Characterization and Pathogenicity of Soilborne Pathogens in Gloriosa superba: Effects of Single- and Multiple-Pathogen Coinfection on Disease Responses

Article

Dhanabalan, Shanmuga Priya, Johnson, Iruthayasamy, Kumaresan, Parthiban V., Kandasamy, Rajamani, Natesan, Senthil, Periyannan, Sambasivam and Muthusamy, Karthikeyan. 2024. "Characterization and Pathogenicity of Soilborne Pathogens in Gloriosa superba: Effects of Single- and Multiple-Pathogen Coinfection on Disease Responses." Plant Disease: an international journal of applied plant pathology. 108 (11), pp. 3279-3287. https://doi.org/10.1094/PDIS-03-24-0496-RE
Article Title

Characterization and Pathogenicity of Soilborne Pathogens in Gloriosa superba: Effects of Single- and Multiple-Pathogen Coinfection on Disease Responses

ERA Journal ID2647
Article CategoryArticle
AuthorsDhanabalan, Shanmuga Priya, Johnson, Iruthayasamy, Kumaresan, Parthiban V., Kandasamy, Rajamani, Natesan, Senthil, Periyannan, Sambasivam and Muthusamy, Karthikeyan
Journal TitlePlant Disease: an international journal of applied plant pathology
Journal Citation108 (11), pp. 3279-3287
Number of Pages9
Year2024
PublisherAmerican Phytopathological Society
Place of PublicationUnited States
ISSN0191-2917
1943-7692
Digital Object Identifier (DOI)https://doi.org/10.1094/PDIS-03-24-0496-RE
Web Address (URL)https://apsjournals.apsnet.org/doi/epdf/10.1094/PDIS-03-24-0496-RE
Abstract

Glory lily (Gloriosa superba), an ornamental climbing plant, contains the bioactive compound colchicine, attracting attention from the pharmaceutical industry. However, soilborne pathogens have emerged as a serious threat to the cultivation of glory lily, leading to substantial economic losses in the southern parts of India. Among these, the three major pathogens are Macrophomina phaseolina, Fusarium oxysporum, and Agroathelia rolfsii, causing dry root rot (also referred to as charcoal rot), wilt, and stem rot, respectively. Here, we characterized these pathogens using morphological characteristics and phylogenetic analysis of DNA sequences related to the internal transcribed spacer of ribosomal DNA, calmodulin (CAL), and translation elongation factor 1-alpha (TEF-1α). Furthermore, in the pathogenicity tests, the inoculation of M. phaseolina alone resulted in lesions measuring 7.54 ± 0.01 mm on tubers and 90% seedling mortality. This severity was comparable to the simultaneous inoculation of all three pathogens, indicating the prominence of dry root rot among soilborne diseases. This study marks the first detailed investigation of soilborne pathogens combined infection in G. superba, contributing to the understanding of fungal disease complexity in medicinal plants.

Keywordssingle and coinfection; wilt; collar rot; disease complex; Gloriosa superba; root rot
Contains Sensitive ContentDoes not contain sensitive content
ANZSRC Field of Research 2020300103. Agricultural molecular engineering of nucleic acids and proteins
Public Notes

Files associated with this item cannot be displayed due to copyright restrictions.

Byline AffiliationsTamil Nadu Agricultural University, India
School of Agriculture and Environmental Science
Centre for Crop Health
Permalink -

https://research.usq.edu.au/item/zv081/characterization-and-pathogenicity-of-soilborne-pathogens-in-gloriosa-superba-effects-of-single-and-multiple-pathogen-coinfection-on-disease-responses

Log in to edit
  • 18
    total views
  • 0
    total downloads
  • 2
    views this month
  • 0
    downloads this month

