An investigation on the antimicrobial activity of Andrographis paniculata extracts and Andrographolide in vitro

Article

Xu, Youhong, Marshall, Raymond L. and Mukkur, T. K. S.. 2006. "An investigation on the antimicrobial activity of Andrographis paniculata extracts and Andrographolide in vitro." Asian Journal of Plant Sciences. 5 (3), pp. 527-530.
Article Title

An investigation on the antimicrobial activity of Andrographis paniculata extracts and Andrographolide in vitro

ERA Journal ID2694
Article CategoryArticle
AuthorsXu, Youhong (Author), Marshall, Raymond L. (Author) and Mukkur, T. K. S. (Author)
Journal TitleAsian Journal of Plant Sciences
Journal Citation5 (3), pp. 527-530
Number of Pages4
Year2006
Place of PublicationPakistan
ISSN1682-3974
1812-5697
Abstract

An aqueous and two ethanolic extracts of Andrographis paniculata, used in traditional Chinese, Thai and Indian medicine and andrographolide, an active principle of Andrographis paniculata, were investigated for their antimicrobial activity against nine bacterial species including Salmonella Typhumurium, Escherichia coli, Shigella sonnei, Staphylocuccus aureus, Pseudomonas Aeruginosa, Streptococcus pneumoniae, Streptococcus pyogenes, Legionella pneumophila and Bordetella pertussis, using the disc diffusion method. Of all tested concentrations, direct antimicrobial activity of the two ethanolic Andrographis paniculata extracts was observed for only two human pathogens, Legionella pneumophila and Bordetella pertussis. However, no antibacterial activity of andrographolide against any of the pathogens tested in this investigation was observed. Given that the TLC of Andrographis paniculata extracts showed that andrographolide was present in all the three Andrographis paniculata extracts, it was concluded that the observed antimicrobial activity was due to other active principle(s) present in the extracts used in this investigation.

KeywordsAndrographis paniculata; andrographolide; antimicrobial; disc diffusion, medicinal plant extracts
ANZSRC Field of Research 2020310701. Bacteriology
420803. Traditional Chinese medicine and treatments
310702. Infectious agents
Public Notes

Files associated with this item cannot be displayed due to copyright restrictions.

Byline AffiliationsFaculty of Sciences
Department of Biological and Physical Sciences
Permalink -

https://research.usq.edu.au/item/9zwwz/an-investigation-on-the-antimicrobial-activity-of-andrographis-paniculata-extracts-and-andrographolide-in-vitro

Log in to edit
  • 2721
    total views
  • 9
    total downloads
  • 7
    views this month
  • 0
    downloads this month

