |
| Centre for Crop Health | |
| Institute of Experimental Botany, Czech Republic | |
| La Trobe University |
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