Effect of L-arginine on cardiac function and fibrosis in mdx mice
Poster
Paper/Presentation Title | Effect of L-arginine on cardiac function and fibrosis in mdx mice |
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Presentation Type | Poster |
Authors | Hoey, Andrew (Author) and van Erp, Christel (Author) |
Editors | Crane, Denis and Monteith, Greg |
Journal or Proceedings Title | COMBIO 2006 |
Journal Citation | 38, p. 106 |
Year | 2006 |
Place of Publication | Australia |
Web Address (URL) of Paper | http://www.asbmb.org.au/combio2006/ |
Conference/Event | Combined ASBMB, ASPS, AuPS, ANZSCDB, NZSBMB, NZSPP (2006) |
Event Details | Combined ASBMB, ASPS, AuPS, ANZSCDB, NZSBMB, NZSPP (2006) Event Date 24 to end of 28 Sep 2006 Event Location Brisbane, Australia |
Abstract | The primary cause of Duchenne Muscular Dystrophy, a condition that affects 1 in 3500 live male births, is a deficiency of the protein dystrophin. This deficiency leads to downregulation of the neuronal nitric oxide synthase (nNOS) and lowered production of nitric oxide. This study investigated the effects of L-arginine, the substrate of nNOS, on cardiac function and fibrosis in mdx mice, a widely used animal model for Duchenne Muscular Dystrophy. Six month old mice received 5 mg/g body weight L-arginine by daily oral gavage for six months. At the completion of treatment, mice were anaesthetised with sodium pentobarbitone (70 mg/kg, ip) prior to euthanasia by excision of the heart. Cardiac function was assessed with the Langendorff technique at a perfusion pressure of 80 mmHg. Mdx mice had an impaired left ventricular developed pressure relative to C57BL10ScSn control mice, but this difference was not evident in L-arginine treated mdx mice. The L-arginine treated mdx mice also showed an increased coronary flow and reduced diastolic stiffness relative to untreated mdx mice (P<0.05). This reduction in stiffness was associated with a significant reduction in cardiac collagen measured as the percent of left ventricular area stained with the collagen specific dye picrosirius red (P<0.05). These results indicate that chronic treatment with L-arginine produces the beneficial effects of improving function and reducing fibrosis in the dystrophin-deficient heart. |
Keywords | Duchenne Muscular Dystrophy, L-arginine, cardiac function, fibrosis, mdx mice |
ANZSRC Field of Research 2020 | 310999. Zoology not elsewhere classified |
Public Notes | Poster presentation. Abstract only available. No evidence of copyright restrictions on web site. |
Byline Affiliations | Department of Biological and Physical Sciences |
https://research.usq.edu.au/item/9xxzq/effect-of-l-arginine-on-cardiac-function-and-fibrosis-in-mdx-mice
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