Export as

Related outputs

Construction and preliminary immunobiological characterization of a novel, non-reverting, intranasal live attenuated whooping cough vaccine candidate
Cornford-Nairns, R., Daggard, G. and Mukkur, T.. 2012. "Construction and preliminary immunobiological characterization of a novel, non-reverting, intranasal live attenuated whooping cough vaccine candidate." Journal of Microbiology and Biotechnology. 22 (6), pp. 856-865. https://doi.org/10.4014/jmb.1108.08003
Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis
Farrell, David John, Daggard, Grant Edward and Mukkur, Trilochan Kanwaljit Singh. 2001. Shared primer PCR combined with hybridisation for the detection of Bordetella Pertussis and B. Parapertussis. WO/2001/90405
Defective entities and uses therefor
Cornford-Nairn, Renee, Daggard, Grant Edward, Mukkur, Trilochan Kanwaljit Singh and Rossetti, Tony Robert. 2008. Defective entities and uses therefor. AU2002357406
Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection
Fry, Scott R., Chen, Austen Y., Daggard, Grant and Mukkur, Trilochan K. S.. 2008. "Parenteral immunization of mice with a genetically inactivated pertussis toxin DNA vaccine induces cell-mediated immunity and protection." Journal of Medical Microbiology. 57 (1), pp. 28-35. https://doi.org/10.1099/jmm.0.47527-0
Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice
Chen, Austen Y., Fry, Scott R., Daggard, Grant E. and Mukkur, Trilochan K. S.. 2008. "Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice." Vaccine. 26 (34), pp. 4372-4378. https://doi.org/10.1016/j.vaccine.2008.06.005
Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice
Chen, Austen Y., Fry, Scott Robert, Forbes-Faulkner, Judy, Daggard, Grant and Mukkur, T. K. S.. 2006. "Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice." Veterinary Microbiology. 114 (3-4), pp. 252-259. https://doi.org/10.1016/j.vetmic.2005.12.009
Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice
Chen, Austen Y., Fry, Scott R., Forbes-Faulkner, Judy, Daggard, Grant and Mukkur, T. K. S.. 2006. "Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice." Journal of Medical Microbiology. 55 (7), pp. 923-929. https://doi.org/10.1099/jmm.0.46088-0
(Z,Z)-3,6-Bis[(3,4,5-trimethoxyphenyl)-methylene]piperazine-2,5-dione
Loughlin, Wendy A., Marshall, Raymond L., Fairfull-Smith, Kathryn E. and Healy, Peter C.. 2005. "(Z,Z)-3,6-Bis[(3,4,5-trimethoxyphenyl)-methylene]piperazine-2,5-dione." Acta Crystallographica Section E: Crystallographic Communications. 61 (5), pp. 1443-1445. https://doi.org/10.1107/S1600536805012316
Adaptive immune response-modifying and antimicrobial properties of Andrographis paniculata and andrographolide
Xu, Youhong. 2009. Adaptive immune response-modifying and antimicrobial properties of Andrographis paniculata and andrographolide. PhD Thesis Doctor of Philosophy. University of Southern Queensland.
Modulation of immune response in mice immunised with an inactivated Salmonella vaccine and gavaged with Andrographis paniculata extract or andrographolide
Xu, Youhong, Chen, Austen Y., Fry, Scott R., Barrow, Russell A., Marshall, Raymond L. and Mukkur, T. K. S.. 2007. "Modulation of immune response in mice immunised with an inactivated Salmonella vaccine and gavaged with Andrographis paniculata extract or andrographolide." International Immunopharmacology. 7 (4), pp. 515-523. https://doi.org/10.1016/j.intimp.2006.12.008
Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis
Farrell, D. J., McKeon, M., Daggard, G., Loeffelholz, M. J., Thompson, C. J. and Mukkur, T. K. S.. 2000. "Rapid-cycle PCR method to detect Bordetella pertussis that fulfills all consensus recommendations for use of PCR in diagnosis of pertussis." Journal of Clinical Microbiology. 38 (12), pp. 4499-4502.
Nested duplex PCR to detect bordetella pertussis and bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan Southeast Queensland, Australia
Farrell, D. J., Daggard, G. and Mukkur, T. K. S.. 1999. "Nested duplex PCR to detect bordetella pertussis and bordetella parapertussis and its application in diagnosis of pertussis in nonmetropolitan Southeast Queensland, Australia." Journal of Clinical Microbiology. 37 (3), pp. 606-610.
Computer assisted organic synthesis/retrosynthetic analysis
Tither, Darren and Marshall, Raymond L.. 2005. "Computer assisted organic synthesis/retrosynthetic analysis." Arnold, Dennis and Atefi, Farzad (ed.) Brisbane Biological and Organic Chemistry Symposium (BBOCS 2005). Brisbane, Australia 25 Nov 2005 Brisbane, Queensland.
A metabolite-deficient mutant of Bordetella pertussis that induces secretory IgA and cell-mediated immunity in mice vaccinated by the intranasal route
Mukkur, T. K. S., Cornford-Nairn, Renee A., Rossetti, T., Kershaw, S., Fry, Scott Robert, Xu, Youhong and Daggard, Grant A.. 2005. "A metabolite-deficient mutant of Bordetella pertussis that induces secretory IgA and cell-mediated immunity in mice vaccinated by the intranasal route." Fang, Ferric C. (ed.) 105th American Society for Microbiology: General Meeting. Atlanta, Georgia 05 - 09 Jun 2005 Washington.