Export as

Related outputs

Global transcriptome changes during growth of a novel Penicillium coffeae isolate on the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici
Wess, Jack, Hu, Yiheng, Periyannan, Sambasivam, Jones, Ashley and Rathjen, John P.. 2025. "Global transcriptome changes during growth of a novel Penicillium coffeae isolate on the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici." Fungal Genetics and Biology. 176. https://doi.org/10.1016/j.fgb.2024.103956
Loop-mediated isothermal amplification (LAMP) and suction spore trap-based assay for detection of mildew pathogens Podosphaera xanthii and Pseudoperonospora cubensis in cucurbits
Periyannan, Sambasivam, Palanisamy, Ananthi, Karthikeyan, Muthusamy, Jaffer, Shajith Bash, Dhanabalan, Shanmuga Priya, Shanmugavel, Bavish, Johnson, Iruthayasamy, Revathy, Natarajan, Boopathi, Narayanan Manikanda and Santosh, Ganapati Patil. 2025. "Loop-mediated isothermal amplification (LAMP) and suction spore trap-based assay for detection of mildew pathogens Podosphaera xanthii and Pseudoperonospora cubensis in cucurbits." Crop Protection. 187. https://doi.org/10.1016/j.cropro.2024.106964
Rapid and early detection of rice false smut pathogen Ustilaginoidea virens (Cook) (Takahashi) using Loop-Mediated Isothermal Amplification (LAMP)
Ayyanar, Kamalakannan, Sekhar, Yerramala Chandra, Chellappan, Gopalakrishnan, Iruthayasamy, Johnson, Subramanian, Rajesh, Lakshmi, Kandaswami Ramani Swarna, Thirumalai, Angayarkanni and Muthusamy, Karhikeyan. 2025. "Rapid and early detection of rice false smut pathogen Ustilaginoidea virens (Cook) (Takahashi) using Loop-Mediated Isothermal Amplification (LAMP)." Journal of Plant Pathology: rivista di patologia vegetale. 107, pp. 1091-1104. https://doi.org/10.1007/s42161-025-01881-7
Aflatoxin evaluation and integrated management strategies to minimize toxin contamination in maize and peanuts
Periyannan, Sambasivam, Karthikeyan, Muthusamy, Dhanabalan, Shanmuga Priya, Shanmugavel, Bavish, Jaffer, Shajith Basha, Marimuthu, Subbaiyan, Radhika, Krishnan, Nagappan, Elakkiya and Johnson, Iruthayasamy. 2025. "Aflatoxin evaluation and integrated management strategies to minimize toxin contamination in maize and peanuts." Journal of Agriculture and Food Research. 21. https://doi.org/10.1016/j.jafr.2025.101809
Biocontrol and growth promotion of groundnut by Pseudomonas putida GN1 against soilborne pathogens
Paramasivan, Mookkan, Ramjegathesh, Rajendran, Rajendran, Lingam, Thangam, Algarsamy, Indiragandhi, Pandian, Ravichandran, Venugopal, Johnson, Iruthayasamy, Karthikayan, Gandhi and Karthikeyan, Muthusamy. 2025. "Biocontrol and growth promotion of groundnut by Pseudomonas putida GN1 against soilborne pathogens." Archives of Phytopathology and Plant Protection. 58 (5), pp. 282-301. https://doi.org/10.1080/03235408.2025.2476217
Unravelling the Antibiotic Resistance: Molecular Insights and Combating Therapies
Palanikumar, Preethieswari, Nathan, Bharathi, Muthusamy, Karthikeyan, M, Suganthy, Natesan, Senthil and Sampathrajan, Vellaikumar. 2025. "Unravelling the Antibiotic Resistance: Molecular Insights and Combating Therapies." Applied Biochemistry and Biotechnology. https://doi.org/10.1007/s12010-025-05182-8
Comparative toxicity of certain medicinal plants against invasive chili thrips, Thrips parvispinus (Karny)
Elaiyabharathi, T, Satya bhanu, G, Saikumar, T, Suganthi, A, Kavitha, C and Karthikeyan, M. 2025. "Comparative toxicity of certain medicinal plants against invasive chili thrips, Thrips parvispinus (Karny)." Plant Science Today. 12 (1), pp. 1-8. https://doi.org/10.14719/pst.6328
