A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction
Article
Article Title | A novel and simple method for genotyping the mdx mouse using high-resolution melt polymerase chain reaction |
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ERA Journal ID | 14530 |
Article Category | Article |
Authors | Trebbin, Andrea L. (Author) and Hoey, Andrew J. (Author) |
Journal Title | Muscle and Nerve |
Journal Citation | 39 (5), pp. 603-608 |
Number of Pages | 6 |
Year | 2009 |
Place of Publication | United States |
ISSN | 0148-639X |
1097-4598 | |
Digital Object Identifier (DOI) | https://doi.org/10.1002/mus.21215 |
Abstract | The mdx mouse mutation arises from a C-to-T point mutation, which terminates the translation of dystrophin and results in the loss of a functional dystrophin protein. mdx mice are used widely in studies of the role of dystrophon and of potential treatments for Duchenne muscular dystrophy, thus accurate genotyping is essential. Current methods require labor-intensive efforts and can often lead to misconstrued results. This study describes a simple and highly reliable, sensitive, and user-friendly, high-resolution melt (HRM) assay that is able to utilize DNA obtained from a variety of sources in order to genotype the known sequence variant of the mdx mouse. |
Keywords | Duchenne muscular dystrophy (DMD); genotyping; high-resolution melting (HRM); mdx mutation; single-nucleotide polymorphism (SNP) |
ANZSRC Field of Research 2020 | 319999. Other biological sciences not elsewhere classified |
310503. Developmental genetics (incl. sex determination) | |
320213. Medical genetics (excl. cancer genetics) | |
Public Notes | Files associated with this item cannot be displayed due to copyright restrictions. |
Byline Affiliations | Centre for Systems Biology |
https://research.usq.edu.au/item/9z62v/a-novel-and-simple-method-for-genotyping-the-mdx-mouse-using-high-resolution-melt-polymerase-chain-reaction
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