A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction

Article


Trebbin, Andrea L. and Hoey, Andrew J.. 2009. "A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction." Muscle and Nerve. 39 (5), pp. 603-608. https://doi.org/10.1002/mus.21215
Article Title

A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction

ERA Journal ID14530
Article CategoryArticle
AuthorsTrebbin, Andrea L. (Author) and Hoey, Andrew J. (Author)
Journal TitleMuscle and Nerve
Journal Citation39 (5), pp. 603-608
Number of Pages6
Year2009
Place of PublicationUnited States
ISSN0148-639X
1097-4598
Digital Object Identifier (DOI)https://doi.org/10.1002/mus.21215
Abstract

The mdx mouse mutation arises from a C-to-T point mutation, which terminates the translation of dystrophin and results in the loss of a functional dystrophin protein. mdx mice are used widely in studies of the role of dystrophon and of potential treatments for Duchenne muscular dystrophy, thus accurate genotyping is essential. Current methods require labor-intensive efforts and can often lead to misconstrued results. This study describes a simple and highly reliable, sensitive, and user-friendly, high-resolution melt (HRM) assay that is able to utilize DNA obtained from a variety of sources in order to genotype the known sequence variant of the mdx mouse.

KeywordsDuchenne muscular dystrophy (DMD); genotyping; high-resolution melting (HRM); mdx mutation; single-nucleotide polymorphism (SNP)
ANZSRC Field of Research 2020319999. Other biological sciences not elsewhere classified
310503. Developmental genetics (incl. sex determination)
320213. Medical genetics (excl. cancer genetics)
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Byline AffiliationsCentre for Systems Biology
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