Stacking beneficial haplotypes from the Vavilov wheat collection to accelerate breeding for multiple disease resistance
Tong, Jingyang, Tarekegn, Zerihun T., Jambuthenne, Dilani, Alahmad, Samir, Periyannan, Sambasivam, Hickey, Lee, Dinglasan, Eric and Hayes, Ben. 2024. "Stacking beneficial haplotypes from the Vavilov wheat collection to accelerate breeding for multiple disease resistance." Theoretical and Applied Genetics: international journal of plant breeding research. 137 (12). https://doi.org/10.1007/s00122-024-04784-w
Genome-wide atlas of rust resistance loci in wheat
Tong, Jingyang, Zhao, Cong, Liu, Dan, Jambuthenne, Dilani T., Sun, Mengjing, Dinglasan, Eric, Periyannan, Sambasivam K., Hickey, Lee T. and Hayes, Ben J.. 2024. "Genome-wide atlas of rust resistance loci in wheat." Theoretical and Applied Genetics: international journal of plant breeding research. 137 (8). https://doi.org/10.1007/s00122-024-04689-8
Harnessing rain hose technology for water-saving sustainable irrigation and enhancing blackgram productivity in garden land
Marimuthu, S., Kannan, S. Vallal, Pazhanivelan, S., Raju, M., Sivamurugan, A. P., Karthikeyan, M., Byrareddy, V. M., Mushtaq, S. and Surendran, U.. 2024. "Harnessing rain hose technology for water-saving sustainable irrigation and enhancing blackgram productivity in garden land." Scientific Reports. 14 (1). https://doi.org/10.1038/s41598-024-69655-2
Origin and evolution of the bread wheat D genome
Cavalet-Giorsa, Emile, González-Muñoz, Andrea, Athiyannan, Naveenkumar, Holden, Samuel, Salhi, Adil, Gardener, Catherine, Quiroz-Chávez, Jesús, Rustamova, Samira M., Elkot, Ahmed Fawzy, Patpour, Mehran, Rasheed, Awais, Mao, Long, Lagudah, Evans S., Periyannan, Sambasivam K., Sharon, Amir, Himmelbach, Axel, Reif, Jochen C., Knauft, Manuela, Mascher, Martin, ..., Krattinger, Simon G.. 2024. "Origin and evolution of the bread wheat D genome." Nature. 633, pp. 848-855. https://doi.org/10.1038/s41586-024-07808-z
Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance
Subramaniam, Subramaniam, Kadirvel, Palchamy and Periyannan, Sambasivam. 2024. "Wheat improvement through advances in single nucleotide polymorphism (SNP) detection and genotyping with a special emphasis on rust resistance." Theoretical and Applied Genetics: international journal of plant breeding research. 137 (10). https://doi.org/10.1007/s00122-024-04730-w
Unveiling Genetic Resistance to Crown Rot in Watkins Wheat Landrace Diversity Panel
Garg, Bharat, Rubayet, Md. Tanbir, Muthusamy, Karthikeyan, Subramaniam, Geethanjali, McDonald, Stephen, Balotf, Sadegh, Gardiner, Donald, Percy, Cassy and Periyannan, Sambasivam. 2024. "Unveiling Genetic Resistance to Crown Rot in Watkins Wheat Landrace Diversity Panel." 12th Australasian Soilborne Diseases Symposium (ASDS 2024). Kingscliff, Australia 26 - 30 Aug 2024 Australia.
Unleashing Bacillus species as versatile antagonists: Harnessing the biocontrol potentials of the plant growth-promoting rhizobacteria to combat Macrophomina phaseolina infection in Gloriosa superba
Dhanabalan, Shanmugapriya, Muthusamy, Karthikeyan, Iruthayasamy, Johnson, Kumaresan, Parthiban V., Ravikumar, Caroline, Kandasamy, Rajamani, Natesan, Senthil and Periyannan, Sambasivam. 2024. "Unleashing Bacillus species as versatile antagonists: Harnessing the biocontrol potentials of the plant growth-promoting rhizobacteria to combat Macrophomina phaseolina infection in Gloriosa superba." Microbiological Research. 283. https://doi.org/10.1016/j.micres.2024.127678
Comprehensive analysis of little leaf disease incidence and resistance in eggplant
Muthusamy, Muthusamy, Yogiraj, Gawande Priya, Elaiyabharath, Thiyagarajan, Jesu, Bonipas Antony John, Johnson, Iruthayasamy, Jaffer, Shajith Basha, Dhanabalan, Shanmuga Priya, Boopathi, Narayanan Manikanda, Marimuthu, Subbaiyan, Shobeiri Nejad, Hamid, Adorada, Dante L. and Periyannan, Sambasivam. 2024. "Comprehensive analysis of little leaf disease incidence and resistance in eggplant." BMC Plant Biology. 24, p. 576. https://doi.org/10.1186/s12870-024-05257-4
Sr65: a widely effective gene for stem rust resistance in wheat
Norman, Michael, Chen, Chunhong, Miah, Hanif, Patpour, Mehran, Sørensen, Chris, Hovmøller, Mogens, Forrest, Kerrie, Kumar, Subodh, Prasad, Pramod, Gangwar, Om Prakash, Bhardwaj, Subhash, Bariana, Harbans, Periyannan, Sambasivam and Bansal, Urmil. 2024. "Sr65: a widely effective gene for stem rust resistance in wheat." Theoretical and Applied Genetics: international journal of plant breeding research. 137 (1). https://doi.org/10.1007/s00122-023-04507-7
Nuclear exchange generates population diversity in the wheat leaf rust pathogen Puccinia triticina
Sperschneider, Jana, Hewitt, Tim, Lewis, David C., Periyannan, Sambasivam, Milgate, Andrew W., Hickey, Lee T., Mago, Rohit, Dodds, Peter N. and Figueroa, Melania. 2023. "Nuclear exchange generates population diversity in the wheat leaf rust pathogen Puccinia triticina." Nature Microbiology. 8 (11), pp. 2130 - 2141. https://doi.org/10.1038/s41564-023-01494-9
Wheat stripe rust resistance locus YR63 is a hot spot for evolution of defence genes – a pangenome discovery
Mackenzie, Amy, Norman, Michael, Gessese, Mesfn, Chen, Chunhong, Sørensen, Chris, Hovmøller, Mogens, Ma, Lina, Forrest, Kerrie, Hickey, Lee, Bariana, Harbans, Bansal, Urmil and Periyannan, Sambasivam. 2023. "Wheat stripe rust resistance locus YR63 is a hot spot for evolution of defence genes – a pangenome discovery." BMC Plant Biology. 23 (1). https://doi.org/10.1186/s12870-023-04576-2
Editorial: Plant genetic and genomic resources for sustained crop improvement
Rangan, Parimalan, Henry, Robert, Wambugu, Peterson and Periyannan, Sambasivam. 2023. "Editorial: Plant genetic and genomic resources for sustained crop improvement." Frontiers in Plant Science. 14. https://doi.org/10.3389/fpls.2023.1266698
The Keys to Controlling Wheat Rusts: Identification and Deployment of Genetic Resistance
Norman, Michael, Bariana, Harbans, Bansal, Urmil and Periyannan, Sambasivam. 2023. "The Keys to Controlling Wheat Rusts: Identification and Deployment of Genetic Resistance." Phytopathology: International Journal of the American Phytopathological Society. 113 (4), pp. 667-677. https://doi.org/10.1094/PHYTO-02-23-0041-IA
Editorial: Advances in crop resistance breeding using modern genomic tools
Huang, Lin, Li, Yinghui, Chen, Shisheng, Periyannan, Sambasivam and Fahima, Tzion. 2023. "Editorial: Advances in crop resistance breeding using modern genomic tools." Frontiers in Plant Science. 14. https://doi.org/10.3389/fpls.2023.1143689
A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley
Chen, Chunhong, Jost, Matthias, Outram, Megan A., Friendship, Dorian, Chen, Jian, Wang, Aihua, Periyannan, Sambasivam, Bartos, Jan, Holusova, Katerina, Dolezel, Jaroslav, Zhang, Peng, Bhatt, Dhara, Singh, Davinder, Lagudah, Evans, Park, Robert F. and Dracatos, Peter M.. 2023. "A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley." Nature Communications. 14 (1). https://doi.org/10.1038/s41467-023-41021-2